Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx

... 273 (2006) 2127–2138 ª 2006 The Authors Journal compilation ª 2006 FEBS The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction Greta Forlani, ... neither the isolated DH nor the PH2 domain bound microtubules. In the absence of microtubules, none of the recombinant proteins was found in the pellet fraction...

Ngày tải lên: 07/03/2014, 12:20

... 270) 4543 the nucleotide exchange during the peptide translocation process. The nonreciprocal behaviour of the chimeric TAP-NBDs suggests that the C-terminal regulation of nucleotide interaction ... model for the transport cycle of the active TAP variant 2–1C2. The initiation step and the progress of the transport cycle are similar to the hypothesized peptide tra...

Ngày tải lên: 20/02/2014, 02:21

... due to the ingestion of Ginkgo remedies. However, they neither specify the respective remedies, nor do they mention their composition. Therefore, the possi- bilities of overdosage or interactions ... the antivitamin character of both compounds and explains a depletion of cofactor formation in the organism in the presence of the antivitamins. The kinetic data (Table 1) are a re...

Ngày tải lên: 07/03/2014, 10:20

... absorbance, others showed a decreased or diminished antigen-dependency. To evalu- ate the OS-fitness of the clone, the ratio of the specific absorbance in the presence of 10 lgÆmL )1 HEL to that in the ... V L interaction strength and the OS-fitness. When H39 was Lys (K), as in HyHEL-10, the V H ⁄ V L interaction in the absence of antigen was generally weak, while the OS-...

Ngày tải lên: 07/03/2014, 12:20

... Aminogluthimide inhibition showed that the three major metabolites, but not the others, resulted from P450scc action. It therefore appears that non-P450scc enzymes present in the adrenal cortex to some extent ... tools for the characterization of secoster- oid products. Furthermore, we tested vitamin D3 bio- transformation by isolated mitrochondria from the adrenal gland, the tiss...

Ngày tải lên: 07/03/2014, 21:20

... Taken together, these results show that the ROQ domain is essential for Roquin’s repression of Icos mRNA. Roquin binds Icos mRNA To determine whether Roquin directly binds the por- tion of the Icos ... indi- cated by a wedge above the relevant lanes, and the absence of any added protein is indicated by ()). The position of the free RNA and of the loading slots is indicat...

Ngày tải lên: 15/03/2014, 11:20

... genes with the human cathepsin D gene. The top line of the figure shows the arrangement of the functional regions of the plant aspartic proteinase genes like in Fig. 1. Below that are the representative ... four times stronger in the tissue from the light when compared to thetissuefromthedark.Thiswasthesameorderof induction detected by the whole cDNA with the same RNA (2J...

Ngày tải lên: 17/03/2014, 11:20

... activating a guanine nucleotide exchange factor with activity towards Ras. Results HEK293 cells express the guanine nucleotide exchange factor Ras-GRF1 The guanine nucleotide exchange factor Ras-GRF1 ... N-terminal deletions of the full- length 140 kDa protein. The physiological role of the guanine nucleotide exchange activity of the truncated forms i...

Ngày tải lên: 19/02/2014, 18:20

... compared the results with the rates of the i sotope exchange under t he same conditions. We h ave found that in the L -phenylalanine reaction the exchange o f t he abstracted proton in the active site ... in the active site; (b) the statistical factor taking account of the ratio of protons and deuterons on the considered group when the latter is polyprotic; (c) th...

Ngày tải lên: 19/02/2014, 16:20

... Calculat- ing the signal intensity of the reactions of 0 and 10 lm NADPH revealed that the efficiency of PMCA was increased by  2.59-fold in the presence of NADPH. To evaluate whether the effect ... required for the same amplification. PrP Sc generated from PMCA in the presence of NADPH had further propagation ability To determine whether newly generated PMCA PrP Sc by addition o...

Ngày tải lên: 07/03/2014, 03:20