Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricin B-chain Leu152; and Met234 of the ML1p and ML2p B-chains corresponding to the ricin B-chain Leu233). Estimation ... RIPs (such as ricin and abrin) [2], the mlp genes do not contain introns and they encode the toxic lectins in the form of a single chain pr...

Ngày tải lên: 07/03/2014, 15:20

... calcium binding domains (EF1–4) and calcineurin A binding domain are shown in the box. The dot in the EF1 box repre- sents the modified amino acids alanine (A) as compared to the oxygen containing-calcium ... unshaded. The amino acids marked by red, blue, green and pink lines indicates the putative casein kinase II, protein kinase C, the cAMP- and cGMP-dependent prote...

Ngày tải lên: 19/02/2014, 07:20

... 271) 705 encoding the DNA-binding domain of HSF, whereas clone C2 contained an insert of 2771 nucleotides including introns and an ORF encoding 513 amino acids. By using 5¢ -and3 ¢-RACE, the full-length ... (a 3 and b 3 ) and two heterotrimers (a 2 b 1 and a 1 b 2 ). The existence of the four types of trimer may be reflected in the broad band migrating at  200...

Ngày tải lên: 19/02/2014, 12:20

... vector following the manufacturer’s instruction (Gateway, Invi- trogen). The correct insertion of the Rv1399c ORF was confirmed by DNA sequencing. In our hands, protein expression using the commercially available ... Lac O (the DNA binding site of Lac I). The resulting construct constitutively expresses Lac I, which inhibits the T7 RNA polymerase in b inding to its specific...

Ngày tải lên: 07/03/2014, 16:20

... Li R, Hartley L & Robb L (2001) Cloning of rat inter- leukin 11 and interleukin 11 receptor alpha chain and analysis of their expression in rat uterus in the peri- implantation period. Reproduction ... (NQT) are in bold and underlined. The four potential poly(A) signals (AATAAA) in the 3¢-UTR and the TATA box in the 5¢-flanking region are boxed. T. Wang et...

Ngày tải lên: 07/03/2014, 16:20

... family and contains a di-zinc binding domain and a small domain that is inserted in the middle of the metal- binding domain and mediates catalysis. The di-zinc Fig. 6. Metal effects on the enzymatic ... downstream from His80 in the vicinity of the zinc center and is assumed to clamp the imidazolium ring of His80. Glu149 is in the immediate vicinity of th...

Ngày tải lên: 23/03/2014, 06:20

... We obtained a human and a murine NICN1 cDNA clone from the IMAGE collection and determined the complete sequences of these cDNAs. The comparative analysis of the human, canine, and murine NICN1 ... embryos. These experimentsshowed auniform staining of the mouse embryos (data not shown) indicating the presence of Nicn1 transcripts in all tissues of the developin...

Ngày tải lên: 23/03/2014, 21:20

... contribution of these elements to ZAS-N Znf binding, mutations were created in either the GAGA box or the trinucleotide CCC sequences in a 27-mer duplex spanning the protected region. The binding of the ... by the generation of deletion mutants containing discrete functional domains of XHIVEP1. Thus, the cloning and characterization of the XHIVEP interactin...

Ngày tải lên: 30/03/2014, 13:20

... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind- ing and uptake of these two isoforms does ... pulchellus seeds contain at least seven closely related and highly toxic type 2 ribosome-inactivating pulchellins, each consisting of a toxic A-chain linked to a sugar...

Ngày tải lên: 18/02/2014, 16:20

... coincide with the N-terminus of the intracellular enzyme (Fig. 1). The segments in the N-terminal portion around the copper ligand amino acids of the active site are well conserved between the ... J-C, Asther1 M & Lomascolo A (2006) Cloning and characterization of a tyrosinase gene from the white-rot fungus Pycnoporus sanguineus, and overproduction of th...

Ngày tải lên: 19/02/2014, 06:20