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Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf

Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf

Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf

... et al. Biochemical characterization of annexin B1 FEBS Journal 273 (2006) 3238–3247 ª 2006 The Authors Journal compilation ª 2006 FEBS 3247 Biochemical characterization of annexin B1 from Cysticercus ... affinity of the interactionwith heparin. Subsequent binding of annexin A5 to themembrane surface releases the glycosaminoglycan from A. Winter et al. Biochemical characterization of annexin B1 FEBS ... of (reversibly) bound protein was determined from the EDTA eluate. As seen from Fig. 4B, the datafor annexin B1 suggests a cooperative binding beha-Fig. 1. Amino acid alignment of annexin B1 from...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... 2006 FEBS Biochemical characterization of human3-methylglutaconyl-CoA hydratase and its role in leucinemetabolismMatthias Mack1, Ute Schniegler-Mattox2, Verena Peters3, Georg F. Hoffmann3, ... In addition, a mutant form of AUH (A240V) derived from an MGA1 patient wastested using 3-MG-CoA.ResultsOverexpression of AUH in Escherichia coli andpurification of the corresponding gene productThe ... and it was thus assumed thatthe mature form of human AUH in brain has amolecular weight of 32 kDa (AUHp32) [15]. For thekinetic characterization of AUH described in the workat hand, AUH was...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... of the enzyme for that cofactor and theapparent absence of a redox process as part of the catalyticcycle. The substrate binding site of the type II enzymeremains veiled. However, a patch of ... assay of type II IPP isomerase from B. subtilis.A, part of the1H NMR spectrum of the reaction mixture (lower lane)obtained from IPP (1H NMR signals, see upper lane) by the catalyticaction of ... were purchased from Biomol (Hamburg, Germany). FMN was obtained from Sigma (Steinheim, Germany).Cloning and expression of theidi-2gene from B. subtilisA DNA segment extending from bp position...
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx

Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx

... specificity and enzymatic properties of USP7As part of the characterization of USP7 biochemical properties, we have measured its kinetic parameters forthe hydrolysis of ubiquitin C-terminal 7-amido-4-meth-ylcoumarin ... (208–1102) of purified recombinant proteinper litre of insect cell culture. In addition, an average of 7 mg USP7 208-560 per litre of Escherichia coli fer-mentation broth was obtained from the ... was performed by searching the MS data againsta curated version of the International Protein Index Biochemical characterization of USP7 A. Ferna´ndez-Montalva´n et al.4266 FEBS Journal 274...
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Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... assemblyensures the transfer of electrons from different sourcesto oxygen by the respiratory chain complexes and thecoupling of proton uptake from the matrix compart-ment [28].The nature of the quinone ... of recombinant DHODH should expedite discov-ery of more potent agents for growth controlstrategies in C. albicans, and permit the screening of a large number of compounds, the examination of structure–activity ... (clas-sified as family 1 DHODH), the activity of which isindependent of ubiquinone and the presence of oxygen[15–17]. This feature promotes growth of this yeastunder anaerobic conditions. Saccharomyces...
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Báo cáo khoa học: Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes potx

Báo cáo khoa học: Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes potx

... Authors Journal compilation ª 2007 FEBS 1201 Biochemical characterization of rice trehalose-6-phosphatephosphatases supports distinctive functions of these plantenzymesShuhei Shima1,2, Hirokazu ... function of trehalosebiosynthesis in the regulation of starch biosynthesishas recently been revealed. Trehalose feeding wasfound to induce expression of ApL3, encoding a largesubunit of ADP-glucose ... vitro functionality of its translated prod-uct. We also describe unique kinetic and biophysicalproperties of the plant TPPs.ResultsIsolation of rice OsTPP2Completion of the rice genome sequence...
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Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

... content of approximately5.4% (Table 2). From the overall sugar content(HPAEC) and the estimated sugar contents of the indi-vidual glycoforms (MALDI-TOF-MS), the proportions of glycoforms I ... properties of SPC4.The absorption spectrum of purified SPC4 was recorded in 50 mMsodium acetate pH 5. The inset shows the spectrum of the extract-ed heme. (B) MALDI-TOF-MS analysis of SPC4 heme. Characterization ... PS(1997) Characterization of two major cationic peroxi-dases from cell suspension cultures of Vaccinium myrtil-lus. Plant Sci 122, 1–10.25 Welinder KG (1976) Covalent structure of the glycopro-tein...
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Báo cáo khoa học: Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase ppt

Báo cáo khoa học: Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase ppt

... of protein per lL of sample); lane2: human erythrocyte AChE standard(4.5 lg of protein per l L of sample); lane 3,extract of solubilized membranes of HUVECs (8.5 lg of protein per lane). Characterization ... Biotechnology) of protein extracts of solubilized membranes of HUVECS (30 lg of protein per lane),with dithiothreitol protein reduction.F. A. Carvalho et al. Characterization of HUVECs membrane ... Coomassie blue staining of protein extracts of solubilized membranes of HUVECS (30 lg of protein per lane),Human recombinant AChE standard (4.5 lg of protein per lL of sample) and human erythrocyte...
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Báo cáo khoa học: Biochemical characterization of a U6 small nuclear RNA-specific terminal uridylyltransferase potx

Báo cáo khoa học: Biochemical characterization of a U6 small nuclear RNA-specific terminal uridylyltransferase potx

... incorporation of GMP residues into the opposite3¢-end of the mutant RNA.A second mutation of the U6 substrate RNA consisted of the introduction of two additional AMP nucleotides in front of the oligo(A) ... Puri-fication, characterization and cloning of the cDNA of humanSRP RNA-3¢ adenylating enzyme. J. Biol. Chem. 276, 21791–21796.980 R. Trippe et al.(Eur. J. Biochem. 270) Ó FEBS 2003 Biochemical characterization ... asize range from 70 to 130 kDa for elution from this column.Fig. 3. Substrate analysis of the TUTase with structural mutants of theU6 snRNA. (A) Proposed secondary structure of U6 snRNA [25].Mutant...
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Báo cáo khoa học: Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB pot

Báo cáo khoa học: Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB pot

... in a 15-fold increase in the yield of thesoluble protein. Pantetheinylation of the ACP in ACP-CON didomain was verified using MALDI-TOF of atryptic fragment of the purified protein (data notshown). ... Expression of the LovBKS domain devoid of the MAT domain resulted inthe formation of inclusion bodies, which was recentlynoted for the KS domains of other megasynthases[23].Proteolytic cleavage of ... biosynthesis of novel polyketides. Biochemical studies and engineering of the fungalmegasynthases are more difficult than the bacterialcounterparts for several reasons. First, the intrinsic size of these...
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