Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf

... similar proteinases, CatE and CatD, and can therefore be used to investigate the involvement of these enzymes in antigen processing and presentation. Experimental procedures Enzymes and chemicals CatD ... antibodies were preincubated with different concentrations of CatE or CatD, before standard ELISA. ELISA was performed as described in Experimental pro- cedures. The increasing c...

Ngày tải lên: 07/03/2014, 09:20

... visible absorbance spectra were recorded before and after denaturation of the enzyme with a photometer (model specord 205) from Analytik Jena AS (Jena, Ger- many). All measurements were performed in ... chromatography were verified by native PAGE, in accordance with the standard procedures for SDS-PAGE, using 12.5% separat- ing gels and 5% stacking gels. The gels and the running...

Ngày tải lên: 30/03/2014, 01:20

... Overlap-based edge matching between title and sentence graphs TITLE E J Jaccard-based edge matching between title and sentence graphs D COV E O Overlap-based edge matching between sentence and a document ... nodes having a degree higher than a predefined threshold DEG Average degree for all sentence nodes:  i∈{words(S)} Deg i N GRASE Frequent sentences from bushy paths are...

Ngày tải lên: 07/03/2014, 22:20

... 1. An Identification Tag (t) is appended to the occurrence to indicate the page, sentence, and serial number. Its characters are counted and examined for indications anent its physical make-up. ... therein are capitalized when first encountered in the text. (It is planned to expand this information to include diacritical material designed to aid in the semantic analysis of...

Ngày tải lên: 16/03/2014, 19:20

... sites in the b 4 tetramer are indeed different from the corresponding a1 b1 contact sites in the HbA tetramer. Anyway, we have revealed that over the wide range of pH 5–10, the separated a and ... equations derived therefrom were tested for their fit to the experimental data with the aid of a computer. As a result, the pH-dependence curves for the autoxidation rate of the sepa...

Ngày tải lên: 17/03/2014, 10:20

... multiple seed set learners, a voting schema, and addi- tional features like SuperTags and extra- sentential context. Detailed experiments on hand-annotated data show that our en- hanced algorithm ... word found in WordNet (literal feedback set) and the DoKMIE (nonliteral feedback set). The feedback sets also contain ex- ample sentences provided in the target-word en- tries of thes...

Ngày tải lên: 24/03/2014, 03:20

... such DNA lesions depends on the capacity of the cell to repair the damage before it can be incorporated permanently into the genome. Typically, DNA damage is repaired at a relative high rate in ... formation, HaCaT keratinocytes were irradiated through a quartz plate on which the compound was spread, cells were harvested immediately following irradiation and analysed for pyri- midin...

Ngày tải lên: 07/03/2014, 15:20

... CASE 3,acc and CASE 6,acc are bolded, indicating that w 3 and w 6 are linked and both have the accusative case. The ternary factor CASE-LINK, that connects to these three variable, therefore ... as a graphical model. The variables, all boolean, are represented by circles and are bolded if their correct values are true. Factors are represented by rectangles and are bolded if they fi...

Ngày tải lên: 17/03/2014, 00:20

... the language models we developed to estimate the error-rate. In the experiments reported hereafter, we use the ukWaC+CLC LM to calculate the error-rate. In order to assess the independent as ... shallow, and the approach doesn’t make effi- cient use of the training data as a separate classifier is trained for each grade point. Recently, Chen et al. (2010) has proposed an un- supervi...

Ngày tải lên: 20/02/2014, 04:20

... dimerization domain (aa 321–447), heme domain (aa 519–592), FAD domain (aa 623–728) and NADH domain (residues 744–858) [27]. A unique 17-amino-acid insertion in the molybdopterin-binding domain iden- tified ... (5¢-CATNCCCATNARRTTC CA-3¢) were designed based on the alignment of the deduced amino-acid sequences of nine NR genes from algae and higher plants. The cDNA was used as template...

Ngày tải lên: 07/03/2014, 21:20