Báo cáo khoa học: Induction of PPARb and prostacyclin (PGI2) synthesis by Raf signaling: failure of PGI2 to activate PPARb potx

... highly potent PPARb agonists, if exist- ent, remain to be identified. Results Induction of prostanoid synthesis by C -Raf signaling To investigate the effect of Raf signaling on prostanoid synthesis ... the dramatic induction of PGE 2 and PGI 2 synthesis after Raf activation. The strong induction of Cox-2 expres- sion was virtually abolished by both the ERK...

Ngày tải lên: 07/03/2014, 12:20

... cova- lently attached to the C-termini of certain secretory proteins and act as anchors to attach such proteins to the outer face of the plasma membrane [1,2]. Synthesis of GPIs is essential for ... mannose (Man3) of all yeast GPIs [9] and of certain human GPIs [10–12], and additional side-branching phospho- ethanolamines (EthN-Ps) may be added to the first and seco...

Ngày tải lên: 07/03/2014, 16:20

... (AA) is first converted into prostaglandin G 2 (PGG 2 , catalytic step 1), and then to prostaglandin endoperoxide [prostaglandin H 2 (PGH 2 )] (catalytic step 2) by COX isoform-1 (COX-1) or COX-2 ... downstream synthases, and is isom- erized to prostaglandin D 2 , prostaglandin E 2 (PGE 2 ), prostaglandin F 2 (PGF 2 ), and prostaglandin I 2 (PGI 2 ) or thromboxane A 2 (TXA 2 ) by...

Ngày tải lên: 30/03/2014, 02:20

... suppressed by the Src-specific inhibitor CGP77675 as well as by the MEK1 inhibitor UO126 and partially attenuated by the PI3K inhibitor Wort- mannin. No effect was observed with inhibitors of p38 MAPK, ... addi- tion to the loss of cell–cell adhesion, involves degrada- tion of the extracellular matrix and release of cells from the constraints of cell–cell and cell–matri...

Ngày tải lên: 19/02/2014, 05:20

... FEBS 1803 Induction of raft-like domains by a myristoylated NAP-22 peptide and its Tyr mutant Raquel F. Epand 1 , Brian G. Sayer 2 and Richard M. Epand 1,2 1 Department of Biochemistry and Biomedical ... stream of nitrogen with constant rotation of a test tube to avoid separation of lipid components [12] and to deposit a uniform film of lipid over the bottom third...

Ngày tải lên: 07/03/2014, 17:20

... stimulation of TCTP synthesis by copper was mediated by the TCTP mRNA 3¢-UTR (3.2-fold) but not by the 5¢-UTR (0.5-fold). mRNA stabilization was found to mediate these effects of cobalt and nickel. ... does not differ as compared to the control; (c) dioxin-, nickel- and cobalt-treated extracts show qualitative (appearance of new bands and suppression of control bands...

Ngày tải lên: 16/03/2014, 05:20

... regulatory step of glycolysis catalyzed by phosphofructokinase, which is used by glucose, but not fructose. This rapidly activates AMPK rather than inhibiting AMPK as glucose does. Therefore, fructose and ... entry of fatty acid into mitochon- dria. Thus, the steady-state level of malonyl-CoA in these tissues is determined by the relative activities of ACC and malonyl-CoA de...

Ngày tải lên: 14/02/2014, 22:20

... hydrogen-bonded via one water molecule to the O3 atom of pantoate, and via two water molecules to the O4 atom of panto- ate. The Ne atom of His37 is hydrogen-bonded to the O4 atom of pantoate via a water molecule, ... in turn hydrogen-bonded to a network of water molecules that are hydrogen-bonded to the O1 atom of pantoate in site I. The O4 atom of pantoate in site...

Ngày tải lên: 16/02/2014, 09:20

... of endothelial cells and macrophages, as well as the differentiation and proliferation of VSMCs. Overexpression of KLF4 induced expression of multiple anti-inflammatory and antithrombotic factors, ... surface and prolongation of clotting time following the induc- tion of KLF4 under inflammatory states, and implicat- ing KLF4 as a regulator of endothelial activation in re...

Ngày tải lên: 18/02/2014, 04:20

... activity, inhibitor reactivity and sTF binding of G372A-FVIIa The very slow auto-activation of G372A-FVII, or rather the need to add factor IXa for activation to occur, was the first sign of the relatively ... loop and with activation loop 2, by studying the effects of replacement with Ala [G372(223)A]. G372A-FVIIa, both in the free and TF-bound form, exhibited reduced clea...

Ngày tải lên: 18/02/2014, 08:20