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Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx

Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx

Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx

... 2007 FEBS 16 65 Expression and physiological role of CCN4⁄ Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes Takeshi Yanagita 1, 2, Satoshi Kubota 1 , Harumi Kawaki 1 , Kazumi ... EF0259 21, EF025922 and EF025923(Received 20 November 2006, revised 15 January 2007, accepted 19 January 2007)doi :10 .11 11/ j .17 42-4658.2007.05709.xCCN4 ⁄ Wnt-induced secreted protein 1 (WISP1) is ... FEBS 16 57CCN4 ⁄ WISP1 and WISP1v proteins were actuallytranslated from CCN4 ⁄ WISP1 and WISP1v mRNAs in HCS-2 ⁄ 8 cells.A rabbit ortholog of the reported CCN4/WISP1- splicing variant (WISP1v)...
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Báo cáo khoa học: Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide pdf

Báo cáo khoa học: Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide pdf

... concentration of 0.38 lm withincubation (37 °C) for 1, 5, 10 , 15 , 30, 45, 60 and 12 0 min.Different concentrations of bicistronic P450 4X1 protein were used (0.075, 0.38, 0.75, 1. 13 and 1. 50 lm) with incuba-tion ... at rates of 8–386 pmolÆmin )1 Æmg )1 protein. Mouse brain microsomes produced only two products,distinct from the liver products, at rates of 7 and 17 pmolÆmin )1 Æmg )1 protein. None of the productswere ... http://npsa-pbil.ibcp.fr) which indicated thepresence of two a-helix structures in the N-terminalpart of the protein (1 11 and 15 –44). N-Terminal-modified P450 4X1 constructs 3 and 4 (Table 1) werebased on...
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Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt

Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt

... prolifera-tive and differentiation state, basal level of ERK1 ⁄ 2 and Akt kinase phosphorylation, and P2Y 1 and P2Y 12 receptor protein expression and functional activity.Examination of the effects of specific ... characterized by increased expression of theP2Y 12 receptor and low expression of the P2Y 1 recep-tor. In cells grown in medium containing 10 % (v ⁄ v)fetal bovine serum, the expression ratio of the ... the otherhand, Akt phosphorylation increased in parallel with the low expression of the P2Y 1 receptor, indicating the inhibitory role of P2Y 1 in Akt pathwaysignaling. The shift in nucleotide...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx

Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx

... IANBD bands,indicative of covalent binding of the dyes [19 ].Binding activities of Ag-NPA -1 Binding of fatty acids, retinol and DAUDA were studiedby changes in the intrinsic Trp fluorescence of ... emission and FRET from the Trpresidue, buried in the interior of the protein molecule,to the ionone ring of retinol would be expected. Inter-estingly, binding of retinol caused an increase of theemission ... September2004)doi :10 .11 11/ j .14 32 -10 33.2004.04398.xAg-NPA -1 (AgFABP), a 15 kDa lipid binding protein (LBP) from Ascari-dia galli, is a member of the nematode polyprotein allergen/antigen (NPA)family....
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

... upregulation of pro-IL-1b and that processing of pro-IL-1b into mature IL-1b requires activatingcleavage of pro-caspase 1 [23], the cleavage of bothpro-IL-1b and pro-caspase 1 was examined in hypox-ia ... promotestimulus-induced secretion of IL -1 beta and IL -18 in human blood. J Immunol 16 5, 4 615 –4623. 31 Mehta VB, Hart J & Wewers MD (20 01) ATP-stimulatedrelease of interleukin (IL)-1beta and IL -18 requirespriming ... pro-IL-1b and TNF-a expression in MSCs in the presence and absence of BAY 11 -7082 or MG132. *, nonspecificband. (C) Representative western blots forpro-IL-1b expression in MSCs in the pres-ence and...
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Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc

Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc

... activity of the PX domains of Noxo1b and Noxo1c. The GST-fusion proteins of Noxo1b-PX (amino acids 1 15 3) (A) and Noxo1c-PX (amino acids 1 15 8) (B) were tested in an overlay lipid-binding assay using ... (PX)domain in the N-terminus. PX domains occur in avariety of proteins involved in cell signaling, mem-brane trafficking, and polarity establishment, and func-tion as phosphoinositide-binding modules ... Nox1 in co-operation with Noxo1 [15 ,17 19 ], was also expressed(Fig. 2F) in LNCaP, PC3, and DU145 cells, suggestingthat Nox1 is regulated by Noxo1c and Noxa1 in thesecancer cells. The role of...
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Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... 22.7Number of reflectionsused in the refinement62307 12 4549 14 4860 12 1622 79506 11 7097 18 0 614 216 172 10 6579Number of nonhydrogen atoms 12 722 12 825 13 012 12 9 41 9492 12 929 13 003 13 0 71 12804Mean ... of C163, thirdly the degree to which the enzymestabilizes the acyl–enzyme complex, and finally theWTK328RK328H0 .14 0 1. 110 .14 0.550. 015 0 .14 0.550.55 1. 11 µg KAS 15 4 311 1028769C# 12 18 :1 14 16 18 MFig. ... 8.70.8Mal-ACPAc-ACP 15 4 311 1 210 28769AµMWT30K328H30K328R30C163A 10 min 10 B8.73.6 4.32.2 4.37 .1 2.2 2.24.3 8.7 8.70Mal-ACPAc-ACP 13 154 311 1 210 28769µMK328I K328EK328AK328F 1. 8Fig. 7. Decarboxylation...
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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf

Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf

... co-expressing dopamine D 1 and D2fusion pro-teins (D 1 MUT–CFP – genetic variant of dopamine D 1 receptor and D2–YFP).gMeasured in cell co-expressing dopamine D 1 and D2fusion proteins (D 1 MUT–CFP ... expressing CFP coupled to the dopamine D 1 receptor.bMeasured in cell co-expressing dopamine D 1 and D2fusion proteins (D 1 –CFP and D2–YFP).cMeasured in cellco-expressing dopamine D 1 and ... multiple sites of interaction, including anintermolecular interaction involving transmembranedomain 4. Biochemistry 42, 11 023 11 0 31. 20 Nemoto W & Toh H (2005) Prediction of interfaces foroligomerizations...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

... NDD142N 0.26 ± 0.07 0.28 ± 0.04 0. 31 ± 0.05 5.4 ± 1. 2 4 .1 ± 2.0 16 .6 ± 5.3E144Q 0.0039 ± 0.0007 0.0043 ± 0.0005 0.0 01 ± 0.0002 14 .7 ± 5.4 19 .1 ± 4.2 13 .4 ± 4.2Y 214 F 0 .11 7 ± 0. 014 0 .11 7 ± 0. 011 ... activitynmolÆs )1 Æmg )1 %ofWTY10F 7.9 ± 0.5 5.7S93A 7.7 ± 0.4 5.6D140N 0 .17 ± 0.04 0 .12 D140A 0 .17 ± 0. 01 0 .12 D142N 4.4 ± 0.5 3.2D142A 0 .13 ± 0. 01 0.094E144Q 0.028 ± 0. 015 0.023E144A 0.0 016 ± 0.0003 ... 8.43 1E15cD142N, Asn142 ÔupÕcD142N <0.0 Absent 6.74 1E15 D 215 N D 215 N <0.0 12 .4 5.75 1E6N Q144E WT <0.0 >20.0 11 .76 1E6N Q144E, no substrate WT <0.0 15 .2 7.97 1E6N Q144E,...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... 290Ser 16 2Asp 214 Asp 214 Asp 212 Asp 212 Asp1 61 Asp1 61 Arg 213 Arg 213 Phe 219 Phe 219 Trp222 Trp222Trp92Trp92Gly 91 Gly 91 Gly90Gly90Gly89Gly89Gly2 91 Gly2 91 Tyr292 Tyr292Tyr190 Tyr190Tyr190Tyr190His290His290His88His88Ser162Ser162Rv1399c ... h of IPTG induction (Fig. 1) .Purification and refolding of Rv1399cGiven the large quantity of insoluble material obtained [upto 16 0 mg of protein from 6 L of cell culture (Table 1, Fig. 1 lane ... [23].Results and DiscussionCloning and expression of Rv1399cThe Rv1399c gene product encodes a 318 amino acid protein with a molecular mass of 31. 7 kDa and acalculated pI of 4.53 and belongs...
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