Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc

... work was supported by a grant from Ministry of Science and Technology of the Spanish Government (SAF 2002–02785), INTAS grant (97 150 4), and the Netherlands’ Organization for Scientific Research. ... negative p aths in the c ycle of subgraphs here reflect the competition of two r eactions for a single species. For example, the competition of protein X and the enzyme E...

Ngày tải lên: 19/02/2014, 16:20

... to CD40 and enhances adenoviral infection of mouse and human DC and B lineage cells. Materials and methods Cell lines Epstein–Barr virus- (EBV-) transformed human B cells were provided by X. Jin ... phase-contrast microscopy were harvested and subjected to three rounds of plaque purification. Virus stocks were expanded and purified by cesium chloride gradient, followed by...

Ngày tải lên: 20/02/2014, 11:20

... MINIREVIEW 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein ligand complexes Kiyoshi Ozawa, Peter S. C. Wu, Nicholas E. Dixon and ... preparation of five samples leads to the assignment of every [ 15 N]-HSQC cross-peak to one of K. Ozawa et al. 15 N-labelled proteins by c...

Ngày tải lên: 07/03/2014, 12:20

... these studies, the direct bind- ing of and the specific requirement for both PPARc and RXRa for efficient complex formation with the PPRE centred at )159 and )148 within Prm3 was veri- fied by examining ... the PPARc(b) and RXR IV half sites centred at )159 and )148, respectively, are required for binding and for 15d-PGJ 2 -inhibition of Prm3 activity and TPb express...

Ngày tải lên: 07/03/2014, 21:20

... L (2004) Regulation of RNA function by aminoacylation and editing? Trends Genet 12, 604–610. 3 Pauling L (1958) The probability of errors in the pro- cess of synthesis of protein molecules. In ... amino acid and tRNA substrates [1]. The mechanism(s) by which the family of aminoacyl-tRNA synthetases maintains the accuracy of protein biosynthesis has been the subject...

Ngày tải lên: 18/02/2014, 13:20

... pre- and suffixes. A comparison of different levels of supervision for morphology learning on Zulu has been carried out by Spiegler et al. (2008). Our two algorithms, PROMODES and PROMODES-H, perform ... underlying sequence of tosses which determine the morpheme boundaries. We are applying the notion of a prob- 1 PROMODES stands for PRObabilistic MOdel for different DEgrees...

Ngày tải lên: 20/02/2014, 04:20

... MTGs generate tuples of parse trees that are isomorphic up to reordering of sibling nodes and deletion. Figure 2 shows two representations of a tree that might be generated by an MTG in GCNF for the imperative sentence ... dimen- sions of a vector. E.g., is a vector spanning dimensions 1 through . See Melamed (2003) for definitions of cardinality, d-span, and the operators...

Ngày tải lên: 20/02/2014, 16:20

... analyzed and given deep semantic relations. We use 63 deep semantic cases[Inoue]. Correspondences of Japanese and English are made by several linguistic units, for example words, sentences and ... set of clusters which are automatically obtained. B : a set ofclusters which are used in our interpreting telephony system. p • the number of clusters of a set A q : the n...

Ngày tải lên: 20/02/2014, 21:20

Ngày tải lên: 22/02/2014, 03:20

... in the synthesis of NAD + from nicotin- amide. Visfatin ⁄ PBEF ⁄ NAMPT is synthesized and secreted in adipose tissue by adipocytes, and mostly by macrophages, and circulates in the plasma of humans ... regulation of NAD + synthesis is critical for several aspects of cell physiology [15] . Macrophages, crucial cells in the development of inflammatory and metabolic di...

Ngày tải lên: 06/03/2014, 22:21