Báo cáo khóa học: Chemical foundation of the attenuation of methylmercury(II) cytotoxicity by metallothioneins pptx

... indicative of the loss of Zn(SCys) 4 chromophores and thus of the removal of Zn(II) from the corresponding proteins. Although these two features, the common evolution of the three MTs and the loss of ... to the end of the titration, which is identified by the saturation of the CD features. This occurs for the addition of 16 MeHg(II) eq to the aMT fra...

Ngày tải lên: 07/03/2014, 15:20

... confirmed the active role of miR-23a in the growth promotion of malignant cells. The fundamental function of miRNAs is to regulate their targets by direct cleavage of mRNA or by inhibi- tion of protein ... with the first potential binding site of the IL6R mRNA 3¢ UTR. (A) The three EGFP reporter vectors bear- ing mutations of the miR-23a seed region binding site...

Ngày tải lên: 18/02/2014, 04:20

... accelerate the turnover of actin filaments by severing them [3–6], thereby increasing the number of the free pointed and barbed ends, or by increasing monomer dissociation from the pointed end of fila- ments ... speculate that the Pi release is promoted by opening of the ‘back door’ [38], while ADP becomes less accessible through the closing of the ‘main door’ on...

Ngày tải lên: 19/02/2014, 07:20

... may enter the citric acid cycle completing the catabolism of the pyrrolidine moiety of nicotine. Expression of the genes of these enzymes was dependent on the presence of nicotine in the growth ... expression of these genes depends on the presence of nicotine in the growth medium and we have determined the enzyme activities of the proteins. Our results demo...

Ngày tải lên: 19/02/2014, 07:20

... encoded in the nucleus, synthesized by cytosolic polysomes, and then imported into mitochondria, thereby reaching their final location in the inner membrane [17]. The similarities of the yeast ... around the catalytic core of the enzyme to arrive at the three dimen- sional organization revealed by the crystal structures (Fig. 1A). The supernumerary subunits and the...

Ngày tải lên: 19/02/2014, 12:20

... with the familiar pattern of expression of the principally embryonic cA–F genes in the rodent lens [41,47]. The absence of expression in outer layers of the lens contrasts with the expression of ... man [8,9]. In the verteb- rate lens, the b and c crystallins together account for the majority of the soluble proteins (the other major family being the a-crysta...

Ngày tải lên: 19/02/2014, 17:20

... the question arises concerning the mechanisms underlying the orchestrated expression of these enzymes by the oxygen tension. The findings that the effect of hypoxia on the gene expression of the ... of the procollagen hydroxylases. With the exception of PDI, which is known to be induced by the UPR [41], however, none of the other enzymes was relevantly i...

Ngày tải lên: 20/02/2014, 02:21

... also observed in the rate of degradation of the remaining mutant protein (20% left of the protein) vs. the wild type (40% left of the protein) at 3 h of chase (Fig. 7A,B). We also analyzed the rate of degradation of ... Ga q subunits. Therefore the lack of change in the rate of degradation cannot be due to lack of receptor-activa- tion of the alpha subu...

Ngày tải lên: 20/02/2014, 03:20

... is limited by the size of an intracellular chelatable Fe pool or permeability of the Fe–chelator complex. In view of the similar efficacy of the multidentate pyridinones to DFO, further experiments ... 10-week-old-male Wistar rats. The procedure was approved by the Animal Experimentation Ethics Committee of the University of Western Australia and is in accordance...

Ngày tải lên: 20/02/2014, 11:20

... study therefore, we investigated how O 2 modulates the expression of mRNAs for CytOX subunits and also the catalytic activity of the enzyme complex. Our results show that the levels of some of the ... membrane fragments (SMP) by the method of Smith [20], wherein the rate of oxidation of ferrocytochrome cwas measured by following the decrease in absorbency of...

Ngày tải lên: 20/02/2014, 23:20