Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx
Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx
... Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells Monica Averna, Roberto Stifanese, Roberta ... clearly indi-cates that the ligand responsible for the changes in intracellular calpain conformation is its natural inhib-itor, calpastatin. The new conformational state acquired by calpain in these ... digest-ible protein substrate, and finally calpastatin. The finding that calpastatin was the most efficientligand at promoting calpain transition, detected as anincrease in mAb that bound to calpain, is...
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Báo cáo khoa học: "Mapping between Compositional Semantic Representations and Lexical Semantic Resources: Towards Accurate Deep Semantic Parsing" docx
... end for Figure 2: Algorithm for training Bayesian Networks for inference of lexical semantic rolesAfter the training phase, a testing procedure usingthe Markov Chain Monte Carlo (MCMC) inferenceengine ... usually used as gold standards for training and evaluation of such systems. In themeantime, various existing parsing systems are alsoadapted to provide semantic information in their out-puts. ... the corresponding VerbNetclass.5: Initialize edges connecting corresponding features.6: Append the current features as evidence for the network.7: end for 8: Start Training Model for the current...
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Báo cáo khoa học: Effect of ecdysone receptor gene switch ligands on endogenous gene expression in 293 cells docx
... were performed using the followingreaction conditions: Initial incubation of 95 °C for 3 minfollowed by 45 cycles of 95 °C for 10 s, 60 °C for 20 s, and 72 °C for 30 s. A fluorescence reading determined ... activating protein Dab2IP in mouse brain. MolBrain Res 115, 121–129.56 Piddini E, Schmid JA, Martin R & Dotti CG (2001)The Ras-like GTPase Gem is involved in cell shaperemodeling and interacts ... levels are induced byproinflammatory cytokines in PHM1-31 myometrial cells and that MAF transcription factor mRNA israpidly induced by IL-1B and TNF in primarymyometrial and PHM1-13 cells. Our...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... structural and func-tional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e. the MAP2-bindingcore module in v-KIND and the v-KIND-binding coremodule in MAP2) across ... stain)EGFPEGFP-KIND2-1EGFP-KIND2-2EGFP-KIND2-3EGFP-KIND2-4EGFP-KIND2-5EGFP-KIND2EGFP-KIND1KIND2-1487KIND2-2521KIND2-3555KIND2-5589KIND2-4488456 620KIND2v-KIND+++–– Interaction EGFPEGFP-KIND2-1InputIB: ... sufficient for thev-KIND-MAP2 interaction within cells. The MAP2-binding core module of v-KIND isinvolved in targeting to neuronal dendrites and dendrite growthWe next investigated the MAP2 binding...
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Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot
... CcCac1L. (C) Interaction between CcCac1L and CcLim15 in a yeast two-hybrid assay. The inserts in the activation domain(AD) and DNA-binding domain (BK) are shown. +, binding; ), no binding. The mild ... accumu-lating evidence regarding the role of CAF-1 in chroma-tin assembly following various DNA synthesis events, its involvement in chromatin assembly following mei-otic recombination is unknown. In ... cloning of CcCac1L and its interaction with CcLim15. (A) Schematic diagram of the CAF-1 large subunits in human, C. cine-rea and S. cerevisiae. The KER and ED domains are represented by black and...
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Báo cáo khóa học: Interaction between the 2¢)5¢ oligoadenylate synthetase-like protein p59 OASL and the transcriptional repressor methyl CpG-binding protein 1 potx
... MBD1 gene was induced by interferon,indicating that these genes are co-ordinately regulated. The interaction was confirmed in vitro and in vivo and wasmapped to the ubiquitin-like domain of p59 ... LexAgene and the human p59 OASL. To identify proteinsthat interact with specific domains of p59 OASL, deletionmutants containing the P-loop, ATP-binding, and UbLdomains, individually or in combination, ... fetal bovine serum (FBS) and 1% penicillin/streptomycin. HeLa and T98G cell lines were grownaccording to ATCC guidelines in DMEM supplementedwith 10% FBS and 1% penicillin/streptomycin.RT-PCR...
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Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... the cells was determined. The cells included the K562 cell line and its Pgp over-expressing subline (K562 ⁄ ADR), and the GLC4 cell line and its MRP1over-expressing subline (GLC4 ⁄ ADR).Innate ... over-expressing subline(K562 ⁄ ADR), the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ADR), the 2008 cell line and its sublines over-expressingMRP1, MRP2 or MRP3, and the cell line HEK293 ... was added and the cells were incubated further for 30 min. The amount of TMRMassociated with the cells was determined as described in Fig. 1. The cells included the K562 cell line and its Pgp...
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Báo cáo khoa học: Interactions between coenzyme B12 analogs and adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum pot
... cofactor, and the Km and kcat for AdoCbi-GDPwere determined in the presence of 20, 70, 100, 150 and 200 lm cofactor. The reaction was initiated by addingl-glutamate and incubating at room ... containing 50% CH3CN. The flowrate was 1 mLÆmin)1. The following profile was used for separation: 2 min isocratic development with 98% A; 5 minlinear gradient from 98% A to 75% A; 15 min linear ... were pro-cessed and analyzed by using topspin 2.1 software (BrukerBioSpin GmbH; Rheinstetten, Germany).Measurement of the binding of coenzymeanalogs to proteinsThe binding of coenzyme analogs...
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Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf
... described in the text. After removal of unbound cobalamin at 0 min (thick solidline), 30 min (thin solid line), 60 min (thin long-dashed line),120 min (thin short-dashed line), 240 min (thin dotted ... damagedcofactors) for free adeninylpentylcobalamin (AdePeCbl)(an inactive coenzyme analog containing the adeninering in the upper axial ligand; a model of intact coen-zyme, AdoCbl) in the presence of ATP and ... between its adenine moiety and the enzymes’s adenine-binding pocket stabilizesthe interaction between the enzyme a and b subunits. In contrast, even in the absence of ADP, the reactivaseforms...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
... informationThe following supporting information is available:Fig. S1. LPS induces the expression of ICAM-1mRNA in cultured Schwann cells. This supporting information can be found in theonline version ... lgÆmL)1aprotinin and 1 lgÆmL)1leupeptin], and clarified by centrifuging at12 000 g for 20 min in a microcentrifuge at 4 °C. Theprotein concentration of the resulting supernatant by theBradford assay ... 4347indicating that LPS induces ICAM-1 expression in SCs, suggest that ICAM-1 may play a role in the focalaccumulation and antigen-induced activation of T cells in inflammatory demyelinating diseases...
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