Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot

... 2008) doi:10.1111/j.1742-4658.2008.06540 .x The coagulation factor X activator from Russell’s viper venom (RVV -X) is a heterotrimeric glycoprotein. In this study, its three subunits were cloned and sequenced from the venom gland ... attached to the 3-position of HexNAc of the terminal Hex-HexNAc unit, giving rise to the Le x epitope and SLe x when add...

Ngày tải lên: 07/03/2014, 06:20

... s. Control experiments After having established the principle of the experiments as explained above, a set of control experiments was performed in order to further validate the use of the experimental model ... resistant cells. The determination of k a requires also the measurement of the rate of passive diffusion of the dye through the plasma membrane. This ca...

Ngày tải lên: 08/03/2014, 08:20

... works 4453 of the S1-bound chloride anion and ‘pushes’ the Cl ) out of the bottom of the S1 pocket (the occupancy of the Cl ) in this structure is 0.7). The alternate confor- mation of the Asp443 ... with the occupancy of the S1 pocket by a ligand and the ‘open–close’ transition exploits the hinges on the two sides of the lid made up of Asn540 ⁄...

Ngày tải lên: 23/03/2014, 05:22

... investigations of the I304N mutation play a key role in Fragile X research, there is no consensus in the Fragile X field on either the structural or functional consequences of the muta- tion. Because of the ... Cross-sections of ommatidia from eyes of (A), (B) and (C). (Adapted from [20]) Fig. 3. Multiple-sequence alignment of KH domains from dFXRP and the...

Ngày tải lên: 30/03/2014, 15:20

... analyzed and compared with the structures of other complexes of the same family. E-64 binds to these two ervatamins in the same man- ner as that found in other structures of complexes of papain-like ... of mechanism exemplified by a comparison of the cata- lytic-site structure and characteristics of cathepsin B and papain. pH-dependent kinetics of the reacti...

Ngày tải lên: 18/02/2014, 16:20

... transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of subunit 8 and helix a of cytochrome b. The figure was constructed from the crystal structure of the yeast ... while the remaining subunits of the bc 1 complex are encoded in the nucleus, synthesized by cytosolic polysomes, and then imported into mitochondr...

Ngày tải lên: 19/02/2014, 12:20

... The main chain nitrogens of residues I322 and I303 interact with nitrogen atoms of the adenine ring and the c-carboxyl group of the side chain of residue D302, the d and x nitrogen atoms of the ... with the x nitrogen of the guanidino group of residue R403 and the c carbonyl oxygen of residue N404. The 3¢-phos- phate is stabilized by interaction...

Ngày tải lên: 06/03/2014, 11:20

... help translate the cellular and biochemical insights into a better understanding of disease mechanism. In this minireview, we discuss the contributions of animal models to our growing understanding of the ... biology of cavernous malformations, including the elucidation of the cellular context of CCM protein actions and the in vivo confirmation of abnormal end...

Ngày tải lên: 15/03/2014, 10:20

... areas of the XO, as a cluster of five MIH-IR cell bodies was observed near the start of the axonal tract, whereas the others were dispersed among CHH-IR structures at the periphery of the XO. Axons ... bernhardus and the squat lobster Gala- thea strigosa. We cloned full-length CHH precursor cDNAs from X- organs of the two species, and analy- sed the peptide...

Ngày tải lên: 23/03/2014, 07:20

... absence of the intracellular and transmem- brane domains does not influence carboxypeptidase activity of GCPII and neither does the attachment of the His- Xpress epitope at the N-terminus of the 44/750 ... NAAG-hydrolyzing activity (the 44/736 variant), and the C-terminal extension (addition of the V5-His tag in thecaseofthe44/750_V5-Hisvariant)reducedtheactivity...

Ngày tải lên: 07/03/2014, 15:20