Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx
Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx
... 2007 FEBS 16 65 Expression and physiological role of CCN4⁄ Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes Takeshi Yanagita 1, 2 , Satoshi Kubota 1 , Harumi Kawaki 1 , Kazumi ... EF0259 21, EF025922 and EF025923 (Received 20 November 2006, revised 15 January 2007, accepted 19 January 2007) doi :10 .11 11/ j .17 42-4658.2007.05709.x C...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide pdf
... concentration of 0.38 lm with incubation (37 °C) for 1, 5, 10 , 15 , 30, 45, 60 and 12 0 min. Different concentrations of bicistronic P450 4X1 protein were used (0.075, 0.38, 0.75, 1. 13 and 1. 50 lm) with incuba- tion ... at rates of 8–386 pmolÆmin )1 Æmg )1 protein. Mouse brain microsomes produced only two products, distinct from the liver products, at rates of 7...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt
... prolifera- tive and differentiation state, basal level of ERK1 ⁄ 2 and Akt kinase phosphorylation, and P2Y 1 and P2Y 12 receptor protein expression and functional activity. Examination of the effects of specific ... characterized by increased expression of the P2Y 12 receptor and low expression of the P2Y 1 recep- tor. In cells grown in medium containi...
Ngày tải lên: 30/03/2014, 08:20
Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
... IANBD bands, indicative of covalent binding of the dyes [19 ]. Binding activities of Ag-NPA -1 Binding of fatty acids, retinol and DAUDA were studied by changes in the intrinsic Trp fluorescence of ... emission and FRET from the Trp residue, buried in the interior of the protein molecule, to the ionone ring of retinol would be expected. Inter- estingly, binding of re...
Ngày tải lên: 16/03/2014, 18:20
Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... upregulation of pro-IL-1b and that processing of pro-IL-1b into mature IL-1b requires activating cleavage of pro-caspase 1 [23], the cleavage of both pro-IL-1b and pro-caspase 1 was examined in hypox- ia ... promote stimulus-induced secretion of IL -1 beta and IL -18 in human blood. J Immunol 16 5, 4 615 –4623. 31 Mehta VB, Hart J & Wewers MD (20 01) ATP-stimul...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc
... activity of the PX domains of Noxo1b and Noxo1c. The GST-fusion proteins of Noxo1b-PX (amino acids 1 15 3) (A) and Noxo1c-PX (amino acids 1 15 8) (B) were tested in an overlay lipid-binding assay using ... (PX) domain in the N-terminus. PX domains occur in a variety of proteins involved in cell signaling, mem- brane trafficking, and polarity establishment, and func-...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt
... 22.7 Number of reflections used in the refinement 62307 12 4549 14 4860 12 1622 79506 11 7097 18 0 614 216 172 10 6579 Number of nonhydrogen atoms 12 722 12 825 13 012 12 9 41 9492 12 929 13 003 13 0 71 12804 Mean ... of C163, thirdly the degree to which the enzyme stabilizes the acyl–enzyme complex, and finally the WT K328R K328H 0 .14 0 1. 110 .14 0.550. 015 0 .14 0.5...
Ngày tải lên: 19/02/2014, 08:20
Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... co-expressing dopamine D 1 and D 2 fusion pro- teins (D 1 MUT–CFP – genetic variant of dopamine D 1 receptor and D 2 –YFP). g Measured in cell co-expressing dopamine D 1 and D 2 fusion proteins (D 1 MUT–CFP ... expressing CFP coupled to the dopamine D 1 receptor. b Measured in cell co-expressing dopamine D 1 and D 2 fusion proteins (D 1 –CFP and D 2 –YFP). c Me...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... ND D142N 0.26 ± 0.07 0.28 ± 0.04 0. 31 ± 0.05 5.4 ± 1. 2 4 .1 ± 2.0 16 .6 ± 5.3 E144Q 0.0039 ± 0.0007 0.0043 ± 0.0005 0.0 01 ± 0.0002 14 .7 ± 5.4 19 .1 ± 4.2 13 .4 ± 4.2 Y 214 F 0 .11 7 ± 0. 014 0 .11 7 ± 0. 011 ... activity nmolÆs )1 Æmg )1 %ofWT Y10F 7.9 ± 0.5 5.7 S93A 7.7 ± 0.4 5.6 D140N 0 .17 ± 0.04 0 .12 D140A 0 .17 ± 0. 01 0 .12 D142N 4.4 ± 0.5 3.2 D142A 0 .13 ± 0. 01 0...
Ngày tải lên: 07/03/2014, 14:20
Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... 290 Ser 16 2 Asp 214 Asp 214 Asp 212 Asp 212 Asp1 61 Asp1 61 Arg 213 Arg 213 Phe 219 Phe 219 Trp222 Trp222 Trp92 Trp92 Gly 91 Gly 91 Gly90 Gly90 Gly89 Gly89 Gly2 91 Gly2 91 T y r292 T y r292 Tyr190 Tyr190 Tyr190 Tyr190 His290 His290 His88 His88 Ser162 Ser162 Rv1399c ... h of IPTG induction (Fig. 1) . Purification and refolding of Rv1399c Given the large quantity of insol...
Ngày tải lên: 07/03/2014, 16:20