Báo cáo khoa học: The Rieske protein from Paracoccus denitrificans is inserted into the cytoplasmic membrane by the twin-arginine translocase doc

... (2006) 4817–4830 ª 2006 The Authors Journal compilation ª 2006 FEBS The Rieske protein from Paracoccus denitrificans is inserted into the cytoplasmic membrane by the twin-arginine translocase Julie ... chains. Based on the presence of two consecutive arginines, it was argued that the ISP of Paracoccus denitrificans, a Gram-negative soil bacterium, is...

Ngày tải lên: 07/03/2014, 11:20

... gives rise to stable dimers which in turn dissociate into stable monomers when the pH is lowered further. Quite unexpectedly, the thermophilic Dps-Te protein is less stable than the L. innocua protein ... nature of the residues lining the openings on the protein surface and protein cavity (Table 2). The size variation is not surprising because these pores are for...

Ngày tải lên: 16/03/2014, 12:20

... 2D [ 15 N, 1 H]-HSQC spectrum indicates that the protein is prone to aggregation (Fig. 5A). The absence of a globular fold is supported by the small dispersion of the amide proton chemical shifts (Fig. ... homology among fish species is 60% among the PrP1 proteins, 50% among the PrP2 proteins, and 40% between PrP1s and PrP2s [8], one is tempted to hypothesize that with...

Ngày tải lên: 30/03/2014, 04:20

... [3,6]. This raises the possibility that the secretory granule target- ing of the PC enzymes is mediated by interaction of the helices in their C-termini with specific membrane domains in the secretory ... Note the relative staining distribution of the fusion proteins between the TGN (open arrows), the cytoplasmic region, and the granule-containing cytoplasmic exte...

Ngày tải lên: 18/02/2014, 16:20

... sufficient for docking of the peptide into the ACE2 active site. In fact, the distance of this positive charge from the surface of the binding pocket is also crucial. Role of His505 and His345 in catalysis Sequence ... of the catalytic histidines in both ACE and thermolysin, sug- gesting that His505 is the catalytic histidine of ACE2. On the basis of these features, we...

Ngày tải lên: 20/02/2014, 01:20

... supporting the existence of a protease that cleaves s-DAPK-1 protein in vivo. The reason why the cleavage band was not observed in this assay may be the rapid degradation of the purified protein from the cell ... full- length s-DAPK-1, and that the cleavage signal is within the C-terminal tail extension. This is further suggested by the in vitro cleavage assay, in...

Ngày tải lên: 18/02/2014, 17:20

... One of these is the subfamily comprising Axl, Sky and Mer (as we shall refer to them hereafter), also referred to as the TAM family (Tyro3, Axl and Mer). This RTK subfamily is defined by a combination ... of the receptor tyrosine kinases Axl, Sky and Mer. Furthermore, a hallmark of the Gas6–Axl system is the unique ability of Gas6 and pro- tein S to tether their non receptor-...

Ngày tải lên: 19/02/2014, 05:20

... response to hypoxia, but this natural response is not sufficient to prevent the further progression of heart disease [9–11]. It is therefore important to understand the mecha- nisms of VEGF regulation ... fractions 6 and 7, was abolished by the V19 mutation, verifying the nature of these complexes (Fig. 5). These data indicate that the CCV1 complex is preformed in solution....

Ngày tải lên: 19/02/2014, 12:20

... not shown). The precise regiospecificity of the activity encoded by TpdesB was further investigated by comi- gration with authentic standards for desaturated dihydroxy-LCBS. This indicated that the novel ... towards sphinganine was reported from Aquilegia vulgaris [24]. The introduction of the D 8 -desaturation into dihydroxy- lated substrates may represent the first step in...

Ngày tải lên: 20/02/2014, 01:20

... protect the fish to the freezing point of seawater [13,14]. The main reason this isoform has escaped detection for 30 years is that it is very thermolabile and denatures at room temperature. This discovery ... change in the signal as the sample was warmed from 13 to 30 °C. To investigate whether the denaturation of this extremely thermolabile protein is reversible, CD was...

Ngày tải lên: 20/02/2014, 01:20