... concentration of 0.38 lm withincubation (37 °C) for 1, 5, 10 , 15 , 30, 45, 60 and 12 0 min.Different concentrations of bicistronic P450 4X1 protein were used (0.075, 0.38, 0.75, 1. 13 and 1. 50 lm) with incuba-tion ... at rates of 8–386 pmolÆmin )1 Æmg )1 protein. Mouse brain microsomes produced only two products,distinct from the liver products, at rates of 7 and 17 pmolÆmin )1 Æmg )1 protein. None of the productswere ... http://npsa-pbil.ibcp.fr) which indicated thepresence of two a-helix structures in the N-terminalpart of the protein (1 11 and 15 –44). N-Terminal-modified P450 4X1 constructs 3 and 4 (Table 1) werebased on...