Báo cáo khoa học: Neuroserpin Portland (Ser52Arg) is trapped as an inactive intermediate that rapidly forms polymers Implications for the epilepsy seen in the dementia FENIB ppt

... Neuroserpin Portland (Ser52Arg) is trapped as an inactive intermediate that rapidly forms polymers Implications for the epilepsy seen in the dementia FENIB Didier Belorgey 1 , ... neuroserpin was comparable to that obtained for other serpins, including a 1 -antitrypsin and a 1 -antichymo- trypsin [19,20]. In comparison, the spectrum of S...

Ngày tải lên: 07/03/2014, 16:20

... there is a blue shift in the 380 nm peak and subsequent small increase in absorbance at 340 nm, although the increase is much less than that expected for the amount of NADPH added (the increase ... nm and 400 and 500 nm. There is also an increase and blue shift in the peak which corresponds to the 380 and 360 nm peaks of the E and EH 2 forms, respect- ively. Th...

Ngày tải lên: 07/03/2014, 17:20

... 68% of the decrease in J o , 68% of the decrease in J p , 56% of the increase in the QH 2 ⁄ Q ratio, 70% of the increase in Dw, 72% of the increase in the ATP in ⁄ ADP in ratio, and 59% of the decrease ... were determined from yeast hexo- kinase kinetics as described [16]. As hexokinase kinetics were determined in medium containing creatine and creat- ine p...

Ngày tải lên: 19/02/2014, 05:20

... microsequencing and cloning of spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzyme Christian Winkler, Britta Delvos, William Martin and Katrin Henze Institute ... reaction was initi- ated by addition of 0.4 mmd-fructose 6-phosphate, and activity was determined by decrease in absorbance at 340 nm. For discrimination between the chloroplast and cy...

Ngày tải lên: 07/03/2014, 11:20

... domain binding motif in their C-termini. PDZ domains have been established as sites for protein–protein inter- action and play a central role in organizing diverse cell signaling assem- blies. In ... grouped into three main classes of PDZ binding motifs, occurring at the C-terminus of the interacting proteins [16]. We scanned the human olfactory receptor repertoire for putat...

Ngày tải lên: 18/02/2014, 13:20

... specific binding, the antisera were preincubated with purified recombinant Cyp4x1 protein, resulting in the ablation of detection of the 55-kDa protein in brain (Fig. 5C). This shows that the antisera ... the corresponding protein is translated in vivo. An antibody specific for the mouse Cyp4x1 protein was developed, as an antibody raised against human CYP4x1 showed...

Ngày tải lên: 19/02/2014, 07:20

... thus excluding any interchange of subunits. Nevertheless, it was apparent that the fluorescence intensity of the ac bands in all reoxidized samples was rather weak, a lthough the SDS band was very intense. ... cA285C was generated by standard PCR with pKH7 as template DNA and using KpnIandSacI for transferring the PCR product into pKH7 (resulting in plasmid pMM9). The mutat...

Ngày tải lên: 19/02/2014, 16:20

... involved in the synthesis of fatty acids in plastids, including the stearoyl acyl carrier protein desaturase SSI2, and the plastidic long-chain acyl-CoA synthetase LACS9 (for a complete list of co-expressed ... forma- tion by introducing the oxygen between carbon 9 and 10 of oleic acid before incorporation of the monomer into the cutin. In this context, it is interesting...

Ngày tải lên: 07/03/2014, 03:20

... coordinately regulated. Experimental procedures Cell culture, transfection and luciferase assay The RAW-264.7 cell line was obtained from ATCC (Manassas, VA) and maintained as described. Media was DMEM ... underlined) in mouse is nonhomologous to human (human U-358, GenBank #AF024710). Following muta- genesis, the sequence of the VEGF-dL-luc was verified by sequencing on both stran...

Ngày tải lên: 07/03/2014, 12:20

... molecules as well as other enzymes, such as inducible nitric oxide synthase and cyclooxengenase-2, NF-jB may provide an attractive target for therapeutic intervention in inflammatory diseases [2]. ... agents that trigger exacerbation in airway diseases [15]. This epithelium is biosynthetically active and acts as a source of multiple in ammatory cytokines, chemokines, prost...

Ngày tải lên: 07/03/2014, 15:20