Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... morerapid handling using the devices and loss of peptide on the column resin. The only detriment of the peptidesproduced here is the fact that they contain an exo-genous N-terminal methionine. However, ... produc-ing and cloning genes for familial mutants in the 19–23 region of Ab(L1–40) and Ab(L1–42), and expressing and purifying the peptides. The procedure is of course not limited to the peptide ... : 2000 in blocking solution for 30 min. Cells wererinsed in NaCl ⁄ Piseveral times and incubated in blockingserum containing anti-mouse IgG (Vectastain) for a fur-ther 30 min. Staining was...

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... expressing both isoforms of KLK11, and used human a neuroblastoma cell line,KP-N-YN, because of the transfection efﬁciency and expression of both KLK11 isoform 1 and isoform 2 in this cell line. ... from +1379 to +1454. The noncoding exons of exons 1a and 1c contributed to the 5¢ nontranslated region of isoform 1 mRNA in which the common exon 2 contains an initiation codon.Intronic 5¢ consensus ... the regulation and characteristics of these isoforms are unknown. We identiﬁed the ﬁrst three exons (1a, 1b, and 1c) by determining their transcription initiation sites. Exon 1b con-tained the...

Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis docx

... annealed and inserted into the BglII and HindIIIsites of pEGFP-NLS. The oligonucleotide fragment forNES-NLS was ﬂanked with BglII and BamHI, and theninserted into the BamHI site of pGEX-hGFP. ... Microinjection of NLS-containing substrates in a myoblast. The movie represents the NLS microin-jection experiment shown in Fig. 6A.Video S2. Microinjection of NLS-containing substrates in a myotube. The ... activation of CRM1 in myotubes. It is therefore likely that anincrease in Nup358 during myogenesis supports the CRM1 activity and is involved in the activation of nuclear protein export in myotubes.It...

Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot

... processing of the APP family of proteins. BACE1 expression regulated the levels and species of full-lengthAPLP1, APP and APLP2, of their shed ectodomains, and of their mem-brane-bound C-terminal ... cleavage and binding to FE65 regulate the nuclear translocation of the intracellular C-terminal domain (ICD) of the APP family of proteins. Biochem-istry 42, 6664–6673.44 Cao X & Sudhof TC ... loss of BACE1activity. These results indicate that BACE1 is involved in regulating ecto-domain shedding, maturation and trafﬁcking of the APP family of pro-teins. Consequently, whereas inhibition...

Báo cáo khoa học: Puriﬁcation, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... staining on the gel. In the case of activity staining, the gel contained 0.1% (w/v)CMC and the conditions of sample treatment for SDS/PAGE were changed from boiling for 5 min to incubating ... The activity staining indicated that there were no other CMCaseactivities except the GHF 5 cellulase in the larval gut of P. hilaris. The sensitivity of the activity staining used is high, and ... and concentratedfrom the unstained gel, and the protein solution wasincubated with sample buffer for SDS/PAGE, addingreducing agent at 100 °C for 5 min. Then, the proteinsolution was again...

Báo cáo khóa học: Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II potx

... half of the CTDsupports RNA synthesis and capping of the primarytranscript [6–8], whereas the C-terminal half supportssplicing and 3¢ processing of the transcripts [6]. The importance of the C-terminal ... of CDK7/CycH/MAT1 and may prevent the spreading of phosphorylation by this kinase into the C-terminal portion. Thus, the CTD seems to be separatedinto two subdomains. The C-terminal subdomain ... protein kinases are involved directly in the phosphorylation of the CTD and in the regulation of different stages of mRNA synthesis [1,2]. Cyclin dependentkinase (CDK)7, in conjunction with cyclin...

Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc

... (Fig. 5B). Pre-incubation of the nuclear extractwith an anti-Sp1 IgG decreased the intensity of the upper band for both GC-boxes 1 and 2 in both celllines, suggesting that the binding of Sp1, detected ... decreasedby the deletion of GC-box1. The activity was furtherdecreased by the deletion of GC-box2 (Fig. 3B). Asshown in Fig. 3C, the transcriptional activity of the 1 kb of the 5¢-ﬂanking region ... an investigation of the molecular basis of the constitutive and ubiquitous expression of NOX4 in human tissues. We report here the predominant role of the transcription factor speciﬁcity protein...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... mechanism of rhodamine dyes,agents allowing the enhanced uptake of rhodaminedyes inhibited the efﬂux of these dyes from the cells. The efﬂux of rhodamine 123 and TMRM was inhib-ited by NBD-Cl and ... allows the estimation of the effect of intracellular binding on the MDR phenomenon. The data detailed obtained in the present study indicatethat innate cellular MDR interferes successfully with the ... accumulation of dyes, and probably certain drugs, in the cytoplasm. On the other hand, high-afﬁnitybinding of these agents to intracellular receptors over-whelms the low levels of ABC transporters...

Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx

... [16,19,20,23]. The energy of folding these vari-ous forms and the energetic cost of converting oneform to another is of fundamental importance forunderstanding telomere biology and the interactions of small ... Coupling of localfolding to site-speciﬁc binding of proteins to DNA.Science 263, 777–784.50 Lane AN & Jenkins TC (2000) Thermodynamics of nucleic acids and their interactions with ligands. ... istightly linked to their thermodynamic stability.Methods for studying quadruplexfolding and unfolding The folding and unfolding of G-quadruplexes can beconveniently monitored using spectroscopic...

Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-speciﬁc intronic inverted repeats pptx

... distribution of LIRs in both intronic and intergenic regions (see Fig. S1). We also considered the effect of the length of these regions on the distribution. The intronic and intergenic regions were then ... Aluwas included in one arm, and the complete structure of Alu could be retained therein. The proportion of inverted Alus within the LIRs is 6% for intronicregions and 3% for intergenic regions. The ... development and functions of the nervous system are not enriched in chimpanzee and mouse ortho-logs. The ﬁndings of the present study provide insight into the role of intronic LIRs in gene regulation and...

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