Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

... Consequently, the molecular studies of the interaction of SAgs with their s pecifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... bearing T-cell subsets are expanded by interaction with SSA. Some authors indicated clonal expansion of T-cells bearing human Vb3, Vb12, Vb17 and...

Ngày tải lên: 07/03/2014, 16:20

... glucose standard curve and the nmoles of XGOs produced was determined for both the PAHBAH and the bicinchoninic acid reducing sugar methods. For the PAHBAH method, the nmol of XGOs ¼ nmol of glucose ... further washing with 0.04% NaN 3 . Pieces of the gels were rinsed with water, blotted on filter paper and chopped finely with a razor blade. The dry mass of...

Ngày tải lên: 23/03/2014, 21:20

... 7.2, and the stability of the enzyme was examined by keeping the pure preparation for 5, 15 and 30 min at 40, 50, 60 and 65 °C before the assay. The effect of inhibitors was established with the ... to strengthen the electron-withdrawing capacity of the co-enzyme [15]. The active site Lys258 transfers a proton from the amino acid substrate to the cofactor...

Ngày tải lên: 14/03/2014, 23:20

... by the expression of mutant huntingtin and the exact mechanism by which mutant huntingtin alters the function of such factors. This work describing the gene structure of CB1 and i ts pattern of ... full complement of mouse huntingtin. Transgenic R 6 mouse models differ in the length of the CAG repeat within exon 1 of the human HD transgene and site of t...

Ngày tải lên: 16/03/2014, 18:20

... 6); and #PFLF-1 and #PFLR-1 (lanes 7 and 8). (B) Schematic organization of the CrPrx gene. The asterisk indicates the position of the codon encoding the first amino acid of the mature protein, and ... during the first hour. In the case of UV and cold exposure, the maximum transcript level was observed at 12 and 24 h, respectively. On the other hand, a gra...

Ngày tải lên: 23/03/2014, 09:21

... reading and help in preparation of this paper. REFERENCES 1. Venulet, J. & Van-Etten, R.L. (1970) Biochemistry and pharma- cology of the nitro and nitroso group. In The Chemistry of the Nitro and ... pre-equilibrated with 10 m M sodium phosphate buffer (pH 8.0). The column was first washed with the same buffer containing 0.2 M NaCl, and then the proteins wer...

Ngày tải lên: 31/03/2014, 08:20

... products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation without ... isolated and its genomic structure and expression analysis investi- gated. This work will pave the way for further investigation of the biological function of...

Ngày tải lên: 20/02/2014, 02:21

... in catalysis in the bacterial enzyme and the corresponding amino acids in the human protein are pointed with arrows. The mean hydrophobicity values of residues within the window centered on the pointed ... superimposing the ACMSD model with the bacterial ACMSD–Zn 2+ complex and past- ing the zinc ion in the predicted human structure. Figures were generate...

Ngày tải lên: 19/02/2014, 02:20

... of Science, The Blix Family Foundation and grants from the University of Oslo and the National Cancer Institute of the USA (R01-CA81150). The experiments were performed in accordance with all regu- lations ... increased the phosphorylation of the endogenous 140 kDa and  60 kDa isoforms of Ras-GRF1 (Fig. 2A, left panel) and of recombinant HA-Ras-GRF1 (Fig....

Ngày tải lên: 19/02/2014, 18:20

... flavonoid biosynthesis. We report the expression of highly active Citrus unshiu FLS in Escherichia coli and the purification of the labile enzyme by a modified protocol devised for the isolation of Petunia ... for FHT [9], and the protein concentration was adjusted to 0.371 mgÆmL )1 in the sample. The documented spectra show the accumulation of 10 scans with 50 nm...

Ngày tải lên: 21/02/2014, 03:20