Báo cáo khoa học: Docosahexaenoic acid stabilizes soluble amyloid-b protofibrils and sustains amyloid-b-induced neurotoxicity in vitro ppt

... FEBS Docosahexaenoic acid stabilizes soluble amyloid-b protofibrils and sustains amyloid-b- induced neurotoxicity in vitro Ann-Sofi Johansson 1 , Anita Garlind 2 , Fredrik Berglind-Dehlin 1 ,Go ¨ ran ... aggregation in vitro, stabilizing soluble intermediates, i.e. protofibrils, and thereby maintaining Ab-induced toxicity in PC12 cells. Results Unsaturated fatty...

Ngày tải lên: 07/03/2014, 10:20

... changes. Unfilled and filled arrowheads indicate the 37 amino acid residues involved in DNA binding activity. Filled arrowheads correspond to the seven amino acid residues involved in DNA binding activity ... mixture. Incubation continued for other 10 min at 4 °C. Mutations introduced in TATTT AAA box are marked in small letters and deletions are in dashes in the oligonucleo...

Ngày tải lên: 16/03/2014, 18:20

... stroma and energy (i.e. as in Fig. 1B,C). Fig. 5. In vitro integrated Hcf106 and cpTatC assemble into 700-kDa complexes that bind twin arginine containing precursors. (A,B) Precursor binding to ... 7). Twin arginine precursor binding by in vitro assembled  700 kDa complex As a first test of the functionality of in vitro inserted components, we examined the ability of complex...

Ngày tải lên: 30/03/2014, 20:20

... the Sox9-binding sites of the AP-2e promoter (Sox9_1 and Sox9_2). Incubation of in vitro- synthesized Sox9 with the labeled oligonucleo- tides containing the Sox9-binding sites resulted in a strong ... PCR using specific primer pairs generating frag- ments spanning the first (Sox9_1) or the second (Sox9_2) Sox9-binding site of the AP-2e promoter. Sox9 binding to both binding sites (Sox9_...

Ngày tải lên: 18/02/2014, 08:20

... by incubating sections in 0.3% hydrogen peroxide in methanol for 30 min at room temperature. Slides were then washed and incubated in a blocking solution accord- ing to the manufacturer’s instructions. ... Crassostrea gigas [5]. Interestingly, this protein, named C. gigas chitinase-like protein 1 (Cg- Clp1) was found to be involved in the control of growth and remodelling processe...

Ngày tải lên: 19/02/2014, 00:20

... senses and domains. In addition, we apply an automatically gener- ated encyclopedia to a question answering system targeting the Japanese Information- Technology Engineers Examination. 1 Introduction Reflecting ... pages containing a term in question, and uses linguistic ex- pressions and HTML layouts to extract fragments de- scribing the term. They also use a language model to disca...

Ngày tải lên: 20/02/2014, 18:20

... 2009) doi:10.1111/j.1742-4658.2009.06979.x Interaction between acidic activation domains and the activator-binding domains of Swi1 and Snf5 of the yeast SWI ⁄ SNF chromatin remodeling complex has previously been characterized in vitro. ... The in vivo validation of the Swi1 and Snf5 activator-bind- ing domains is also of interest in relation to the two- step binding mechanism bet...

Ngày tải lên: 07/03/2014, 00:20

... induce an in ammatory process in hRE In these experiments using haematoxylin ⁄ eosin-stained reconstructed epidermis (Fig. 7), we noticed that skin thickness increased with time of culture, indicating ... has been reported in a number of cell types, including skin cells. The major HSPs expressed in the skin [5] are Hsp70 (both cognate and inducible forms) [6] and Hsp27 (expressed...

Ngày tải lên: 07/03/2014, 11:20

... encoding: (C) cerulean-elongin B and elongin C- citrine; (D) elongin B-cerulean and elongin C-citrine; (E) cerulean- elongin B and citrine-elongin C; and (F) elongin B-cerulean and citrine-elongin ... detected in the fol- lowing donor-acceptor combinations in decreasing order: cerulean-elongin B and citrine-elongin C > cerulean-elongin B and elongin C-citrine  elongin B-...

Ngày tải lên: 16/03/2014, 05:20

... shown). Catabolism of L -proline and L -arginine does not lead to induction of the alc genes L -Proline and L -arginine can serve as sole carbon and nitrogen sources for A. nidulans and both are catabolized via L -D 1 -pyrroline-5-carboxylate, ... These three amino acids do not induce the alc genes while, as expected, L -proline and L -arginine do induce the prnD gene. This was also...

Ngày tải lên: 17/03/2014, 10:20