Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc

... in modulating the metal release from metallothionein in the absence of nitric oxide by Vallee, Maret and coworkers [22,37,38]. These authors reported increased metal release in the presence of ... tighter binding of cadmium than zinc in metallothioneins and the preference of zinc in the more flexible b-domain. In addition to the already described...

Ngày tải lên: 07/03/2014, 15:20

... cycles in such a combination do not touch each other). The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence ... vicinity of the steady state. We do this to investigate the stability of this state. In this way we obtain the in uence a small change in the conce...

Ngày tải lên: 19/02/2014, 16:20

... meth- ylamine are bound to the enzyme in this order, and N-methyl-l-alanine and then NADP + are released from the enzyme. Therefore, the kinetic mechanism of the enzyme is the same as those of conventional ... assay of fractions from the Blue-Sepharose column revealed that a 36-kDa protein was the enzyme exhibiting the NMAADH activity. The N-terminal amino-acid s...

Ngày tải lên: 19/02/2014, 16:20

... quantified by phosphorimaging of the band corresponding to the full- length protein, and normalizing to the Coomassie blue staining of the same band.) It was next demonstrated that the kinase eluted in fraction ... aldosterone and insulin, as well as intracellular signalling components such as protein kinase C and protein kinase A, were found to increase the in vivo phosp...

Ngày tải lên: 21/02/2014, 03:20

... Mutations in the 23S ribosomal RNA in the vicinity of the double hook binding or in the b-hairpin of protein L22 were shown to bypass the elongation arrest [72]. The swing of protein L22 hairpin tip ... puromycin derivatives in the active site as well as the 3¢ termini of the docked tRNA [1,3,5,6]. The motions of the tRNA molecules originating from th...

Ngày tải lên: 23/03/2014, 17:22

... present investigation examines the coupling of drug binding to ATP hydrolysis. Initial drug binding to the protein requires a high-affinity interaction with the drug binding site, followed by transition ... 10 ½ðlogIC 50 ÀLÞ n  ð1Þ where B is the maximal [ 3 H]daunomycin binding, B max is the maximal binding, B min is the minimum binding, IC 50 is the concentration of drug...

Ngày tải lên: 18/02/2014, 18:20

... location of eight cysteine residues and several other amino acids of the chitin-binding site (Fig. 2). The striking similarity between WAMPs and the chitin-binding domains of class I chitinases ... conserved. To the best of our knowledge, this is the first communication on such a replacement in the chitin-binding site. Analysis of chi- tin-binding properties of WAMPs...

Ngày tải lên: 07/03/2014, 02:20

... Nota- bly, in the presence of the neurotoxin, there was a delay in the lag time for fibrillation. The lag time increased from 74.9 h in case of a -synuclein alone to 86.8 and 93.6 h in the presence of ... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric pro...

Ngày tải lên: 14/02/2014, 19:20

... significant production of P i in the vicinity of the ATPase within the matrix. CYPD ablation or its inhibition by cyclosporin A increases the rate of respiration stimulated by arsenate in intact mitochondria Regarding ... and unbinding by cyclosporin A was demonstrated in the present study in intact mitochondria. This is the result of an imposing role of th...

Ngày tải lên: 14/02/2014, 19:20

... morphology of the strains used in this study. (A) Morphology of deletion strains. The indicated strains were grown in YPD to stationary phase. The cells were then fixed with formaldehyde and examined by ... vitro activity of acyl-CoA:ergosterol acyltransferase was determined in an are1 deletion background. The enzyme activity in the ste20D strain and the cla4D str...

Ngày tải lên: 18/02/2014, 13:20