Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
... catalyzes the cleavage of spermine, resulting in back- conversion to spermidine, and the generation of 3-aminopropanal and H 2 O 2 . Cellular localization of human spermine oxidase T. Murray-Stewart ... providing a link between in ammation and carcinogenesis. Results SMO5 is expressed in human lung cell lines To determine whether the SMO5 splice variant of the s...
Ngày tải lên: 07/03/2014, 05:20
... autolysis of mGFP–cal- pain 7–Strep by GST–IST1 and GST–MIM in vitro. Fig. S2. Intermolecular proteolysis of mGFP–cal- pain 7 C290S –Strep and mGFP–calpain 7 DMIT C290S – Strep by Strep–calpain 7 in ... recombinant proteins, no degraded bands were Fig. 7. Enhancement of autolysis of mGFP–calpain 7–Strep by GST–MIM in vitro. (A) Purification of mGFP–calpain 7–Strep and...
Ngày tải lên: 18/02/2014, 04:20
... quantitation of microgram quantities of protein uti- lizing the principle of protein-dye binding. Anal Bio- chem 72, 24 8–2 54. 33 Glantz SA (1988) Primer of Biostatistics. McGraw-Hill Inc., NY. Proline-rich ... Sequential activation of butyrylcholinesterase in rostral half somites and acetylcholinesterase in motoneurones and myotomes preceding growth of motor axons. Dev...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... group of thymidine, forming dTMP. Human TK1 is cytosolic and its activity is absent in resting cells, appears in late G 1 , increases in S phase coinciding with the increase in DNA synthesis, and disappears ... structure–function rela- tion of cytosolic human thymidine kinase. Kinetic prop- erties and oligomerization pattern of nine substitution mutants of V106. Eur...
Ngày tải lên: 18/02/2014, 12:20
Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx
... staining of 293-VnR and ATDC5 cells expressing the Y373C mutant with anti- FGFR3 and anti-phosphotyrosine sera showed both intracellular and cell-surface phosphotyrosine staining (Figs 2Eb,c and ... 70Z-Cbl (a mutant lacking 17 amino acids in the linker and RING finger domain of c-Cbl) and wild-type or mutant FGFR3 cDNAs as indicated, then immunoprecipitated and blotted as...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... RNA- binding activity of the human protein and also of the murine homologue was investigated further, its biologi- cal function, however, remained unclear [16,17]. The three-dimensional structure of ... (c.719C>T) in exon 7 and an inser- tion mutation c.61 3–6 14insA. This insertion causes a frameshift that starts at Met205 and leads to the intro- duction of a stop codo...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... have been interested in the molecular basis of substrate activation in wild-type and mutants of human BuChE [11,15,16], as well as substrate activation in wild-type and mutants of human and Bungarus ... Protein Sci. 6, 130 2–1 307. 32. Main, A.R. (1979) Mode of action of anticholinesterases. Phar- macol. Ther. 6, 57 9–6 28. 33. Fersht, A. (1999) Structure and M...
Ngày tải lên: 20/02/2014, 23:20
Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... dopamine, R- (–) propylnorapomorphine, and raclopride did not affect oligomerization of D 2L and D 2S in Sf9 and HEK293 cells. Human D 2 dopamine receptors can therefore form consti- tutive oligomers in Sf9 cells and in HEK293 ... differentiation, and in ammatory and immune responses. Consequently, many existing therapeutic agents act by either activating or blocking G...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot
... happening.) Lanes 1–5 in Fig. 5A represent the bands obtained by mixing ETNF-6-H (0.1 lm) and hTNF-a (6.6 lm) during the incubation. Lanes 6–1 0 show the controls. As shown in lane 1, some bands were ... (fragment B) and SRTPS (fragment C), which are the sequences of amino acids 2–6 and 5–9 from the N-terminus of hTNF-a. This means that the recombinant hTNF-a contains i...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
... Identifica- tion of native and non-native structure in kinetic fold- ing intermediates of apomyoglobin. J Mol Biol 355, 13 9–1 56. 25 Jamin M & Baldwin RL (1996) Refolding and unfold- ing kinetics of the ... inhibition of Ngb expression increases neuronal injury upon induction of hypoxia, both in vitro and in vivo [1 3–1 6]. Interestingly, it was shown that Ngb is...
Ngày tải lên: 07/03/2014, 02:20