Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx

... E552 in NBD1 and E1197 in NBD2. To further characterize the role of these residues in catalysis, we created in Abcb1a the single-site mutants E552D, N and A in NBD1, and E1197D, N and A in NBD2, ... further the role of the IC in the catalysis of ABC transporters, we created, in Abcb1a, six single-site mutants (E552D, N and A, and E1197D...

Ngày tải lên: 07/03/2014, 06:20

... to date concerns the role of GPR in enhancing cell proliferation [16–20]. There are also examples suggesting t he antiproliferative activity of GPRs [21–23]. The role of GPR30 in glucocorticoid-mediated ... discrimination of cells differing in their sensitivity to steroids is essential in diseases involving resistance to steroid h ormone treatment. In the present...

Ngày tải lên: 16/03/2014, 18:20

... inhibit growth of the strain even in the presence of IPTG induction. All of these results suggest that the low gene expression associated with NGG codons in the down- stream region following the initiation ... paus- ing at early NGG codons is the reason for the observed low gene expression. The length of the peptidyl moiety of the peptidyl- tRNA in uences t...

Ngày tải lên: 30/03/2014, 20:20

... at the interface between the a and b subunits. A comparison of the amino-acid sequences showed that the ADP-binding motif in the a subunit of PDH1 clearly differs from that in the a subunit of ... stream from the 5¢-terminus of the predic- ted ORF, is the proper initial codon for a2. The N-ter- minal amino-acid sequences of the b2 and c2 subunits corres...

Ngày tải lên: 20/02/2014, 01:20

... N e -(c-glutamyl)lysine bonds, the incorpor- ation of polyamines into proteins, the deamidation of protein-bound glutamines, and the covalent attachment of proteins to lipids such as x-hydroxyceramides ... peptides used exhibited a pNA residue on the C-side. The already slight yellowing of the solution indicated SM-TAP activity against the compound in the incubat...

Ngày tải lên: 31/03/2014, 07:20

... adaptation of RCC cells to the lack of calbindin protective function. The protection from apoptosis despite the lack of cal- bindin could be explained by the decrease in TRPC4, or by retention of the ... expansion. Re-expression of such inhibitors in angiogenic tumors impedes their progression: these include angiostatin, endostatin, tumstatin, PEDF, SPARC (secreted pro...

Ngày tải lên: 07/03/2014, 05:20

... (Figs 1 and 3). According to the observation made by Lu and Wolfe [23], who used a combination strategy of 4,6-diamino-2-phenylindole (DAPI) staining for the detection of DNA and Azo dye staining ... becomes acidic in the final step of PND [22,23]. DePsipher staining of the macronucleus appeared initially during the stage of autophagosome formation, and persisted...

Ngày tải lên: 20/02/2014, 03:20

... noteworthy in view of their coordinative potential, CeMT1 includes four histidines, whereas CeMT2 only has a terminal one. In the absence of a comprehensive analysis of the metal-binding abilities of ... coordination of the Zn(II) ion bound at the C-terminal moiety of CeMT1 is con- tributed to by histidines, and the number of these involved in metal binding is...

Ngày tải lên: 07/03/2014, 02:20

... Italy Introduction Towards the end of the 1970s, in the beginning of pre-mRNA splicing research [1,2], defining exons and introns was essentially based on observing the final composition of the mature ... splic- ing pattern by steric hindrance of the recruitment of the splicing factors to the targeted splicing competent cis-elements, thus forcing the machinery to...

Ngày tải lên: 06/03/2014, 09:22

... expected following the downregulation of PCNA, coding for a protein involved in the control of DNA replication and CDK2-cyclin A activity [15]. In most cases (about 70%), and as expected, DF-induced ... differences in exposure times during western blot- ting. We also observed a slight increase in SREBF1 protein level triggered in the presence of the NT siRNA, in...

Ngày tải lên: 07/03/2014, 05:20