Báo cáo khoa học: Design of expression vectors for RNA interference based on miRNAs and RNA splicing potx

... Design of expression vectors for RNA interference based on miRNAs and RNA splicing Guangwei Du, Joshua Yonekubo, Yue Zeng, Mary Osisami and Michael A. Frohman Department of Pharmacology and ... precursors of microRNAs (miRNAs) driven by polymerase II promoters. We have designed new RNAi vectors, designated pSM155 and pSM30, that take into considera- tion...

Ngày tải lên: 07/03/2014, 11:20

... presents an alternative to decod- ing that is based on transformation rule application in section 2. In section 3 we propose a paraphrase generation method for this paradigm based on an algorithm ... factor and for which there is no strong evaluation function. For the same reasons, this algorithm sounds in- teresting for paraphrase generation. In particular, it does not put...

Ngày tải lên: 17/03/2014, 02:20

... televi- sion, and that 89% of those requests included words belonging to specific categories such as program title, genre, performer, station, time, and TV operation commands. The remaining 17% of ... super-unidirectional micro- phone and a speaker, and communicates and acti- vates the speech recognition and voice synthesis, and dialogue processing of the system. The IF...

Ngày tải lên: 31/03/2014, 03:20

... productions, phrase selection productions, syntax selection productions, ana- phora selection productions, verb morphology produc- tions, punctuation selection productions, writing produc- tions, ... be thought of as n- dimensional propositions, and 2) dynamic knowledge structures, or production rules, which perform pattern- recognition operations on n-dimensional propositions,...

Ngày tải lên: 21/02/2014, 20:20

... normalized form for NPs and PPs. 4.4 CONSIDERATIONS FOR TRANSLATION The goal of the system, and perhaps of MT in general, has to be to carry over the information content from SL to TL, to ... translation. 3. BRIEF DESCRIPTION OF THE SYb-i~4 Modem transfer -based MT systems are based on the following design principles : (i) modularity, e.g. separation of linguis...

Ngày tải lên: 17/03/2014, 19:21

... extent of signaling required for such long-distance coordination is many orders of magnitude greater than the size of the participating cells; for example, coordinated con- tractions of the intestine ... studies of the following: (a) slow-wave depolariza- tions that underlie rhythmic contractions of gastric smooth muscle; and (b) membrane depolarizations that drive rhythmic...

Ngày tải lên: 16/02/2014, 09:20

... the backbone of the bound peptide is forced into a turn conformation by a Pro at P 3 which favors binding to the active site. Furthermore, this turn conformation allows the interaction of bulky ... at 30 °C and the flow rate was 300 nLÆ min )1 . Detection was performed by UV absorption at 214 nm and 280 nm. Between 8 min and 75 min of the run, 191 fractions of the effluent (each...

Ngày tải lên: 16/02/2014, 14:20

... demonstrate strong modulation of expression after stimulation with LPS and LP. LP and LPS mediate immunomodulating functions, at least in part, through regulation of RGS1 and RGS2 expression. Results RGS1 ... after stimulation with LP and LPS. Expression patterns of RGS1 and RGS2 after activation of TLR3 and TLR9 To investigate the expression levels of RGS1 and...

Ngày tải lên: 18/02/2014, 13:20

... [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for study- ing interactions between small molecules and proteins. The latter is a particular application of ‘post-transla- tional ... leading to the formation of methione-sulfoxide. During a second, identical separa- tion (the secondary COFRADIC separation), such oxidized methionyl peptides undergo a hydrophilic...

Ngày tải lên: 18/02/2014, 16:20

... this, UV-irradiation of the cells (2.4 JÆm )2 ), although inducing a strong activation of JNK1/2 and only a very weak phosphorylation of p38, had no effects on C TGF expression, which ruled out ... CTGF/CCN2 expression occurs via increased transcription and/ or by stabilization of the CTGF transcripts and t he role of RhoA GTPase and p38 signaling in such a regulation, n...

Ngày tải lên: 19/02/2014, 16:20