Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

... 2006 The Authors Journal compilation ª 2006 FEBS Structural and functional roles for b-strand 7 in the a- crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana Yu ... (2001) The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing a- crysta...

Ngày tải lên: 07/03/2014, 12:20

... boxed are the transmembrane domain and the serine ⁄ threonine kinase domain. A. Herpin et al. BMP/activin pathway in Crassostrea gigas FEBS Journal 272 (2005) 3424–3440 ª 2005 FEBS 34 27 Daf-1 ... extracellular domain (C2) also contained 10 cysteines and these are also shown in bold and the cysteine knot is boxed. The C1 and C2 domains appeared to be joined by a ‘...

Ngày tải lên: 07/03/2014, 21:20

... k cat values for nucleotide formation with guanine and hypoxanthine bases, respectively, indicating that Asp1 37 functions as a catalytic base [12]. However, a tight binding of N7 of the purine base and Asp1 37 ... interpreted as GMP because the N2 of the base makes a hydrogen bond to a main chain car- bonyl. Binding of a xanthine base from XMP in the sam...

Ngày tải lên: 15/02/2014, 01:20

... exists as a domain- swapped dimer. The residue D230 is involved in several interactions that are probably crucial for domain swap- ping. A divalent metal ion is found at the active site of the enzyme, ... V94 is found in Tm SurE also. Overall structural features of the protein St SurE is an aba sandwich protein and adopts the Rossmann fold as in archaic a...

Ngày tải lên: 18/02/2014, 14:20

... and part of the His-tag was observed in the B chain. A few amino acid residues are found in disallowed regions in the Ramachandran plot; these are A4 , A1 65, A1 67, B3, B4 and F165, all of which are ... main-chain and medium side-chain restraints. Model building was carried out in o [29] and coot [30]. In chain A, residues 1 and 169– 176 are missing; in...

Ngày tải lên: 18/02/2014, 16:20

... growth factor domains whereas PDGF-C and -D have a unique two -domain structure containing the N-ter- minal CUB domain separated from the C-terminal growth factor domain by a hinge region (Fig. 1A) . PDGF-C ... factor domain, acting as coreceptors for VEGF -A and sem- aphorins (reviewed in [40]). Crystallography studies of the MAPS protein containing two CUB dom...

Ngày tải lên: 19/02/2014, 07:20

... involved in the translocation as a putative effector protein rather than being a component of the T4SS apparatus. Finally, interaction studies indicated that CagI might interact with CagZ and CagG, and weak ... other Cag proteins both belonging to the core apparatus, includ- ing CagX, CagY, CagT, CagV and Cagd, as well as other Cag components such as the ATPase CagE, C...

Ngày tải lên: 06/03/2014, 00:21

... the AAA family of ATPases, a large superfamily of pro- teins involved in the ATP-dependent rearrangement of protein complexes [15– 17] . AAA-proteins are found in all organisms and are essential for ... Pex6pA1(K48 9A) and Pex6pA2(K 778 A) . These invariant lysine residues have been shown to be essential for the biological activity of a number of ATP- and...

Ngày tải lên: 07/03/2014, 16:20

... reveals functional simi- larities and differences between Iba1 and Iba2. We investigated Ca 2+ binding and homodimerization of Iba1 and Iba2. Furthermore, F-actin binding and cross-linking assays ... truncation mutant of neurabin-2 (E). Fig. 6. Calcium-binding assay for Iba1 and Iba2. Iba1, Iba2 and calmodulin where dialyzed against 100 l M CaCl 2 and a trace amount...

Ngày tải lên: 16/03/2014, 06:20

... quantities of protein util- izing the principle of protein dye binding. Anal Biochem 72 , 248–254. 30 Laemmli UK (1 970 ) Cleavage of structural proteins during the assembly of the head of bacteriophage ... phosphoenolpyruvate, 0.2 mm NADH, different concentrations of ATP and NMPs, and 2 units each of pyruvate kinase, lactate dehydrogenase and NDP kinase (forwa...

Ngày tải lên: 16/03/2014, 10:20