Báo cáo khóa học: O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma docx

... questions concerning the occurrence and enzymatic processes involved in the O-acetylation and de -O-acetylation of sialic acids in human colonic tissues. Correspondence to J. Tiralongo, Institute for ... free sialic acids isolated from the mucosa of matched resection margin and colorectal carcinoma tissue. Sialic acids were isolated and analysed by HPLC a...

Ngày tải lên: 07/03/2014, 14:20

... flask-shaped invaginations of the plasma membrane and also by the presence of the structural protein caveolin (isoforms 1–3) [1]. Caveo- lin-1 and caveolin-2 are found in most cell types, inclu- ding ... Nystrom 3 and Peter Stra ˚ lfors 1 1 Department of Cell Biology and Diabetes Research Centre, University of Linko ¨ ping, Sweden 2 Department of Molecular and Clinical Me...

Ngày tải lên: 16/03/2014, 13:20

... the combination of amino acids at positions 3 and 6 might be a critical determinant for protein N- myristoylation. In the present study, to examine the effect of the combination of amino acids ... with serum-containing medium and incubated again at 37 °Cfor 24 h. The cells were then washed twice with 1 mL of serum- free DMEM and incubated for 5 h in 1 mL of DMEM with 2...

Ngày tải lên: 16/03/2014, 16:20

... transcribed regions of active genes in human T cells [124]. In a study in yeast, mutations were introduced alone or in combination in lysines 5, 8, 12 and 16 in the gene for histone H4 [125]. Of these, ... AcH4-K16 within transcribed genes is closely correlated with binding of both MOF and Tip60 (equivalent to Esa1 in yeast NuA4) [124,130]. However, in human cell...

Ngày tải lên: 14/02/2014, 18:20

... Further- more, in human breast tumors and cell lines, miR-17- 5p and miR-20a are induced in a manner that depends on cyclin D1 and repress the expression of cyclin D1. Hence, miR-17-5p ⁄ 20a and cyclin D1 ... repressing the indicated targets and presumably hundreds of other as yet unidentified targets. (B) miR-34a and the miR-34a-binding site in the 3¢ UTR of genes sh...

Ngày tải lên: 14/02/2014, 19:20

... upregulation of pro-IL-1b and that processing of pro-IL-1b into mature IL-1b requires activating cleavage of pro-caspase 1 [23], the cleavage of both pro-IL-1b and pro-caspase 1 was examined in hypox- ia ... Processing of pro-IL-1b into its active form AB CD Fig. 8. MSC-secreted IL-10 is involved in the inhibition of cardiac fibrosis. (A) BrdU incorporation in cardiac fibr...

Ngày tải lên: 18/02/2014, 04:20

... differently from the rest of the promoters. Of these, amino acid residues 243 and 275 are involved in DNA binding and 244 and 246 are in very close proximity to DNA binding. This indi- cates that p53R2 ... additional testing and usage of different training methods. We also developed similar prediction schemes for the remaining promoters and evaluated them in the same way...

Ngày tải lên: 18/02/2014, 11:20

... chains of ricin, a toxic protein inhibiting protein synthesis. Biochemistry 12, 3121–3126. 21 Olsnes S & Pihl A (1973) Isolation and properties of abrin: a toxic protein inhibiting protein ... suboptimal binding of GalNAc in one or both binding pockets of P I. However, in striking contrast with the rescue of protein synthesis observed for P I and P II, protection against...

Ngày tải lên: 18/02/2014, 16:20

... (PX) domain in the N-terminus. PX domains occur in a variety of proteins involved in cell signaling, mem- brane trafficking, and polarity establishment, and func- tion as phosphoinositide-binding modules ... in the testis and fetal brain. The Noxo1c protein contains an additional five amino acids in the N-terminal PX domain, a phosphoinosi- tide-binding module; the domain pl...

Ngày tải lên: 19/02/2014, 06:20

... Buchert 1 and Kristiina Kruus 1 1 VTT Technical Research Centre of Finland, Espoo, Finland 2 Protein Chemistry Research Group and Core Facility, Institute of Biotechnology, University of Helsinki, Finland Tyrosinase ... mutations and to localize the introns. The gene is interrupted by seven short introns. The encoded protein consists of 571 amino acids, including a predicted si...

Ngày tải lên: 19/02/2014, 06:20