Báo cáo khóa học: Structural properties of the protein SV-IV potx

... identified by the mass increase of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... electrospray MS. Theoretical mass is the mass calculated on the basis of the protein amino-acid sequence. The relative abundance refers to the ratio of th...

Ngày tải lên: 07/03/2014, 14:20

... wasusedasanegative control. The refolding of the scrambled RNase in the presence of Pf PDO seems to be less efficient when using PDI. However, the temperature of the assay, which is limited by the stability of the protein ... active sites. Refolding of the scrambled RNase in the presence o f P DI was used as a positive control, and the absence of the recover...

Ngày tải lên: 16/03/2014, 16:20

... [1]. The structural retroviral polyprotein (Gag) of HIV-1 forms the immature capsid, and is subse- quently cleaved by the viral protease into several mature proteins: the matrix, the capsid protein ... residues 146–231 of the intact protein; CACW40A, mutant of CAC with Ala instead of Trp at position 184 of CA; CSA, chemical shift anisotropy; Gag, the structural...

Ngày tải lên: 07/03/2014, 06:20

... likely due to an elongated shape of the protein. The same behavior is observed in the presence of GDPÆAlF x . Because of the unexpected observation of a monomeric protein species with both GDP and ... account). The size of the protein complex is only slightly increased when nucleotide-free or in the presence of GTP. As with hGBP5ta, the complex is increased to...

Ngày tải lên: 15/03/2014, 10:20

... Switzerland The fidelity of protein synthesis depends on the correct attachment of amino acids to the 3¢-ends of their cog- nate tRNA species by aminoacyl-tRNA synthetases (aaRS), a family of enzymes ... diminished Fig. 5. Model of the acceptor end of tRNA Ser bound in the active site of mMbSerRS. The view is focused on the acceptor part of tRNA in the active...

Ngày tải lên: 16/03/2014, 06:20

... study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP. ... structure of 282 residues of a total of 344 have been solved [19]. The catalytic domain of PKCh possesses a high degree of homology ( 80%) with the catalytic do...

Ngày tải lên: 16/03/2014, 13:20

... for the p eptide. NMR s pectroscopy was further utilized to explore the structural properties of Nogo-40. The very narrow reson- ance dispersion of amide protons ( 0.7 p.p.m) and the lack of ... Zn 2+ . Furthermore, the d etermination of the Nogo-40 solution structure offers a starting point for further understanding the interaction between NgR and Nogo-40, and for...

Ngày tải lên: 16/03/2014, 16:20

... of note that the structure of the LPS O-antigen of F. columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21], which is a linear polymer of ... and J 1,2 8.8 Hz. Thecorrelation of H2C tothe C2C at55.4 p.p.m. is consistent with the presence of a C2 acetamido substituent, and the lack of a proton at C6, considered...

Ngày tải lên: 17/03/2014, 10:20

... third expresses the untranslated 3¢ part of the mRNA [11]. Neither the primary structure, nor the repetitive ele- ments of the intact semenogelins are similar to motifs seen in other proteins, and thus the ... by exposure to Zn 2+ . The observation that high concentrations of urea dissolve the gel strengthens the assumption that the structure of SgI (as the domin...

Ngày tải lên: 23/03/2014, 07:20

... the rate of the conformational change, k 3H the hydride transfer and k 4 the rate of the product-off step. Hence, in these circumstances, the steady-state K M is expected to be smaller than the apparent ... of either substrate or cofactor with varied concentrations of the other. The experi- ments were performed at different, fixed concentrations of either lactate or...

Ngày tải lên: 23/03/2014, 09:20