Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp ppt

... properties; thermotolerance and insensitivity to inhibition by downstream products, cit- rulline and arginine. Results The enzyme involved in catalysis of the fifth step of citrulline biosynthesis in wild ... [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32]. In plants, information on the localization of citrul...

Ngày tải lên: 20/02/2014, 03:20

... that the major binding site of the lectin was sialic acid on the surface of erythrocytes. The binding specificity of crab lectin Inhibition studies with various sugars was helpful in deducing the ... NeuAc. Discussion The presence of naturally occurring agglutinins in the hemolymph of several crustaceans has been well known since the beginning of the 20th cent...

Ngày tải lên: 21/02/2014, 00:20

... 1897 Identification of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences of the four polypeptides present in the purified enzyme preparation were determined by Edman ... DsrK, and HmcF clearly identified the t wo typical CXXCXXCXXXCP binding motifs for [4Fe-4S] clusters in the N-terminal part of these proteins. AF502...

Ngày tải lên: 21/02/2014, 03:20

... min )1 Æmg )1 protein. The sub- strate specificity of rec. ZHE1 was similar to that of the protease contained in hatching liquid. This result supported the findings of the purification indicating that ... shifted seven amino acid residues to the C-terminal from the site B. Thus, the cleaving sites obtained from the 90 min incubation and the post-hatching egg envelop...

Ngày tải lên: 07/03/2014, 04:20

... Vasconcellea spp. : (1) a higher protein content in their latex; and (2) the pres- ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the protein concentration ... of enzymes in the latex of Vas- concellea fruits, but in addition also results from the presence of other cysteine proteinases or isoforms....

Ngày tải lên: 07/03/2014, 11:20

... properties. Therefore, TcoCB6 and daikon CBCP are distinct from one another in terms of the role of the one histidine in the occluding loop. As shown in Figs 3 and 4, the amino acids around the subsites ... activation of cysteine proteases by removal of the propeptide [35,36]. The autoprocessing activity of human cathepsin B is impaired following deletion...

Ngày tải lên: 16/03/2014, 04:20

... M. (1978) Oxygen and nitrate in utilization by Bacillus licheniformis of the arginase and arginine deiminase routes of arginine catabolism and other factors affecting their syntheses. J. Bacteriol. ... as described in the Materials and methods. Binding of the recombinant protein to the column was apparent from the loss of a protein of  40 kDa in the flow- th...

Ngày tải lên: 16/03/2014, 16:20

... Cu 2+ and Ba 2+ were strong inhibitors of the analysed enzymes, whereas the presence of monovalent ions in the incubation mixture did not affect the rate of ATP and ADP hydrolysis (Table 4). ATP and ... ATP and ADP and the product of ATP and ADP hydrolysis is AMP – the compound with a very low affinity for P2 receptors. The above results confirm the partici...

Ngày tải lên: 17/03/2014, 10:20

... with the DC-protein assay kit using BSA as standard. The concentration of purified and synthetic chrysophsin-1 and chrysophsin-2 was obtained from the A 280 [24], and that of chrysophsin-3 with the ... localization of chrysophsins in the gills of the red sea bream. Adjacent sections in the gills were immunostained with chrysophsin-1 antibody (A, C, E and F) a...

Ngày tải lên: 23/03/2014, 20:22

... become increasingly important in understanding the biological roles and structure of the glycoaminoglycans (and proteoglycan), which are involved in the well known anticoagulant activity [7] and the regulation ... human intestine and it contains several kinds of GAG degrading enzymes including heparin, heparan sulfate, acharan sulfate and chondroitin sulfate [13–16]....

Ngày tải lên: 23/03/2014, 21:20