Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt

... localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 Judit Rumszauer, ... [1,2]. Thermus represent- atives, such as Thermus thermophilus strain HB27, Thermus thermophilus HB8, Thermus flavus AT62, Thermus caldoph...

Ngày tải lên: 07/03/2014, 12:20

... CA protein sequences. Iden- tical and similar amino acids shared by at least 50% of the isoforms are shown in black and grey, respectively. Histidine residues involved in zinc binding in the catalytic ... 94, 96 and 119 in CAII from Homo sapiens) which are directly involved in binding the zinc cofac- tor, are conserved in the two R. pachyptila sequences (positi...

Ngày tải lên: 18/02/2014, 16:20

... Htelo (Fig. S6). These findings indicate the importance of the guanidinium group of the Arg in RGG3 for binding to the G-quadruplex. In conclusion, EWS appears to be a DNA- and RNA-binding protein that ... DNA and RNA in vitro. We also determined that the specificity of G-quadruplex recognition depends on the guanidini- um group of the Arg in the RGG domain...

Ngày tải lên: 15/02/2014, 01:20

... domain of t-bet, the STAT protein inter- action domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and ... proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved in the induction of dimeriza...

Ngày tải lên: 16/02/2014, 09:20

... bacteria. The difference between these two mutants in bacterial clearance might reflect differences in the integrity of their outer membrane, binding activity and sensitivity of the murine comple- ment-mediated ... containing the various agents were plated on the lawn in triplicate at 37 °C for 18 h. Sensitivity was assessed by measuring the diameter of the zone of...

Ngày tải lên: 18/02/2014, 14:20

... representing the 5¢- and 3¢-UTRs are shown in white in the genomic gene and the cDNA. Intron size in bp, domain size in bp and domain size in amino acids are indicated at the bottom of each schematic ... two-hybrid and yeast-three hybrid experiments showing SmSmad1B protein interactions. The following cri- teria were utilized in this table for designating the...

Ngày tải lên: 18/02/2014, 16:20

... number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tubulin myosin and profilin) were differentially expressed in C. ... assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these chaperones c...

Ngày tải lên: 18/02/2014, 16:20

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat ... OMM-64 and otolin-1, are expressed in the same cells in the inner ear sacculus and are secreted into the extracellu- lar matrices of the inner ear. In the oto...

Ngày tải lên: 18/02/2014, 17:20

... [4–6]. In order to improve the biological production method, a detailed understanding of the biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions ... reactions and the genes encoding these enzymes. The biosynthesis of triterpene saponins involves the initial cyclization of 2,3-oxidosqualene, a common pre- curso...

Ngày tải lên: 19/02/2014, 07:20

... DNA binding, and compared the cDNAs of IFN-treated cells over- expressing intact STAT2 to those of IFN-treated cells overexpressing mutated STAT2 lacking the DNA binding domain. The IFN-inducible expression ... expression is IFN-inducible and independent of ISGF3 but highly dependent on the STAT2 DNA binding domain. This report is the first analysis of the contribution...

Ngày tải lên: 19/02/2014, 07:20