Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot

... 2008)doi:10.1111/j.1742-4658.2008.06540 .x The coagulation factor X activator from Russell’s viper venom (RVV -X) isa heterotrimeric glycoprotein. In this study, its three subunits were cloned and sequenced from the venom gland ... attached to the 3-position of HexNAc of the terminal Hex-HexNAc unit, giving rise to the Le x epitope and SLe x when additionally sialylated. The characteristic D ions for Le x and SLe x were detectedat ... hydrolysis of the peptide bondbetween Arg51 and Ile52 of the heavy chain on factor X, similar to the physiological activation byfactors IXa and VIIa [4,5]. In addition, RVV -X alsoactivates factor IX,...

Báo cáo khoa học: New insights into the P-glycoprotein-mediated efﬂuxes of rhodamines doc

... s.Control experimentsAfter having established the principle of the experiments asexplained above, a set of control experiments was performedin order to further validate the use of the experimentalmodel ... resistantcells. The determination of karequires also the measurement of the rate of passive diffusion of the dye through the plasma membrane. This cannot be done by the simplemeasurement of the increase of ... RhI,IIhyd and Rh B. The aim of the present work was to determine the efﬁciency of the P-gp-mediated efﬂux of these rhodamineswhich can be characterized, as we have shown in the experimental...

Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt

... works 4453 of the S1-bound chloride anion and ‘pushes’ the Cl)out of the bottom of the S1 pocket (the occupancy of the Cl)in this structure is 0.7). The alternate confor-mation of the Asp443 ... with the occupancy of the S1 pocket by a ligand and the ‘open–close’ transition exploits the hinges on the twosides of the lid made up of Asn540 ⁄ Trp541 and Gly548 (GCPII numbering). In the ... highB-factors of the P1 part of the inhibitor, as well as the adjacent S1 residues of GCPIII (compared to the lowerB-factors observed for the P1¢ fragment and the S1¢pocket), suggest that the...

Báo cáo khoa học: New insights into Fragile X syndrome Relating genotype to phenotype at the molecular level docx

... investigations of the I304N mutation play a key role in Fragile X research,there is no consensus in the Fragile X ﬁeld on either the structural or functional consequences of the muta-tion.Because of the ... Cross-sections of ommatidia from eyes of (A), (B) and (C). (Adapted from [20])Fig. 3. Multiple-sequence alignment of KH domains from dFXRP and the human Fragile X proteins (in this ﬁgure named hFMRP, hFXR1,hFXR2). ... males, and premature ovarianfailure in females [2,3]. The syndrome takes its name from the physical‘fragility’ of the chromosome. Chromosome breakageoccurs at the tip of the X chromosome at the...

Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... analyzed and compared with the structures of other complexes of the same family.E-64 binds to these two ervatamins in the same man-ner as that found in other structures of complexes of papain-like ... of mechanism exempliﬁed by a comparison of the cata-lytic-site structure and characteristics of cathepsin B and papain. pH-dependent kinetics of the reactions of cathepsin B from bovine spleen and from ... proton from the catalytic Cys pres-ent at the N-terminus of the helix to the His of the dyad [19,50,51]. The ﬁrst stage of catalysis is mediatedby the highly active thiolate ion of the Cys....

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of subunit 8 and helix a of cytochrome b. The ﬁgure was constructed from the crystal structure of the yeast ... while the remaining subunits of the bc1complex are encoded in the nucleus, synthesized by cytosolicpolysomes, and then imported into mitochondria, therebyreaching their ﬁnal location in the ... °Cand1mMDFP was then added. Afterwards, the cells were mixed with a vortex mixer at maximum speed for10 min at 4 °C. After the further addition of MTE buffer to aﬁnal volume of 50 mL, the...

Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

... The mainchain nitrogens of residues I322 and I303 interact withnitrogen atoms of the adenine ring and the c-carboxylgroup of the side chain of residue D302, the d and x nitrogen atoms of the ... with the x nitrogen of the guanidino group of residue R403 and the c carbonyl oxygen of residue N404. The 3¢-phos-phate is stabilized by interaction of two of its oxygenswith the side-chain oxygen ... tohomogeneity. The kinetic constants for conversion of the substrate oxalylcoenzyme A by the enzyme in the absence and presence of the inhibitorcoenzyme A, as well as in the absence and presence of the activator...

Báo cáo khoa học: Recent insights into cerebral cavernous malformations: animal models of CCM and the human phenotype pptx

... helptranslate the cellular and biochemical insights into a better understanding of disease mechanism. In this minireview, we discuss the contributions of animal models to our growing understanding of the ... biology of cavernousmalformations, including the elucidation of the cellular context of CCMprotein actions and the in vivo conﬁrmation of abnormal endothelial cell–cell interactions. Challenges and ... involvement of multiple cell and tissue types raise the question of where the CCMgenes primarily function, and in which cell type theirloss leads to pathogenesis of disease.Aside from the question of...

Báo cáo khoa học: New insights into evolution of crustacean hyperglycaemic hormone in decapods – ﬁrst characterization in Anomura pdf

... areas of the XO, as a cluster of ﬁve MIH-IR cell bodies wasobserved near the start of the axonal tract, whereas the others were dispersed among CHH-IR structures at the periphery of the XO. Axons ... bernhardus and the squat lobster Gala-thea strigosa. We cloned full-length CHH precursorcDNAs from X- organs of the two species, and analy-sed the peptide content of the sinus glands byMALDI-TOF mass ... (MIH), another member of the CHH family. Accordingly, immunostaining of the X- organ ⁄ sinus glandcomplex of P. bernhardus with heterologous anti-CHH and anti-MIH serashowed the presence of distinct...

Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

... absence of the intracellular and transmem-brane domains does not inﬂuence carboxypeptidase activity of GCPII and neither does the attachment of the His-Xpress epitope at the N-terminus of the 44/750 ... NAAG-hydrolyzing activity (the 44/736 variant), and the C-terminal extension (addition of the V5-His tag inthecaseofthe44/750_V5-Hisvariant)reducedtheactivitybyafactorof>104. Furthermore, C-terminal ... secretion of the truncatedvariants into the culture medium, suggesting the importance of the C-terminus for the correct folding and procession of GCPII throughout the secretory pathway. These data...

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