Báo cáo khoa học: Crosstalk between Src and major vault protein in epidermal growth factor-dependent cell signalling docx

... regulatory and ⁄ or substrate proteins and are integrated into cell signalling pathways and cell cycle events [23]. The UD, Src homology 3 and Src homology 2 (SH2) domains in Src are key binding sites ... a Src SH2 binding protein. We observed that MVP interacts with Src in mammalian cells and inhibits the activity of Src tyrosine kinase. Results Isolation of M...

Ngày tải lên: 07/03/2014, 12:20

... of proteins including b-synuclein and c-synuc- lein [3]. In contrast to a-synuclein, these two proteins do not appear to aggregate or form fibrils [26]. How- ever, b-synuclein is known to inhibit ... whether metal binding to a-synuc- lein is an artefact or not. However, the new evidence suggesting that a-synuclein is a metal binding protein is intriguing and is likely to result in a...

Ngày tải lên: 07/03/2014, 10:20

... its C-terminus. The mature cPDI protein has a calculated molecular mass of 54 913.7 Da and an isoelectric point of 4.6. The cPDI contains four thioredoxin domains and an acidic C-ter- minal tail ... conotoxins have been thoroughly investigated, and two distinct folding mechanisms have been unveiled [20]. Through comparison of folding kinetics and thermodynamics, the folding mechanis...

Ngày tải lên: 07/03/2014, 05:20

... most 5% of the traffic, with the majority of traffic pass- ing through the main entrance. In mutants Trp83Ala and Trp83Glu, ligands that close the main entrance do not inhibit substrate hydrolysis because ... DmAChE (residues and labels in green) and Torpedo californica acetylcholin- esterase (residues and labels in fushia). Residues are represented by sticks. The hydrogen bonds i...

Ngày tải lên: 07/03/2014, 05:20

... containing CLP and CLAP with a phosphoprotein stain did not reveal phosphate additions to these proteins. Only lanes in the gel containing the known phosphoproteins ovalbumin, b-casein and pepsin ... fasciclin domains in CLAP with other fasciclin I containing proteins is clear (Fig. 9). Using BLAST (http://www.ncbi.nlm.nih.gov/BLAST) to identify related proteins, a putative cell adh...

Ngày tải lên: 07/03/2014, 16:20

... structural and func- tional determinants of the protein protein interaction between v-KIND and MAP2 (i.e. the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across ... with GST-CD2 (MAP2 v-KIND binding core). Input (cerebellum) GST v-KIND KIND1 v-KIND KIND2 Spir-2 KIND PTPN13 KIND Input (MAP2) GST v-KIND KIND1 v-KIND KIND2 Spir-2 KIND PTPN13 KIND...

Ngày tải lên: 14/02/2014, 19:20

... Relationship between BMI, total testosterone, sex hormone-binding-globulin, leptin, insulin and insulin resistance in obese men. Arch Androl 52, 355–361. 19 Wannamethee SG, Shaper AG, Morris RW & Whincup ... the production of in ammatory cytokines (e.g. IL-6, TNFa) as well as lipoprotein lipase, angiotensinogen, free fatty acids, resistin, leptin, lactate, PAI-1, insulin and adip...

Ngày tải lên: 18/02/2014, 06:20

... loops and occur at constant activities of Src inhibitors and activators, such as C-terminal Src kinase and receptor- type protein tyrosine phosphatases. We demonstrate that C-terminal Src kinase and ... fibronec- tin-coated beads adhering to the cells. As fibroneciton binds to integrins, the local pulling force stimulated integrins that subsequently activated Src. Intriguingly,...

Ngày tải lên: 18/02/2014, 11:20

... the cells was determined. The cells included the K562 cell line and its Pgp over-expressing subline (K562 ⁄ ADR), and the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ADR). Innate ... cells was determined as described in Fig. 1. The cells included the K562 cell line and its Pgp over-expressing subline (K562 ⁄ ADR), the GLC4 cell line and its MRP1 over-expres...

Ngày tải lên: 18/02/2014, 13:20

... (1996) Activator anion binding site in pyridoxal phosphorylase b: The bind ing of phosp hite, phosph ate, and fluorophosphate in the c rystal. Protein Sci. 5, 2416–2428. 26. Schinzel, R., Palm, D. ... Enzyme–oxyanion interactions occur a t a protein site different from the active site, and thermostabilization is the result of a protein conformational change induced by the binding...

Ngày tải lên: 19/02/2014, 16:20