Báo cáo khoa học: Insulin induces heme oxygenase-1 through the phosphatidylinositol 3-kinase/Akt pathway and the Nrf2 transcription factor in renal cells pptx

... of MEs and the potential outcome of intercepted and non-intercepted errors. Materials and methods In April 2002, University College Hospitals London ICU intro- duced the QS 5.6 Clinical Information ... writing process resulted in either an unin- tentional significant reduction in the probability of treatment being timely and effective or an unintentional significant increa...

Ngày tải lên: 25/10/2012, 10:39

... either the 4A- or the 7A-cotransfected cells, thus demonstrating that strong in vivo binding of the destabilized 13R4-d1-EGFP pro- teins allows them to be maintained in the cells. To rule out the ... mole- cules and the b-gal were strictly colocalized, it seems that the scFvs fused to d1-EGFP are maintained in the cells by binding to antigen. Generation of soluble...

Ngày tải lên: 19/02/2014, 18:20

... there is little direct evidence in the literature to confirm this link [21]. In insects, there has been a long-standing focus on Hsp70, which remains the most commonly studied stress protein in ... crucial role in determining the distribution and abundance of animals. In insects and other ectotherms, temperature simultaneously affects physiological processes, biophysical st...

Ngày tải lên: 06/03/2014, 09:22

... were discovered using high-throughput in vitro screening. Binding to the sub- strate-binding sites S1 and S3 is confirmed by intermolecular NOEs and comparison with the binding mode of a previously ... reported in the absence of inhibitor [4] and in the presence of peptide inhibitors [5,6] or bovine pancre- atic trypsin inhibitor (BPTI) [4]. In the absence of inhibitor,...

Ngày tải lên: 07/03/2014, 02:20

... 21 GSK3 and GSK3 a ⁄ b in N2a cells treated with 100 n M BBG for 30 and 60 min. Actin was used as a loading control and the graph represents the mean ± stan- dard deviation of the pGSK3 ⁄ actin ratio ... prominent in N2a cells [70]. The lack of CTP-mediated calcium responses also excludes the presence of functional P2X4 receptors in these cells [41]. In contrast,...

Ngày tải lên: 07/03/2014, 02:20

... terminal cys- teine with histidine in the metallothionein a and b domain maintains its binding capacity. Eur J Biochem 259, 519–527. R. Bofill et al. C. elegans CeMT1 and CeMT2 metallothioneins FEBS ... P17511) and CeMT2 (Uniprot P17512). The coordinat- ing and putative coordinating residues are highlighted (Cys in grey shadow, His and Tyr in bold) and the total content...

Ngày tải lên: 07/03/2014, 02:20

... with IGF-1 signaling also in C2 myoblasts overexpressing the insulin- like growth factor 1 binding protein 5 and therefore not responding to endogenously produced insulin- like growth factor 1. Although ... IGFBP5, insulin- like growth factor 1-binding protein; IRS-1, insulin receptor substrate 1; kiAKT, kinase-inactive form of AKT; LY, LY294002; Neu2, neuraminidase 2; HS, ho...

Ngày tải lên: 19/02/2014, 06:20

... proteinase K. DPP IV levels following stimulation with insulin and bpV(phen) In order to examine changes in DPP IV levels follow- ing insulin stimulation, rats were injected with the PTP inhibitor ... PTP inhibitor bpV(phen) 16 h and 30 min prior to insulin injection and isolation of the G ⁄ E and PM fractions. No effect on DPP IV level was detected following insulin i...

Ngày tải lên: 19/02/2014, 07:20

... tyrosine kinase activity in their intracellular domain [1]. The signalling pathway of insulin is similar to that of some other growth factors [2], and the key signalling molecules of insulin are phosphatidylinositide-3-kinase ... are phosphatidylinositide-3-kinase (PI-3K), phosphotidylino- sitide dependent kinase-1 (PDK-1) and protein kinase B (PKB, also called Akt) [3,4]. Whe...

Ngày tải lên: 21/02/2014, 00:20

... proteins con- tain heme o and heme a. Assuming similar extinction coefficients in acetonitrile for the Soret for both heme o and heme a, we estimate  25% heme a and 75% heme o for Surf1c W200F, and ... to heme binding in either bacterial version of the Surf1 protein, with a strategic tryptophan residue possibly interacting with the formyl group of heme a. Res...

Ngày tải lên: 06/03/2014, 00:21