Báo cáo khoa học: Sherlock Holmes and the proteome ) a detective story Pier Giorgio Righetti1 and Egisto Boschetti2 potx

... the major advantages as well as the limitations of the present approach. The advantages are at least twofold: while this highly diversified ligand library is able to greatly concentrate rare and ... amenable to analysis by MS and any other analytical tool. Exam- ples are given of analysis of human urine and serum, as well as cell and tissue lysates, such as Escherichia coli an...

Ngày tải lên: 07/03/2014, 10:20

... Suzuki A, Kobayashi T, Paul-Prasanth B, Lau EL, Hamaguchi S, Sakaizumi M & Nagahama Y (200 7) DMY gene induces male development in genetically female (XX) medaka fish. Proc Natl Acad Sci USA 104, ... Natl Acad Sci USA 99, 11778–11783. 9 Matsuda M, Nagahama Y, Shinomiya A, Sato T, Matsuda C, Kobayashi T, Morrey CE, Shibata N, Asakawa S, Shimizu N et al. (200 2) DMY is a Y-spe- ci...

Ngày tải lên: 14/02/2014, 19:20

... 5′3′ CCUUUAGGGACCGUUACACUA CCUUUAGGGACCGUUACACUA CCUUUAGGGACCGUUACACUA ACAAUGUGAAA GCAAUGUGAUA 5′ 3′ UAAAUGUGAAU ACUAAGAGUAA GCAAUGUGAUA IL6R mRNA mut 1 5′ 3′ UAAAUGUGAAU ACAAUGUGAAA GCUAAGAGUUA IL6R ... through the MTT (A) and colony formation (B) assays (*P < 0.0 5). 2521 5′ 3′ 5′ 3′ 5′ 3′ 5′ CCUUUAGGGACCGUUACACUA CCUUUAGGGACCGUUACACUA CCUUUAGGGACCGUUACACUA 3′ UAAAUG...

Ngày tải lên: 18/02/2014, 04:20

... Acta 1269, 105–114. 38. Agarwal, M.B., Gupte, S.S., Viswanathan, C., Vasandani, D., Ramanathan, J., Desai, N., Puniyani, R.R. & Chablani, A. T. (199 2) Long-term assessment of efficacy and safety ... Australia), penicillin and gluta- mine from Gibco BRL (Auckland, New Zealand) and streptomycin sulphate from Calbiochem (La Jolla, USA). The synthetic medium, Ultroser G was purchas...

Ngày tải lên: 20/02/2014, 11:20

... 5¢-GAAAAGCAAGAGTTACCCCC GCGTTTATGGCACAGGAAG 5¢-CTTCCTGTGCCATAAAC GCGGGGGTAACTCTTGCTTTTC N43 5A Between SOL and M3 5¢-GCTTGAGTTCCAG GCTGTGAGCATCAATGGAGATAAGTATC 5¢-GATACTTATCTCCATTGATGCTCACA GCCTGGAACTCAAGC S43 7A ... 5¢-GCTTGAGTTCCAG GCTGTGAGCATCAATGGAGATAAGTATC 5¢-GATACTTATCTCCATTGATGCTCACA GCCTGGAACTCAAGC S43 7A Between SOL and M3 5¢-GGCTTGAGTTCCAGAATGTG GCCATCAATGGAGATAAG 5¢-CTTATCT...

Ngày tải lên: 16/03/2014, 06:20

... Sa ´ nchez-Bautista, Andris Kazaks*, Melanie Beaulande, Alejandro Torrecillas, Senena Corbala ´ n-Garcı ´ a and Juan C. Go ´ mez-Ferna ´ ndez Departamento de Bioquı ´ mica y Biologı ´ a Molecular, Universidad ... 1700– 1600 cm )1 interval (amide I) having identical positive and negative area. 2D correlation analysis was carried out using the 2d- pocha program written by D. Adach...

Ngày tải lên: 16/03/2014, 13:20

... in the GAL4 activation domain plasmid pGAD10. The dematin bait and the library was transformed into CG-1945 and plated on media lacking the amino acids tryptophan, leucine, and histidine in the ... growth was designated by ( ) while failure to activate the L acZ reporter gene was designated as (W). (B) Yeast mating between dematin an d Ras-GRF1 and between limatin and...

Ngày tải lên: 17/03/2014, 11:20

... dithionite, the Soret peak was blue-shifted from 418 to 412 nm, accompanied by a decrease in intensity, whereas the Q band (a collective term for the a and b bands), with an a- band peak at 570 nm and a ... encapsulated PGIS interacted with the heme ligands. We chose U46619 (an O-based ligand; a substrate analog whose oxygen atom at the C9 position is replaced with a...

Ngày tải lên: 23/03/2014, 07:20

... PAGE, the peak comprised all of the subunits from the enzyme (a, b, and c) and the reactivase (a and b) (data not shown). It was thus evident that this peak contained the enzymeÆreactivase complex(es) ... nondenaturing PAGE, the bands of the enzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivase complexes (upper and l...

Ngày tải lên: 15/02/2014, 01:20

... Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al. (200 3) Genome sequence of Bacillus cereus and comparative analysis with ... G AB catalysis have been identified to date [12] which are based either on a single, bifunc- tional GAB catalyst or on a GAB catalysts pair. The available biochemical data on M...

Ngày tải lên: 19/02/2014, 00:20