Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx
Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... more rapid handling using the devices and loss of peptide on the column resin. The only detriment of the peptides produced here is the fact that they contain an exo- genous N-terminal methionine. However, ... produc- ing and cloning genes for familial mutants in the 19–23 region of Ab(L1–40) and Ab(L1–42), and expressing and purifying the peptides. The proc...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... expressing both isoforms of KLK11, and used human a neuroblastoma cell line, KP-N-YN, because of the transfection efficiency and expression of both KLK11 isoform 1 and isoform 2 in this cell line. ... from +1379 to +1454. The noncoding exons of exons 1a and 1c contributed to the 5¢ nontranslated region of isoform 1 mRNA in which the common exon 2 contains an ini...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis docx
... annealed and inserted into the BglII and HindIII sites of pEGFP-NLS. The oligonucleotide fragment for NES-NLS was flanked with BglII and BamHI, and then inserted into the BamHI site of pGEX-hGFP. ... Microinjection of NLS-containing substrates in a myoblast. The movie represents the NLS microin- jection experiment shown in Fig. 6A. Video S2. Microinjection of NLS-...
Ngày tải lên: 06/03/2014, 01:20
Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot
... processing of the APP family of proteins. BACE1 expression regulated the levels and species of full-length APLP1, APP and APLP2, of their shed ectodomains, and of their mem- brane-bound C-terminal ... cleavage and binding to FE65 regulate the nuclear translocation of the intracellular C-terminal domain (ICD) of the APP family of proteins. Biochem- istry 4...
Ngày tải lên: 15/03/2014, 10:20
Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
... staining on the gel. In the case of activity staining, the gel contained 0.1% (w/v) CMC and the conditions of sample treatment for SDS/ PAGE were changed from boiling for 5 min to incubating ... The activity staining indicated that there were no other CMCase activities except the GHF 5 cellulase in the larval gut of P. hilaris. The sensitivity of the activity s...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khóa học: Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II potx
... half of the CTD supports RNA synthesis and capping of the primary transcript [6–8], whereas the C-terminal half supports splicing and 3¢ processing of the transcripts [6]. The importance of the C-terminal ... of CDK7/CycH/MAT1 and may prevent the spreading of phosphorylation by this kinase into the C-terminal portion. Thus, the CTD seems to be separat...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc
... (Fig. 5B). Pre-incubation of the nuclear extract with an anti-Sp1 IgG decreased the intensity of the upper band for both GC-boxes 1 and 2 in both cell lines, suggesting that the binding of Sp1, detected ... decreased by the deletion of GC-box1. The activity was further decreased by the deletion of GC-box2 (Fig. 3B). As shown in Fig. 3C, the transcriptional acti...
Ngày tải lên: 29/03/2014, 00:20
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... mechanism of rhodamine dyes, agents allowing the enhanced uptake of rhodamine dyes inhibited the efflux of these dyes from the cells. The efflux of rhodamine 123 and TMRM was inhib- ited by NBD-Cl and ... allows the estimation of the effect of intracellular binding on the MDR phenomenon. The data detailed obtained in the present study indicate that innate cel...
Ngày tải lên: 18/02/2014, 13:20
Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx
... [16,19,20,23]. The energy of folding these vari- ous forms and the energetic cost of converting one form to another is of fundamental importance for understanding telomere biology and the interactions of small ... Coupling of local folding to site-specific binding of proteins to DNA. Science 263, 777–784. 50 Lane AN & Jenkins TC (2000) Thermodynamics of nucleic acid...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-specific intronic inverted repeats pptx
... distribution of LIRs in both intronic and intergenic regions (see Fig. S1). We also considered the effect of the length of these regions on the distribution. The intronic and intergenic regions were then ... Alu was included in one arm, and the complete structure of Alu could be retained therein. The proportion of inverted Alus within the LIRs is 6% for intro...
Ngày tải lên: 07/03/2014, 00:20