Báo cáo khoa học: Limited mutagenesis increases the stability of human carboxypeptidase U (TAFIa) and demonstrates the importance of CPU stability over proCPU concentration in down-regulating fibrinolysis doc

... molecular link between coagulation and fibrinolysis. Upon activation of proCPU, the active enzyme (CPU) rapidly becomes inactive due to its intrinsic instability. The inherent instability of CPU ... But the instability of the enzyme has prevented crystallization of CPU and the use of struc- turally based drug design methods. A three-dimen- sional model of hum...

Ngày tải lên: 07/03/2014, 12:20

... IFN-b through the phosphorylation of multiple cellular factors, including IFN regulatory fac- tor-3 (IRF-3) and kinases, including the Tank-binding kinase 1 (TBK-1) and inhibitor of j B kinase e ... expression of the IRF-3 ORF. The obtained DNA fragment was subcloned into the XhoI and HindIII sites of pEGFP-C1 (Clontech, Mountain View, CA, USA), and the obtained pE...

Ngày tải lên: 18/02/2014, 16:20

... toxin A chain without the corresponding B subunit, remains uncertain because its intracellular transport route bypasses the Golgi apparatus [9]. In this work, our studies to delineate the route ... between the extent of lipopolyamine-induced sensi- tization to saporin versus free ricin A chain. Thus, as with the extent of dimethylsulfoxide-induced toxin sen- sitization and...

Ngày tải lên: 07/03/2014, 05:20

... acknowledge the risk of influ- ential interaction between the interviewer and the inter- viewed during the interviews that could influence the statements of the interviewed e.g., the use of leading ques- tions. In ... analysis of the veterinarians' perceptions of how they wished to use the metritis score in their practice and on dialogue with the far...

Ngày tải lên: 25/10/2012, 10:45

... 3B). To investigate the individual roles CSD and PTB proteins may play in directing CCV1 complex binding to the 5¢-ACCUCUU-3¢ and 5¢-UUUUCUU-3¢ sequences, recombinant CSD (GST-dbpB/YB-1) and PTB ... 5¢-UUUUCUU-3¢.5¢-UCUU-3¢ sequences flanked by pyrimidine residues are commonly found in PTB binding sites [52–55,57]. Mutation of either sequence, by a block mutation (V37, V39) or...

Ngày tải lên: 19/02/2014, 12:20

... by the 5-fluoro substituents and to contacts lost to Ile50/Ile50¢ by the 2-fluoro substituents. Hence, t he K i values are in uenced not only by the number of fluorine substituents but also by the ... is possible that the presence of two binding configurations of the fluoro-substituted benzyloxy side groups in the S1/S1¢ subsites may account for the general reduction in...

Ngày tải lên: 19/02/2014, 16:20

... level in almost all tissues. These data are in agreement with those of Pedersen et al. [3], who found the mRNA of the murine homologue SAM9 mainly in brain, but also in other tissues. In situ mRNA ... showed a neuronal expression in the adult and developing murine brain [3]. The human multiple- tissue expression array shows a weak signal for Hu-K4 in the corpus...

Ngày tải lên: 19/02/2014, 17:20

... subunits of PDH1, PDH2 and T. profundus dye-l-proDH. The amino-acid sequence of the a1 subunit of PDH1 showed 31% and 32% identi- ties with the a2 subunit of PDH2 and the a subunit of the T. profundus ... while the b1 subunit showed 56% and 64% sequence identities with the b2 subunit of PDH2 and the b subunit of the T. profundus enzyme, respectively (...

Ngày tải lên: 20/02/2014, 01:20

... homology domains such as the GLUE (GRAM-like ubiquitin-binding in EAP45) domain [32–34]. Intracellular localization of PtdIns3P The identification of PtdIns3P-binding domains offered the possibility ... contains as many as five ubiquitin-binding domains, serves to sequester ubiquitinated cargoes, whereas ESCRT-I and -II, which also contain ubiquitin-binding domains, serve to form...

Ngày tải lên: 06/03/2014, 01:23

... receptors involved in the rec- ognition and killing of tumors, including clinically important solid tumors. In view of all these results, the availability of long-circulating and in vivo stable solu- ble ... of the kinetics of accumulation of soluble CD69 receptors in the liver and kidney indicates a fast uptake of the proteins with a short half-life in...

Ngày tải lên: 16/03/2014, 04:20