Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

... epitope to the C terminus of the CRFR1 isoforms (Fig. 1C). The predicted masses of the isoforms without/with V5 tag are as follows: CRFR1a (47.7/52 kDa), CRFR1e1 (10.8/ 15.1 kDa), CRFR1e2 (28.1/32.4 ... excitability and associative learning. J Neurosci. 23, 700–707. 2830 A. Pisarchik and A. Slominski (Eur. J. Biochem. 271) Ó FEBS 2004 Molecular and functional char...

Ngày tải lên: 07/03/2014, 15:20

... induction of the culture with IPTG, which matched the molecular mass predicted from the amino acid sequence of AK2 protein. The purification and functional folding of L. donovani AK from the E. coli cell ... catalyses the reversible transfer of the c-phosphate group from ATP to AMP, releasing two molecules of ADP. This study describes cloning and function...

Ngày tải lên: 21/02/2014, 00:20

... we focus on the biochemical and functional characterization of the novel VBARP-L and VBARP-S transcripts. Bioinformatics analyses show that VBARP-L and VBARP-S are comprised of 11 and 9 exons, ... distribution of VBARP is independent of the cycling stage of the cells. Identification of biological function(s) of VBARP using a siRNA assay To examine the role...

Ngày tải lên: 07/03/2014, 21:20

... mostly by serines or glycines. The coordination of Ca 2+ by the )Z glutamate is the prime reason for the move- ment of the outgoing helix and the conformational change of EF-hand proteins upon Ca 2+ binding ... concluded that EF-hand 1 of the Iba proteins is not capable of functional Ca 2+ binding. Function of EF-hand 2 In both Iba1 crystal structures, the loop...

Ngày tải lên: 16/03/2014, 06:20

... comprised of three cells, the excretory cell, the duct cell and the pore cell [8]. Destruction of any of these cells by laser abla- tion causes the animal to swell with fluid and die [9]. The excretory ... anteriorly and posteriorly from the cell body. A fluid-filled excretory canal is sur- rounded by the cell cytoplasm. The basal pole of the cell faces either...

Ngày tải lên: 30/03/2014, 03:20

... especially in the mammalian forebrain, throughout the lifespan of the animal. This amino acid binds to the Gly-binding site of the N-methyl-d-aspartate subtype of the Glu recep- tor in the brain, and potentiates ... a Grant-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan, a Grant-in-Aid for Scientific Research from the Japan Socie...

Ngày tải lên: 30/03/2014, 04:20

... iodide was from Molecular Probes (Leiden, the Netherlands). Antibodies recognizing the C-terminus of P2Y 1 and P2Y 12 receptors, Tween 20 and cell-dissociation solution were obtained from Alomone Laboratories ... results again high- light the stimulatory role of P2Y 12 in mitogen-activated protein kinase and Akt kinase signaling pathways. On the other hand, the func...

Ngày tải lên: 30/03/2014, 08:20

... 5). Because of the relative insensitivity of front-end desaturases and elongases to the presence of an x3-double bond and of x3-desaturases to the structure of the carboxyl end of the fatty acids, the ... the synthesis of EPA in P. tricornutum and that the preferred route relied on intermediates of both the x6- and the x3-pathway (see Fig. 1). The...

Ngày tải lên: 31/03/2014, 09:20

... sequence of HP1287 shows 33% identity and 51% similarity to the TenA protein from B. subtilis. The 3D structure of the monomer is quite similar to that of the other members of the TenA family of known ... The superposition of the Ca atoms of one monomer with that of the other members of the family gives a rmsd of 1.7, 1.3 and 1.4 A ˚ for models 1RTW...

Ngày tải lên: 16/03/2014, 00:20

... limited set of enzymes involved in amino acid synthesis of Ser from Gly and Ala from Cys and conversions between Asp and Asn and between Glu and Gln [49]. Serine metabolic pathways are often absent ... mutagenesis of Trp139 to Gly resulted in the dissociation of the tetramer to a pair of dimers and in the loss of cooperativity in serine binding and inhibiti...

Ngày tải lên: 19/02/2014, 13:20