Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

... FEBS 4521 In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases Di Ouyang, Long Yi, Liangliang Liu, Hong-Tao Mu and Zhen Xi State Key Laboratory of Elemento-Organic ... pocket, with its amino sugar anchored in the major groove of the DNA pointing toward the 3¢ -bulge- flanking base pair. We were interested in small...

Ngày tải lên: 07/03/2014, 06:20

... and its binding affinity is not altered by the presence of DNA in the DNA binding site. Interestingly, when 3 n M RNA and 10 n M DNA were added simultaneously to a limiting amount of protein in TK8 ... using two different preparations of protein and three different preparations of DNA and RNA substrates. Each time the splicing reaction was minimally affected by...

Ngày tải lên: 17/03/2014, 10:20

... properties of PTOX in vitro. In this report, we produced the protein in E. coli and studied its substrate preferences, the factors in uencing its activity and the nature of suitable metal cofactors and ... stimulated strongly in both membrane types (Fig. 1A,B) in the presence of 0.2 m M of ubiquinone and, to some extent, of dPQ, duroquinone or vitamin K1 (phylloqu...

Ngày tải lên: 31/03/2014, 07:20

... hpf embryos were incubated during 15 min with different kinase inhibitors at 30 °C and then used to analyse in vitro recom- binant CNBP phosphorylation in kinase buffer containing 100 lm ATP and 5 lCi ... November 2006) doi:10.1111/j.1742-4658.2006.05596.x The zinc-finger cellular nucleic acid binding protein (CNBP) is a strikingly conserved single-stranded nucleic acid binding p...

Ngày tải lên: 16/03/2014, 12:20

... dimethylsulfoxide in a concentration-dependent fashion. Our results indicate that in vitro c-secretase cleavage of APP substrates is inhibited by zinc and that zinc increases the apparent molecular mass of ... This is the first report suggesting that this metal binding domain is functional within APP C99 causing oligo- merization with the biochemical consequence of inhib- iting...

Ngày tải lên: 16/03/2014, 23:20

... signal sequence is involved in guiding the protein into the targeting and translocating pathway by interacting with the membrane and other components of the cellular secretory machinery [1]. The signal ... of S. pneumoniae SPase I For in vitro self-cleavage of S. pneumoniae SPase I in the presence of phospholipid, 20 lL of reaction containing 5 lg of purified SPase I...

Ngày tải lên: 23/03/2014, 21:21

... display), increasing the likelihood of correct folding in a redu- cing environment while minimizing the risk of proteo- lytic degradation. The association of plasmid and fusion protein can be maintained ... for 10 min. In vitro selection for mAb M2 binding was by adding 200-lL aliquots of the supernatant to the selection matrix and incubating at room temperature for 30 min....

Ngày tải lên: 30/03/2014, 20:20

... Streptavidin (Invitrogen; 1 : 200) in blocking solution for 1 h. Sections were washed and mounted in Vectashield Mounting medium with 4¢,6-di- amidino-2-phenylindole for nuclear staining (Vector, Burlingame, ... ethanol and in water for 30 min at room temperature. After rinsing twice for 5 min with PBST (NaCl ⁄ P i contain- ing 0.05% Triton X-100), slides were incubated in blo...

Ngày tải lên: 18/02/2014, 08:20

... validated in vitro andusedtoexamine the branch-point kinetics in detail and to obtain insights into the kinetic controls of methionine and threonine synthesis in plants. The branch-point between ... divergence of two fluxes (fluxes of cystathionine and threonine) with the convergence of two fluxes (fluxes of Phser and cysteine). Interestingly, the properties of CGS a...

Ngày tải lên: 20/02/2014, 02:21

... gallium, tin, and zinc, and also variants con- taining meso-protoheme IX, ruthenium meso-protoporphyrin IX and (metal-free) protoporphyrin IX. Engineered haem proteins of this type are of potential ... essentially inactive, and that this is not due to inactivation during isolation of the protein. Light absorption spectra of the purified iron-con- taining and gallium-containin...

Ngày tải lên: 06/03/2014, 11:20