Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas fluorescens potx

... with the gene of the benzaldehyde lyase and for helpful discus- sions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their help with data collection and ... Journal compilation ª 2005 FEBS Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas fluorescens Tanja G. Mosbacher...

Ngày tải lên: 07/03/2014, 12:20

... field [32]. The final 16 lowest energy structures of DMT1-TM4 in both SDS (pH 6.0) and TFE were chosen to represent the solution structures of the peptide, as shown in Fig. 3. The quality of the final structures ... shifts for DMT1-TM4 in TFE and SDS. Ó FEBS 2004 Structure and topology of TM4 of DMT1 (Eur. J. Biochem. 271) 1951 Structure and topology of the tr...

Ngày tải lên: 16/03/2014, 16:20

... than the isofunctional enzymes from other bacteria (Fig. 2). Expression of ArCMLE1 Comparison of the sequence of ArCMLE1 with that of the recently published crystal structure of the CMLE from ... in the mutant. First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structure o...

Ngày tải lên: 30/03/2014, 10:20

... with the uncomplexed protein. Com- parisons of the structure of Rio1 with the previously determined structure of the Rio2 kinase defined the minimal RIO domain and the distinct fea- tures of the ... Asp99 and Phe102, Met104 and Ile107, and Ser108 and Glu111, which marks the start of aC. The entire flexible loop packs between the N-terminal portion of...

Ngày tải lên: 30/03/2014, 20:20

... C (1989) The limits of the DNase I-sensitive domain of the human apolipoprotein B gene coincide with the locations of chromosomal anchorage loops and define the 5¢ and 3¢ boundaries of the gene. ... Since then there has been an explo- sion of papers on the multitude of chromatin modifica- tions and the factors that can either create or recognize them. We now ha...

Ngày tải lên: 14/02/2014, 18:20

... N-terminal of the mature enzyme and by the ÔflapÕ. The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionic interactions established between Lys11/Tyr13 of ... hybridization, in the digestive glands of the pitchers, the trapping organs of the plant [6]. Using immunohistochemistry and immunogold transmission...

Ngày tải lên: 19/02/2014, 12:20

... in which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja- cent and antiparallel to the b1-strand (Fig. 1). The length and sequence of the variable ... data from other KH domains to the KH1–KH2 domains of FMRP. The structure of the tandem KH1–KH2 domains of hFMRP provided the first crystallographic descriptio...

Ngày tải lên: 07/03/2014, 05:20

... comparable to the hexokinase subunits [24]. One lobe consists of the phosphate1 subdomain and the predicted a-helix I. The other lobe consists of the phos- phate2 and the adenosine subdomains, the predicted ... fitted into the glucokinase model using RasMol software. Figure 5 shows the predicted structure of GlcNAc kinase with the localiza- tion of the five A...

Ngày tải lên: 08/03/2014, 10:20

... estimation of the hydrodynamic radii of HAMLET (R h = 26.9 A ˚ ), which is intermediate of the hydrodynamic radii of the acidic molten globule state of a-lactalbumin (R h = 20.9 A ˚ ) and the theoretically ... fully understood. The active group of the matrix, the DEAE group, is posi- tively charged and might therefore bind negative mole- cules. At pH 8.5 (the pH...

Ngày tải lên: 29/03/2014, 21:20

... Among the 2314 mutants, 1148 were localized in the central core domain of the protein and were used for training and evalua- tion of our prediction algorithm. Of the eight promoters, we studied the ... prediction of the severity of p53 mutants. The method was trained on a set of known mutants and subsequently evaluated on another set. The method is based on p...

Ngày tải lên: 18/02/2014, 11:20