... 2006 FEBS Biochemical characterization of human3-methylglutaconyl-CoA hydratase and its role in leucinemetabolismMatthias Mack1, Ute Schniegler-Mattox2, Verena Peters3, Georg F. Hoffmann3, ... In addition, a mutant form of AUH (A240V) derived from an MGA1 patient wastested using 3-MG-CoA.ResultsOverexpression of AUH in Escherichia coli andpurification of the corresponding gene productThe ... and it was thus assumed thatthe mature form of human AUH in brain has amolecular weight of 32 kDa (AUHp32) [15]. For thekinetic characterization of AUH described in the workat hand, AUH was...