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Báo cáo khoa học: Mapping of the functional phosphate groups in the catalytic core of deoxyribozyme 10–23 potx

Báo cáo khoa học: Mapping of the functional phosphate groups in the catalytic core of deoxyribozyme 10–23 potx

Báo cáo khoa học: Mapping of the functional phosphate groups in the catalytic core of deoxyribozyme 10–23 potx

... modification on the catalytic activity of deoxyribozyme 10–23 The functional role of the individual phosphate groups in the catalytic core of deoxyribozyme 10–23 wasexamined by determination of the thio ... deoxyribozyme 10–23 In order to obtain a deeper insight into the functional role of the oxygen atoms of the P5 phosphate group in the catalytic core of deoxyribozyme 10–23, we pre-pared two PS deoxyribozymes ... of nucleoside in position 6 of the catalytic core We were interested in whether there are any other lig-ands in the 10–23 catalytic core that might be directlyinvolved in stabilization of the...
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Báo cáo khoa học: Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor ppt

Báo cáo khoa học: Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor ppt

... receptor–ligandinteractions of the histamine receptors. Elucidation of the structure and dynamics of membrane proteins is a challen-ging task essential for proper understanding of the func-tioning of fundamental ... [28]. The N-terminal sequence of the H1 receptor indeed containstwo N-linked glycosylation consensus sites, but in prelim-inary studies no binding to concanavalin A was observed,indicating that ... fitting procedure to determine total andnonspecific binding at the same time. In saturation bindingexperiments the experimentally determined nonspecificbinding was used to estimate nonspecific binding...
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Tài liệu Báo cáo khoa học: An immunomodulator used to protect young in the pouch of the Tammar wallaby, Macropus eugenii pptx

Tài liệu Báo cáo khoa học: An immunomodulator used to protect young in the pouch of the Tammar wallaby, Macropus eugenii pptx

... production of inter-leukins and secretion of immunoglobulins on the mucosal surface of the intestine [49,50]. The antibacte-rial defence of the intestinal mucosa depends in parton stimulation of CCK ... euge-nin, indicating that eugenin is indeed acting throughCCK2receptors.To further investigate the interaction of eugenin withCCK2receptors, we investigated the effect of eugeninon lymphocyte ... fractioncontaining eugenin is indicated by an asterisk in Fig. 2.Two further HPLC separations of the initial eugenin frac-tion (10–75% acetonitrile over a period of 60 min at a flowrate of 1 mLÆmin)1.)...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Using adaptor grammars to identify synergies in the unsupervised acquisition of linguistic structure" docx

... terminal in the grammar. If Ais a terminal then GAis the distribution that puts all of its mass on the unit tree (i.e., tree consisting of asingle node) labeled A. If A is a nonterminal thenGAis ... parse of thatstring with a PCFG approximation to the adaptorgrammar. This PCFG contains a production for eachadapted subtree in the parses of the other strings in the training corpus. A final ... shown in Figure 1. In this grammar and the grammars below, under-lining indicates an adapted nonterminal. Phonemeis a nonterminal that expands to each of the 50 dis-tinct phonemes present in the...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

... that the RPEL domain of MRTF-A bindsactin more strongly than the RPEL domain of myocar-din, and that the RPEL motif itself is an actin-bindingelement. RPEL1 and RPEL2 of myocardin bind actinweakly ... asSTARS-interacting proteins. These novel proteins con-tain four LIM domains and a C-terminal villin head-piece domain, which mediates actin-binding in severalproteins, such as villin and dematin ... cell lines [94].Table 1. Role of nuclear actin-binding proteins interacting with the androgen receptor. AR, androgen receptor; LBD, ligand-binding domain.Actin-bindingproteinTargetingsequence...
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Báo cáo khoa học:

Báo cáo khoa học: "Some Comments on Algorithm and Grammar in the Automatic Parsing of Natural Languages" ppt

... written in the form of appropriate tables of rules. The paper raises the question of the increasing complexity of the tables when more than the most elementary natural-language con- ditions are included, ... machine translation. It must be noted at the outset that in this author’s opinion the aim of the automatic-parsing component of a machine-translation program is the adequate recognition of the ... Examples of this are very common in English and include many of the frequently cited instances of nesting. The second consideration is that the determination of external functioning by con-...
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Báo cáo khoa học: A novel serine protease highly expressed in the pancreas is expressed in various kinds of cancer cells potx

Báo cáo khoa học: A novel serine protease highly expressed in the pancreas is expressed in various kinds of cancer cells potx

... confirm the expression of proseminmRNA in some cell lines. A band of the predicted size(500 bp) of the PCR product was observed for most of the cell lines tested, including HPC-YO and HPC-YS, in ... 31 000, indicating successfuldigestion of the artificial trypsinogen pro-enzyme frag-ment. The amount of 31 kDa prosemin increased in pro-portion to the time of incubation with enterokinase.Prosemin ... cancer cell lines.Ovarian cancers express some kinds of chromosome 16serine proteases, including testisin and prostasin. Weinvestigated the expression of prosemin in ovarian can-cers using immunohistochemistry....
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Báo cáo khoa học: FLIP and MAPK play crucial roles in the MLN51-mediated hyperproliferation of fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis pdf

Báo cáo khoa học: FLIP and MAPK play crucial roles in the MLN51-mediated hyperproliferation of fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis pdf

... critical role in the proliferation of MH7A cells. In order to determine whether MLN51 is involved in the anti-apoptosis as well as cell proliferation of MH7A in response to GM-CSF, we examined the apoptosis ... angiogenesis in arthriticjoints [2–4]. Several cytokines and RA factors existing in the RA environment stimulate the overgrowth of FLSs, leading to the aggravation of disease. Amongstthese factors, ... followed by the blocking of FLS apoptosis.FLIP upregulated by MLN51 plays a crucial role in the anti-apoptosis of MH7A cellsWe examined whether FLIP was involved in the anti-apoptosis of MH7A...
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Báo cáo khoa học: Functionally distinct dopamine and octopamine transporters in the CNS of the cabbage looper moth* potx

Báo cáo khoa học: Functionally distinct dopamine and octopamine transporters in the CNS of the cabbage looper moth* potx

... dopamine-selective transporter is found.Keywords: insect; neurotransmitter; transporter; dopamine;octopamine; cocaine. The catecholamine dopamine (DA), the phenolamineoctopamine (OA) and the indolamine ... does the moth OAT substitute for DAT in dopami-nergic signaling pathways in the moth CNS, and what is the nature of the mechanism that substitutes for OAT in the octopaminergic pathways in the ... to TrnDAT, the activity of TrnOAT is consid-erably less sensitive to blockers of monoamine uptake in mammals, such as the plant alkaloid cocaine. The mode of action of cocaine in the lepidopteran...
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Báo cáo khoa học: Upstream and intronic regulatory sequences interact in the activation of the glutamine synthetase promoter pot

Báo cáo khoa học: Upstream and intronic regulatory sequences interact in the activation of the glutamine synthetase promoter pot

... (muscle). The application of the ANOVAtechnique allowed us to segregate the main(intrinsic) activities of the respective elements from the effects of their interactions. Using this approach, the upstream ... sequences present in the respective constructsare shown in the left portion of the figure as solid black lines. The upper line shows the linkage of the 5.3-kb genomic GS segment with the luciferasereporter ... protein concentration (bicinchoninic acid reagent;Pierce).Splicing of modified first intronConstructs carrying the different modules of the GS firstintron were tested for proper splicing of the...
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