Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

... Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells Monica Averna, Roberto Stifanese, Roberta ... clearly indi- cates that the ligand responsible for the changes in intracellular calpain conformation is its natural inhib- itor, calpastatin. The new conformational state acquired by calpain i...

Ngày tải lên: 07/03/2014, 12:20

... end for Figure 2: Algorithm for training Bayesian Networks for inference of lexical semantic roles After the training phase, a testing procedure using the Markov Chain Monte Carlo (MCMC) inference engine ... usually used as gold standards for training and evaluation of such systems. In the meantime, various existing parsing systems are also adapted to provide semantic information...

Ngày tải lên: 08/03/2014, 01:20

... were performed using the following reaction conditions: Initial incubation of 95 °C for 3 min followed by 45 cycles of 95 °C for 10 s, 60 °C for 20 s, and 72 °C for 30 s. A fluorescence reading determined ... activating protein Dab2IP in mouse brain. Mol Brain Res 115, 121–129. 56 Piddini E, Schmid JA, Martin R & Dotti CG (2001) The Ras-like GTPase Gem is involved in cell sha...

Ngày tải lên: 30/03/2014, 03:20

... structural and func- tional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e. the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across ... stain) EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 KIND2-1 487 KIND2-2 521 KIND2-3 555 KIND2-5 589 KIND2-4 488 456 620 KIND2 v-KIND +...

Ngày tải lên: 14/02/2014, 19:20

... CcCac1L. (C) Interaction between CcCac1L and CcLim15 in a yeast two-hybrid assay. The inserts in the activation domain (AD) and DNA-binding domain (BK) are shown. +, binding; ), no binding. The mild ... accumu- lating evidence regarding the role of CAF-1 in chroma- tin assembly following various DNA synthesis events, its involvement in chromatin assembly following mei- oti...

Ngày tải lên: 07/03/2014, 05:20

... MBD1 gene was induced by interferon, indicating that these genes are co-ordinately regulated. The interaction was confirmed in vitro and in vivo and was mapped to the ubiquitin-like domain of p59 ... LexA gene and the human p59 OASL. To identify proteins that interact with specific domains of p59 OASL, deletion mutants containing the P-loop, ATP-binding, and UbL domains, individua...

Ngày tải lên: 30/03/2014, 13:20

... the cells was determined. The cells included the K562 cell line and its Pgp over-expressing subline (K562 ⁄ ADR), and the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ADR). Innate ... over-expressing subline (K562 ⁄ ADR), the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ADR), the 2008 cell line and its sublines over-expressing MRP1, MRP2 or...

Ngày tải lên: 18/02/2014, 13:20

... cofactor, and the K m and k cat for AdoCbi-GDP were determined in the presence of 20, 70, 100, 150 and 200 lm cofactor. The reaction was initiated by adding l-glutamate and incubating at room ... containing 50% CH 3 CN. The flow rate was 1 mLÆmin )1 . The following profile was used for separation: 2 min isocratic development with 98% A; 5 min linear gradient from 98% A to 75% A; 1...

Ngày tải lên: 07/03/2014, 04:20

... described in the text. After removal of unbound cobalamin at 0 min (thick solid line), 30 min (thin solid line), 60 min (thin long-dashed line), 120 min (thin short-dashed line), 240 min (thin dotted ... damaged cofactors) for free adeninylpentylcobalamin (AdePeCbl) (an inactive coenzyme analog containing the adenine ring in the upper axial ligand; a model of intact coen- zyme, AdoCbl...

Ngày tải lên: 15/02/2014, 01:20

... information The following supporting information is available: Fig. S1. LPS induces the expression of ICAM-1 mRNA in cultured Schwann cells. This supporting information can be found in the online version ... lgÆmL )1 aprotinin and 1 lgÆmL )1 leupeptin], and clarified by centrifuging at 12 000 g for 20 min in a microcentrifuge at 4 °C. The protein concentration of the resulting su...

Ngày tải lên: 18/02/2014, 18:20