Báo cáo khoa học: Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives ) a screening based on affinity for the UMP-CMP binding site potx

... phosphonate analogs that is based on the affinity of the acceptor -binding site of the human UMP-CMP kinase. Several uracil vinylphosphonate derivatives had affinities for human UMP- CMP kinase similar ... Authors Journal compilation ª 2007 FEBS Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives ) a screening based...

Ngày tải lên: 07/03/2014, 09:20

... In the seconds time range there is a final slow radical decay. This reaction was always second- order representing a radical–radical recombination reaction (e.g. dimerization of TyrO Æ radical). ... protein concentration an average second-order rate constant of (9.5 ± 0. 9) · 10 8 LÆmol )1 Æs )1 (25 °C) for TEWL was calculated. Similar second-order rate constants were obtain...

Ngày tải lên: 17/03/2014, 10:20

... initia- tion of the global conformational transition; and (c) to gain some insight into how the local torsional stresses at the contact residues in the super-open state propa- gate through the ... cosub- strate (MgATP 2) ) and thus catalysis. Moreover, the maximal activation of rat liver glucokinase by Glc and the related overall conformational transition, as fol- lowed...

Ngày tải lên: 30/03/2014, 04:20

... groups of ATP are necessary for tetramerization, and that kinetics and tetramerization are closely related. Furthermore, the enzyme concentration was found to have a pivotal effect on catalytic ... conditions with 100 l M dThd. The molecular markers (vertical bars) are (from left to right): b-amylase (200 kDa), BSA (66 kDa), ovalbumin (45 kDa), carbonic anhydrase (30 kDa) and cy...

Ngày tải lên: 18/02/2014, 12:20

... at a conserved cleavage site. Host cell CyPB facilitates the L2 conforma- tion changes. ( 3) These events may induce an additional conformational change that either reduces the affinity of capsids ... addition to the aforementioned conformational changes, the reported transport along filopodia towards the cell body prior to internalization may contribute to the delayed...

Ngày tải lên: 23/03/2014, 04:20

... categories. Table 2 shows the unigram, bigram, and trigram- based average κ between the two human annotators (Human1 , Human2 ). These results suggest that human annotators can reach substantial agreement when ... 2003; Nenkova and Passonneau, 200 4). These evaluation methods assess the information content in the sum- maries that are generated automatically. Finally, rece...

Ngày tải lên: 30/03/2014, 21:20

... Kazusa DNA Research Institute (Chiba, Japan), using a pair of primers (forward, 5¢-CTA GAATT CAACAGCACAGCATGCTGG-3¢; reverse, 5¢-AGAGAA TTCTGCCTGGTTTAAGAGACC-3¢; restriction sites underlined). ... identified. Although calpain 7 contains a C2-like domain, it lacks a penta-EF-hand domain and is classified as an atypical calpain. As one of the significant structural features, mammalian ca...

Ngày tải lên: 18/02/2014, 04:20

... fibrillation: grade 0, no inflammation; grade 1, slight inflammation; grade 2, strong inflammation. The cartilage damage severity of the femoral condyles and tibial plateau was graded from 0 to 4: grade ... flexed. Digital pressure was applied to the medial side of the straight patellar ligament. A 21-gauge spinal needle was inserted through the fat pad into the intercondylar spa...

Ngày tải lên: 18/02/2014, 14:20

... ARTICLE Chemical approaches to mapping the function of post-translational modifications David P. Gamblin, Sander I. van Kasteren, Justin M. Chalker and Benjamin G. Davis Chemistry Research Laboratory, Department ... Lewis X -tetrasaccharide on the surface of the protein. Quantitative conversions can be obtained for the chemical glycosylation and each of these subsequent enzymatic...

Ngày tải lên: 18/02/2014, 17:20

... pro-death proteins, such as cytochrome c, which are released into the cytoplasm as a consequence of apoptotic signals. The release of these apoptotic factors involves alteration of the mitochondrial ... Yethon JA, Epand RF, Leber B, Epand RM & Andrews DW (200 3) Interaction with a membrane sur- face triggers a reversible conformational change in Bax normally associated with...

Ngày tải lên: 19/02/2014, 07:20