Báo cáo khoa học: Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase doc

... identified in the genome of C. difficile strain 630, which encoded both the glycyl radical subunit of the decarboxylase (HpdB) and its activating enzyme (HpdA). A detailed analysis of the sequence ... responsible for the change of the oligomeric state. These data point to an essential function of the small subunit of the decarboxylase and may indicate un...

Ngày tải lên: 07/03/2014, 15:20

... those of E. californicum allows the unambiguous assignment to distinct subunit types. The orthologous subunits of these species share 69.1–76.2% of their amino acids, with the a subunits being the ... a-subunits and the ancestor of all other subunit types. Further differentiation of subunit types occurred about 450–470 MYA, with the exception of the b/c-subunit...

Ngày tải lên: 08/03/2014, 08:20

... transcriptional regulation of the human S5a gene, we used PCR to isolate 1.2 kb of HeLa genomic DNA upstream of the first exon of the A B C D E F 24h 48h 72h 96h Regulation of subunit S5a of the 26S proteasome ... the N-terminus of S5a [26,27,29], which is highly conserved among all known S5a homologs. The N-terminus contains a VWA domain that is critical to the a...

Ngày tải lên: 23/03/2014, 09:20

... of the bacteria with the proteins, no reduction of the XTT was observed (data not shown). This suggests that the bacteria were now dead and also that the reduction of XTT was due to enzymes of ... The bars represent the mean percentage survival of two samples of bacteria and the error bars the SDs of these means. Percentage survival was calculated from the fr...

Ngày tải lên: 23/03/2014, 07:20

... the rate of the conformational change, k 3H the hydride transfer and k 4 the rate of the product-off step. Hence, in these circumstances, the steady-state K M is expected to be smaller than the apparent ... above, hence the tag did not influence the kin- etic behaviour of the enzyme to any measurable extent. Equilibrium binding affinity of NADH The binding of N...

Ngày tải lên: 23/03/2014, 09:20

... kinase (AK; EC 2.7.2.4) is an enzyme that catalyzes the first committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosynthetic pathway of the aspartic acid group amino ... subunits [4–6]. AK of the a 2 b 2 type is encoded by in-frame overlapping genes, so that the amino acid sequence of the b -subunit is identical to about 160 amino a...

Ngày tải lên: 18/02/2014, 08:20

... form of the PP2A holoenzyme is the trimeric A:B:C complex. The num- ber of B-type subunits is still growing with new members continuously being discovered. The subunit composition of the holoenzyme ... indicates the elution positions of molecular mass markers are shown above the panels. The profiles of the three proteins overlap over a region that coincides with...

Ngày tải lên: 07/03/2014, 05:20

... that the chan- ged conformation of PSI-F in the absence of PSI-J renders a fraction of the PSI complexes inactive. The in vivo measurements of the P700 redox level indicate that P700 in the DPSI-J ... as the second-order rate constant was reduced by 40% in the absence of PSI-N [7]. The increase in the pool of inactive PSI observed in plants devoid of PSI-J is...

Ngày tải lên: 16/03/2014, 11:20

... associa- tion of RIa and Cb1D4 because neither cAMP sensitiv- ity nor maximum activity of the endogenous PKA holoenzymes appeared to be affected by the relative high levels of transfected PKA subunits. The ... Autophosphorylation of the catalytic subunit of cAMP-dependent protein kinase. J Biol Chem 267, 25174–25180. 10 Yonemoto W, McGlone ML & Taylor SS (1993) N-myris...

Ngày tải lên: 30/03/2014, 04:20

... 2F9 are located on the same site but at the bottom part of the C-terminal domain. The only moderate effect of MalT on binding of 2F9 argues against these residues being part of the MalT–MalK interaction ... binding to the MalFGK 2 complex in the presence of MalT and enzyme IIA Glc , respectively, thereby providing the first direct evidence for the C-terminal domai...

Ngày tải lên: 31/03/2014, 09:20