Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... 1084–1092. 35 Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N, Miyashita K & Watanabe T (2006) Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S. coelicolor ... including chitinases from Burkholderia gladioli, Vibrio cholerae, Haemophi- lus influenzae, and Pseudomonas aeruginosa. Although many plant family 19 chitinas...

Ngày tải lên: 07/03/2014, 11:20

... 175–186. 2 Banerjee R, Frasca V, Ballou DP & Matthews RG (199 0) Participation of cob(I)alamin in the reaction cat- alyzed by methionine synthase from Escherichia coli :a steady-state and rapid reaction ... (Wyatt Technologies, Santa Barbara, CA, USA). AUC using an Optima XLA ultracentrifuge was used to assess the overall shape of the molecules and to determine if there was an...

Ngày tải lên: 23/03/2014, 09:21

... dissociation and denaturation of Hc. The sigmoidal plot of A 340 ⁄ A 280 vs. salt concentration can be interpreted in terms of a conformational transition between a native oligomeric state and an ... from crustaceans living under normoxic conditions: values of )0.70, )0.83, )0.57, and )0.68 have been measured for Can. pagurus, Car. maenas [50,51] Par. camtschaticae [1...

Ngày tải lên: 23/03/2014, 13:20

... 275–283. 10 Kuwada M, Teramoto T, Kumagaye KY, Nakajima K, Watanabe T, Kawai T, Kawakami Y, Niidome T, Sawada K, Nishizawa Y et al. (199 4) Omega-agatoxin- TK containing D-serine at position 46, but ... crosspeaks were assigned and inte- grated, with concomitant cycles of structure calcula- tions for evaluation of distance and angle constraint violations as well as assignments of...

Ngày tải lên: 18/02/2014, 17:20

... Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at University of California Los Angeles, USA Keywords: antigenic properties; bacterial expression; MALDI-TOF ... half-life of IgG and albumin [1–3], is a major histocompatibility complex (MHC) class I-related receptor consisting of a heavy chain (HC) with three ectodomains (a1 , a2...

Ngày tải lên: 16/03/2014, 06:20

... P. & Sampieri, F. (199 1) An anti-insect toxin purified from the scorpion Androctonus australis Hector also acts on the alpha- and beta-sites of the mammalian sodium channel: sequence and circular ... following: forward primer A1 , 5¢-GCC GGATCCTGATTGCCTA GAAGATGA-3¢; reverse primer A2 , 5¢-GCC CTCGAG TCAACCGCATGTATTACTTTCAG-3¢.Theforward and reverse primers were preceded by B...

Ngày tải lên: 31/03/2014, 09:20

... Hedderich, R. & Metcalf, W.W. (2002) Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation. ... the hydrogenase subunits. A densitometric analysis revealed a molar ratio of CooS to CooH, the catalytic subunit of the hydrogenase, of 2.1 ± 0.1 : 1 and a molar ratio...

Ngày tải lên: 23/03/2014, 21:20

... each possible pair of structures, and was divided into several conformational families. The average structure was taken from each family and chosen as starting structures for the calculation of ... Chow 2 , Teruna J. Siahaan 3 and Seetharama D. S. Jois 1 1 Department of Pharmacy and 2 Department of Microbiology, National University of Singapore, Singapore; 3 Department...

Ngày tải lên: 19/02/2014, 13:20

... as a substrate, and the absorbance was measured at 490 nm by using a Spectramax plate reader and software (Molecular Devices, Sunnyvale, CA, USA). All values were interpolated from either a log- log ... the average n molÆmg )1 with standard deviations (±) obtained from quantifying and averaging areas under specific peaks from GLC analysis of four separate samples of LGL B...

Ngày tải lên: 19/02/2014, 16:20

... C, Araki M, Sugasawa K, van der Spek PJ, Yamada A, Uchida A, Maekawa T, Bootsma D, Hoeij- makers JH & Hanaoka F (199 7) Identification and char- Dynamic structure of XPC-binding domain of hHR23B ... higher than that of XPCB–hHR2 3A (Fig. 5). The total surface area of all these structures is very similar; XPCB–hHR23B,  41 nm 2 , XPCB–hHR23As from Waters et al. and Kamion...

Ngày tải lên: 07/03/2014, 17:20