Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

... Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding Cyril Bar ˇ inka 1 , Petra ... residues and other amino acids situated in the vicinity of these residues. The results confirmed the importance of the amino acid residues, all located at the putative cat...

Ngày tải lên: 07/03/2014, 15:20

... the strain 168 [22] as the template and with the following primers: 5¢-pyrH,5¢-GGGGCATATGGAA AAACCAAAATACAAACGTATCGTATTA-3¢ ;and 3¢-pyrH,5¢-CCCCCTCGAGTTATTTCCCCCTCACGA TCGTTCCGATTGATTCAC-3¢. The ... as the ATP-binding site, while the N-terminal domain would contain the cosubstrate and the effector binding sites. The dimerization interface is composed of a-helices and...

Ngày tải lên: 31/03/2014, 07:20

... all the evidence suggests that sex determination might disobey the conventional rules of evolutionary conservation. The common picture emerging here is that the genes at the top of the cascade are ... or the germ cells indicated that mouse Dmrt1 is indeed required for radial migration of germ cells and survival of gonocytes. It is also required autonomously...

Ngày tải lên: 14/02/2014, 19:20

... epigenetic modifications. The two chromatin states, of gene-active unwrapped euchromatin at the nucleoplasm, and of gene-silenced condensed heterochromatin at the vicinity of the INM are circled. In the latter ... degeneration of the caudate nucleus, putamen, and occasionally the globus pallidus, with little involve- ment of the rest of the brain. The...

Ngày tải lên: 19/02/2014, 02:20

... function of wild-type eEF2 thereby reducing the rate of protein synthesis and the growth rate of the transformed cells. As shown in Fig. 3B, the doubling rate was only mar- ginally affected by the ... (C). The cells were allowed to grow at 30 °C under vigorous shaking. The attenuance of the yeast cultures was measured at 600 nm at the intervals indicated (A–C...

Ngày tải lên: 07/03/2014, 05:20

... region of the genome. The D loop is created by the displacement of one of the parental strands by 0.5–1 kb nascent DNA strand needed for the replication of the L strand. The 0.8–1 kb long D loop ... transcription termin- ation at the end of the 16 S rRNA gene. The putative tridecamer terminator sequence has been mapped to the 5¢ end of the tRNA Leu gene...

Ngày tải lên: 08/03/2014, 08:20

... appar- atus, as compared to the relatively straightforward prepar- ation of nuclei, is that the procedure comprises a series of density centrifugation steps as the physical properties of the material ... Despite the exceptional analytical power of this approach, system- atic limitations of the approach at the present state of the technology became apparent. There...

Ngày tải lên: 23/03/2014, 20:22

... groups. Attractive scenarios developed over the last couple of years are (a) the localized relaxation of chromatin at the site of DNA damage, mediated either by direct modifi- cation of histones ... to be relevant for the inhibitory effect of PARP-10 con- cerning c-Myc- and E1A-mediated cotransformation of rat embryo fibroblasts. Conclusion and outlook The field...

Ngày tải lên: 20/02/2014, 01:20

... 4A). The rapid forma- tion of the charge-transfer intermediate prevented accurate analysis of the rate constant for its formation by the stopped-flow method; the rate constant for col- lapse of the ... inhibitor rather than a substrate for this enzyme. The presence of Cb atom at posi- tion 26 therefore causes the substrate specificity of Table 2. Kinetic parame...

Ngày tải lên: 20/02/2014, 02:21

... showing that deletion of the N-domain of ClpA dramatically reduced the rate of degradation of GFP–ssrA and to a lesser extent casein [8,11], we speculated that the N-domain facilitates an early ... that the defect in RR ⁄ AA-mediated unfolding is not dependent on the global thermodynamic stability of the substrate, but rather correlates with local unfolding of the...

Ngày tải lên: 07/03/2014, 05:20