Báo cáo khoa học: The CssRS two-component regulatory system controls a general secretion stress response in Bacillus subtilis pdf

... diluted in fresh medium and samples were taken at different intervals for absorbance readings at 600 nm and b-galactosidase activity determinations. To assay b-galactosidase activity, a semiautomated ... derivative containing the amyQ gene of B. amyloliquefaciens, encoding AmyQ with an artificial alanine-rich signal peptide; Km r [26] pP43LatIL3 pMA5 derivative, containing the hIL-3 ge...

Ngày tải lên: 07/03/2014, 12:20

... GCTTTGTTCGTGCAAATTTCC 55 35 CTGCAAATATCTCTGGGAAGA TRPC5 CAGCATTGCGTTCTGTGAAAC 55 35 CAGAGCTATCGATGAGCCTAAC TRPC6 GACATCTTCAAGTTCATGGTCATA 55 35 ATCAGCGTCATCCTCAATTTC TRPC7 CAGAAGATCGAGGACATCAGC 55 ... 25 TGCACTTGGCGTTCTTGT TRPC1 GATTTTGGAAAATTTCTTGGGATGT 55 35 TTTGTCTTCATGATTTGCTATCA TRPC2 CATCATCAT-GGTCATTGTGCTGC 55 35 GGTCTTGGTCAGCTCTGTGAGTC TRPC3 GACATATTCAAGTTCATGGTCCTC 55 35 ACATCACTGTCA...

Ngày tải lên: 07/03/2014, 05:20

... chaperones that facilitate protein fold- ing and suppress protein aggregation, this response plays a major role in maintaining protein homeostasis. The heat shock response is regulated mainly at the level ... HSF-mediated mechanisms of cellular adaptation to stress in verte- brates by comparing mammalian and avian cells, and also review HSF-mediated mechanisms of adaptation to...

Ngày tải lên: 18/02/2014, 04:20

... integrin alpha10-for, 5¢-CATGAGGTT CACCGCATCACT-3¢, and integrin alpha10-rev, 5¢-AAGG CAAAGGTCACAGTCAAGG-3¢ (annealing temperature 64 °C). The expression ratios of the analyzed genes were calculated ... 5¢-GAT CCTCGCAGGGACTACA-3¢, and AP- 2a- rev, 5¢-GTTGG ACTTGGACAGGGAC-3¢ (annealing temperature 60 °C); Sox9-for, 5¢-CGAACGCACATCAAGACGA-3¢, and Sox9- rev, 5 ¢-AGGTGAAGGTGGAGTAGAGGC-3¢ (ann...

Ngày tải lên: 18/02/2014, 08:20

... A part- nership with a catalytic domain is observed in the RNA-dependent protein kinase (PKR) that contains a serine ⁄ threonine kinase domain [7–9] and in the dsRNA-specific adenosine deaminases ... ⁄ Drosha [12–14] RNase III domain a dsRNA processing in RNAi ⁄ miRNA pathway ADAR1 & 2 [10] A fi I deaminase domain RNA editing RHA [11] DEXH helicase domain Transcriptional...

Ngày tải lên: 19/02/2014, 17:20

... ATCCTTTATGGTGAGATCAGCTTCAATATCCGTCCA Vps4–TRP F TTAAAGGCTATCAAATCGCAAGAACAGTTCACTAGA Vps4–TRP R TCTAGTGAACTGTTCTTGCGATTTGATAGCCTTTAA Vps4–RDF F GAAGCAAGAACAGTTCACTTAGTCAATTGATTAACGTG Vps4–RDF R CACGTTAATCAATTGACTAAGTGAACTGTTCTTGCTTC Table ... interacting and trafficking domain required for endosome recruitment, an AAA domain containing the ATPase catalytic site and a b domain, and a C-te...

Ngày tải lên: 07/03/2014, 05:20

... h, and D 595 readings taken every 30 min to assess the rate of bacterial growth. The D values were calculated relative to the initial readings in the same samples. An asterisk denotes mean values ... of Achatinin, a 9-O-acetyl sialic acid-binding lectin, with lipopolysaccharide in the innate immunity of Achatina fulica snails. Mol Immunol 37, 745–754. P. Melamed et al. C-type...

Ngày tải lên: 07/03/2014, 17:20

... Stereodiagrams of the interactions of AMP in the RNase A active site. The side chains of protein residues involved in ligand binding are shown as ball-and-stick models. Bound waters are shown as black ... Thus, although the Table 4. Van der Waals interactions of IMP and AMP in the active site of RNase A. IMP ⁄ AMP atom RNase A IMP RNase A AMP IMP Mol I IMP Mol II IMP Mol...

Ngày tải lên: 07/03/2014, 21:20

Ngày tải lên: 09/03/2014, 01:20

... mLÆmin )1 with a linear gradient from 100% B to 43% B within 45 min, to 0% B within 1 min, then increasing the flow rate to 2 mLÆmin )1 within 1 min and maintaining these final conditions for another ... source of apocarotenoids. In mam- mals, the synthesis of apocarotenoids, including retinoic acid, is initiated by the b-carotene cleavage oxygenases I and II catalyzing either a cen...

Ngày tải lên: 15/03/2014, 23:20