Báo cáo khoa học: Puriﬁcation and characterization of the cy

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... properties; thermotolerance and insensitivity to inhibition by downstream products, citrulline and arginine. Results The enzyme involved in catalysis of the ﬁfth step of citrulline biosynthesis in wild ... [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32]. In plants, information on the localization of citrul...

Ngày tải lên : 20/02/2014, 03:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... that the major binding site of the lectin was sialic acid on the surface of erythrocytes. The binding speciﬁcity of crab lectin Inhibition studies with various sugars was helpful in deducing the ... NeuAc. Discussion The presence of naturally occurring agglutinins in the hemolymph of several crustaceans has been well known since the beginning of the 20th cent...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... 1897 Identification of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences of the four polypeptides present in the purified enzyme preparation were determined by Edman ... DsrK, and HmcF clearly identified the t wo typical CXXCXXCXXXCP binding motifs for [4Fe-4S] clusters in the N-terminal part of these proteins. AF502...

Ngày tải lên : 21/02/2014, 03:20

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Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... min )1 Æmg )1 protein. The sub- strate specificity of rec. ZHE1 was similar to that of the protease contained in hatching liquid. This result supported the findings of the purification indicating that ... shifted seven amino acid residues to the C-terminal from the site B. Thus, the cleaving sites obtained from the 90 min incubation and the post-hatching egg envelop...

Ngày tải lên : 07/03/2014, 04:20

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Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... Vasconcellea spp. : (1) a higher protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the protein concentration ... of enzymes in the latex of Vas- concellea fruits, but in addition also results from the presence of other cysteine proteinases or isoforms....

Ngày tải lên : 07/03/2014, 11:20

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Báo cáo khoa học: Puriﬁcation and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx

... properties. Therefore, TcoCB6 and daikon CBCP are distinct from one another in terms of the role of the one histidine in the occluding loop. As shown in Figs 3 and 4, the amino acids around the subsites ... activation of cysteine proteases by removal of the propeptide [35,36]. The autoprocessing activity of human cathepsin B is impaired following deletion...

Ngày tải lên : 16/03/2014, 04:20

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Báo cáo khoa học: Puriﬁcation and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc

... M. (1978) Oxygen and nitrate in utilization by Bacillus licheniformis of the arginase and arginine deiminase routes of arginine catabolism and other factors aﬀecting their syntheses. J. Bacteriol. ... as described in the Materials and methods. Binding of the recombinant protein to the column was apparent from the loss of a protein of  40 kDa in the ﬂow- th...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khoa học: Puriﬁcation and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes doc

... Cu 2+ and Ba 2+ were strong inhibitors of the analysed enzymes, whereas the presence of monovalent ions in the incubation mixture did not affect the rate of ATP and ADP hydrolysis (Table 4). ATP and ... ATP and ADP and the product of ATP and ADP hydrolysis is AMP – the compound with a very low afﬁnity for P2 receptors. The above results conﬁrm the partici...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo khoa học: Puriﬁcation and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major doc

... with the DC-protein assay kit using BSA as standard. The concentration of puriﬁed and synthetic chrysophsin-1 and chrysophsin-2 was obtained from the A 280 [24], and that of chrysophsin-3 with the ... localization of chrysophsins in the gills of the red sea bream. Adjacent sections in the gills were immunostained with chrysophsin-1 antibody (A, C, E and F) a...

Ngày tải lên : 23/03/2014, 20:22

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Báo cáo khoa học: Puriﬁcation and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt

... become increasingly important in understanding the biological roles and structure of the glycoaminoglycans (and proteoglycan), which are involved in the well known anticoagulant activity [7] and the regulation ... human intestine and it contains several kinds of GAG degrading enzymes including heparin, heparan sulfate, acharan sulfate and chondroitin sulfate [13–16]....

Ngày tải lên : 23/03/2014, 21:20

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Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp ppt

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf