Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx
Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx
... 273 (2006) 2127–2138 ª 2006 The Authors Journal compilation ª 2006 FEBS
The guanine nucleotide exchange factor RasGRF1 directly
binds microtubules via DHPH2-mediated interaction
Greta Forlani, ... neither the isolated DH nor the
PH2 domain bound microtubules. In the absence of
microtubules, none of the recombinant proteins was
found in the pellet fraction...
Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt
... 270) 4543
the nucleotide exchange during the peptide translocation
process.
The nonreciprocal behaviour of the chimeric TAP-NBDs
suggests that the C-terminal regulation of nucleotide
interaction ... model for the transport cycle of the active TAP variant 2–1C2. The initiation step and the progress of the transport cycle are
similar to the hypothesized peptide tra...
Báo cáo khoa học: The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba docx
... due to the ingestion of Ginkgo remedies. However,
they neither specify the respective remedies, nor do
they mention their composition. Therefore, the possi-
bilities of overdosage or interactions ... the antivitamin character of both compounds
and explains a depletion of cofactor formation in the
organism in the presence of the antivitamins.
The kinetic data (Table 1) are a re...
Báo cáo khoa học: The role of interface framework residues in determining antibody VH ⁄ VL interaction strength and antigen-binding affinity pptx
... absorbance, others showed a
decreased or diminished antigen-dependency. To evalu-
ate the OS-fitness of the clone, the ratio of the specific
absorbance in the presence of 10 lgÆmL
)1
HEL to that
in the ... V
L
interaction strength and the
OS-fitness. When H39 was Lys (K), as in HyHEL-10,
the V
H
⁄ V
L
interaction in the absence of antigen was
generally weak, while the OS-...
Báo cáo khoa học: The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism docx
... Aminogluthimide inhibition showed that the three major
metabolites, but not the others, resulted from P450scc action. It therefore
appears that non-P450scc enzymes present in the adrenal cortex to some
extent ... tools for the characterization of secoster-
oid products. Furthermore, we tested vitamin D3 bio-
transformation by isolated mitrochondria from the
adrenal gland, the tiss...
Báo cáo khoa học: The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs pdf
... Taken together,
these results show that the ROQ domain is essential
for Roquin’s repression of Icos mRNA.
Roquin binds Icos mRNA
To determine whether Roquin directly binds the por-
tion of the Icos ... indi-
cated by a wedge above the relevant lanes, and the absence of
any added protein is indicated by ()). The position of the free RNA
and of the loading slots is indicat...
Báo cáo khoa học: The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed docx
... genes with the human cathepsin D gene. The top line of the figure shows the arrangement
of the functional regions of the plant aspartic proteinase genes like in Fig. 1. Below that are the representative ... four
times stronger in the tissue from the light when compared to
thetissuefromthedark.Thiswasthesameorderof
induction detected by the whole cDNA with the same RNA
(2J...
Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... activating a guanine nucleotide
exchange factor with activity towards Ras.
Results
HEK293 cells express the guanine nucleotide
exchange factor Ras-GRF1
The guanine nucleotide exchange factor Ras-GRF1 ... N-terminal deletions of the full-
length 140 kDa protein. The physiological role of the
guanine nucleotide exchange activity of the truncated
forms i...
Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc
... compared the results
with the rates of the i sotope exchange under t he same
conditions. We h ave found that in the
L
-phenylalanine
reaction the exchange o f t he abstracted proton in the active
site ... in the
active site; (b) the statistical factor taking account of the
ratio of protons and deuterons on the considered group
when the latter is polyprotic; (c) th...
Báo cáo khoa học: The propagation of hamster-adapted scrapie PrPSc can be enhanced by reduced pyridine nucleotide in vitro pdf
... Calculat-
ing the signal intensity of the reactions of 0 and
10 lm NADPH revealed that the efficiency of PMCA
was increased by 2.59-fold in the presence of
NADPH. To evaluate whether the effect ... required
for the same amplification.
PrP
Sc
generated from PMCA in the presence of
NADPH had further propagation ability
To determine whether newly generated PMCA PrP
Sc
by addition o...