Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf

... et al. Biochemical characterization of annexin B1 FEBS Journal 273 (2006) 3238–3247 ª 2006 The Authors Journal compilation ª 2006 FEBS 3247 Biochemical characterization of annexin B1 from Cysticercus ... affinity of the interaction with heparin. Subsequent binding of annexin A5 to the membrane surface releases the glycosaminoglycan from A. Winter et al. Biochem...

Ngày tải lên: 07/03/2014, 12:20

... 2006 FEBS Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism Matthias Mack 1 , Ute Schniegler-Mattox 2 , Verena Peters 3 , Georg F. Hoffmann 3 , ... In addition, a mutant form of AUH (A240V) derived from an MGA1 patient was tested using 3-MG-CoA. Results Overexpression of AUH in Escherichia coli and purification of the corres...

Ngày tải lên: 19/02/2014, 07:20

... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. The substrate binding site of the type II enzyme remains veiled. However, a patch of ... assay of type II IPP isomerase from B. subtilis. A, part of the 1 H NMR spectrum of the reaction mixture (lower lane) obtained from IPP ( 1 H NMR signals, see upper lane) by the...

Ngày tải lên: 19/02/2014, 13:20

... specificity and enzymatic properties of USP7 As part of the characterization of USP7 biochemical properties, we have measured its kinetic parameters for the hydrolysis of ubiquitin C-terminal 7-amido-4-meth- ylcoumarin ... (208–1102) of purified recombinant protein per litre of insect cell culture. In addition, an average of 7 mg USP7 208-560 per litre of Escherichia coli fe...

Ngày tải lên: 07/03/2014, 05:20

... assembly ensures the transfer of electrons from different sources to oxygen by the respiratory chain complexes and the coupling of proton uptake from the matrix compart- ment [28]. The nature of the quinone ... of recombinant DHODH should expedite discov- ery of more potent agents for growth control strategies in C. albicans, and permit the screening of a large number of com...

Ngày tải lên: 07/03/2014, 12:20

... Authors Journal compilation ª 2007 FEBS 1201 Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes Shuhei Shima 1,2 , Hirokazu ... function of trehalose biosynthesis in the regulation of starch biosynthesis has recently been revealed. Trehalose feeding was found to induce expression of ApL3, encoding a large...

Ngày tải lên: 16/03/2014, 11:20

... content of approximately 5.4% (Table 2). From the overall sugar content (HPAEC) and the estimated sugar contents of the indi- vidual glycoforms (MALDI-TOF-MS), the proportions of glycoforms I ... properties of SPC4. The absorption spectrum of purified SPC4 was recorded in 50 m M sodium acetate pH 5. The inset shows the spectrum of the extract- ed heme. (B) MALDI-TOF-MS analysis...

Ngày tải lên: 16/03/2014, 13:20

... of protein per lL of sample); lane 2: human erythrocyte AChE standard (4.5 lg of protein per l L of sample); lane 3, extract of solubilized membranes of HUVECs (8.5 lg of protein per lane). Characterization ... Biotechnology) of protein extracts of solubilized membranes of HUVECS (30 lg of protein per lane), with dithiothreitol protein reduction. F. A. Carvalho et al....

Ngày tải lên: 16/03/2014, 23:20

... incorporation of GMP residues into the opposite 3¢-end of the mutant RNA. A second mutation of the U6 substrate RNA consisted of the introduction of two additional AMP nucleotides in front of the oligo(A) ... Puri- fication, characterization and cloning of the cDNA of human SRP RNA-3¢ adenylating enzyme. J. Biol. Chem. 276, 21791– 21796. 980 R. Trippe et al.(Eur. J. Biochem....

Ngày tải lên: 17/03/2014, 09:20

... in a 15-fold increase in the yield of the soluble protein. Pantetheinylation of the ACP in ACP- CON didomain was verified using MALDI-TOF of a tryptic fragment of the purified protein (data not shown). ... Expression of the LovB KS domain devoid of the MAT domain resulted in the formation of inclusion bodies, which was recently noted for the KS domains of other megasynthases [23...

Ngày tải lên: 30/03/2014, 08:20