Báo cáo khoa học: Site specificity of yeast histone acetyltransferase B complex in vivo pot
Báo cáo khoa học: Site specificity of yeast histone acetyltransferase B complex in vivo pot
... containing Hat1 as a catalyticsubunit have been described in yeast < /b> [20,24,33],although the presence of < /b> histones in all of < /b> them hasnot been analyzed. In any case, the absence of < /b> his-tone H3 in ... domains of < /b> histone < /b> H3 and histone < /b> H4. In yeast,< /b> acetylation of < /b> histone < /b> H3 Lys56 and histone < /b> H4 Lys91has been described, and both seem to be linked tonucleosome assembly [35,36]. As shown in ... acetylated histone < /b> H4, to a distinctpredeposition complex. In any case, the presence of< /b> the HAT -B complex lacking histone < /b> H3 in the solublecell fraction would indicate that the interaction of< /b> histone...
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Tài liệu Báo cáo khoa học: Substrate specificity of the pseudouridine synthase RluD in Escherichia coli doc
... rRNA-mod-ifying enzymes, including RluD.RluD belongs to the RluA family [2]. Binding of< /b> RluA to one of < /b> its substrates, tRNAPheanticodonstem-loop, induces reorganization of < /b> the RNA [21]. Anability of < /b> ... out of < /b> eight uri-dine residues in 16S rRNA to pseudouridine [9].We have analyzed the substrate specificity < /b> of < /b> thepseudouridine synthase RluD in vivo by using 23SrRNA variants. Single point ... lLofCMCT ⁄ BEU buffer (1 m CMCT in BEU buffer) (CMCT;Sigma-Aldrich Chemie GmbH, Steinheim, Germany) wereadded. One hundred microlitres of < /b> BEU buffer were addedto 15 lg of < /b> rRNA in 20 lL of...
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Báo cáo khoa học: Substrate specificity of vaccinia virus thymidylate kinase docx
... of < /b> ADP favorablyincreased the affinity of < /b> dTDP and dTMP, by a factor of < /b> two as also observed in MABA-dTDP binding.The conformation of < /b> the complex kinase-ADP is thenmore favorable to dTDP binding. ... speci-fic probe for the NMP binding site < /b> of < /b> UMP-CMPkinase. Protein Sci 8, 2697–2704.26 Bone R, Cheng YC & Wolfenden R (1986) Inhibition of < /b> adenosine and thymidylate kinases by bisubstratesanalogs. ... preventcomplete closing of < /b> the LID domain on top of < /b> bothsubstrates. The exact binding site < /b> for the MABA moi-ety is not known. In contrast with the results reportedby Rudolph et al. on MABA-CDP binding to...
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Báo cáo khoa học: Aglycone specificity of Escherichia coli a-xylosidase investigated by transxylosylation ppt
... inhibi-tors of < /b> intestinal a-glucosidases.ResultsAcceptable substrate at the aglycone-binding site< /b> To determine the acceptor specificity < /b> for subsite +1 of< /b> YicI (for nomenclature of < /b> sugar-binding subsites, ... cleaves its linkage [4];however, the aglycone specificity < /b> of < /b> this enzyme hasremained obscure. In this study, we characterized theaglycone-binding site < /b> of < /b> YicI by determining acceptor specificity < /b> ... weretested as inhibitors of < /b> intestinal a-glucosidases. Inhibi-tors of < /b> intestinal a-glucosidases have a therapeutic use in diabetes, which causes higher blood sugar concen-trations than in the normal...
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Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc
... 33 in the UGT 2B4 isoform was replaced by tyrosine in UGT 2B7 , the mutation of < /b> this residue into leucine in UGT 2B7 was also performed. We also mutated thephenylalanine 33 residue of < /b> UGT 2B4 into ... counterpart in UGT 2B1 3 did not change the specificity < /b> of < /b> this isoform.The aim of < /b> this study was to identify amino acid res-idues that are involved in substrate specificity < /b> of< /b> UGTs 2B4 and 2B7 in order ... of < /b> UGT 2B7 in the binding of < /b> morphine. When this charged aminoacid was substituted with alanine, a dramatic decrease in activity was observed. In agreement, the structure–function analysis of...
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Báo cáo khoa học: The specificity of alcohol dehydrogenase with cis-retinoids Activity with 11-cis-retinol and localization in retina docx
... CRALBP, cellular ret-inaldehyde binding protein; CRBP, cellular retinol binding protein;DAB, 3,3¢-diaminobenzidine tetrahydrochloride; RPE, retinal pig-ment epithelium; SDR, short-chain dehydrogenase/reductase.(Received ... technique.Protein concentration was determined by a dye bindingassay (Bio-Rad) using bovine serum albumin as standard[30].Enzyme kineticsStandard ADH activity was determined by measuringthe change in ... ADH4 band was observed in a Western blot of < /b> eye homogenate [12]. The boundprimary antibody was visualized by the Vectastain EliteABC kit (Vector Laboratories, Inc.), using biotinylatedantirabbit...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... againstblackleg and black spot fungal infections.AbbreviationsBGT, brassinin glucosyl transferase; BH, brassinin hydrolase; BHAb, brassinin hydrolase from A. brassicicola; BHLmL2, brassinin hydrolasefrom ... ForNHSNSCH3CyclobrassininA B CFig. 8. Lineweaver–Burk plots of < /b> BH activities for (A) BHLmL2 and (B) BHAb in the presence of < /b> the phytoalexin cyclobrassinin.Enzyme activity was determined as described in ... for BHLmL2 and (B) brassinin saturation curve for BHAb. Themixture was incubated at 23 °C for 45 min in the presence of< /b> increasing concentrations of < /b> brassinin (0–1 mM). The curvesobtained...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... differentorientation of < /b> the substrate w ithin the substrate binding site < /b> of < /b> PAT2, and this may simultaneously avoid theinterference of < /b> the polar side ch ain w ith c orrespondingamino acid residues in the binding ... Eadie–Hofstee toa s ingle k inetic term excluding the possibility t hat k ine ticparameters are s ubstantially affected by unspecific protonbinding effects or via an allosteric proton binding site.< /b> StatisticsAll ... ordered binding mechan-ism where the proton b inds before the substrate enters thebinding domain. Proton transfer to the internal membraneside however, requires t he substrate a s no i ntrinsic...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... proteins onlicensed chromatin in the G1phase was detached fromchromatin. Phosphorylation of < /b> MCM4 and other fac-tors may be involved in stronger binding of < /b> MCM4protein to chromatin in the ... presence of < /b> geminin, which inhibits the ability of < /b> CDT1 to load MCM proteins onto chromatin, mayhelp prevent re-replication of < /b> DNA in the G2and Mphase. During interphase, chromatin-bound MCM4 ... re-replication of< /b> DNA in a single cell cycle. Inactivation of < /b> CDK1 leadsto re-replication of < /b> DNA in eukaryotic cells includinghuman cells [7]. Targets of < /b> the kinase in the regulation of < /b> DNA replication include...
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Báo cáo khoa học: Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase pot
... expressed as nmol of < /b> a lesion per mg of < /b> DNA. Theconcentration of < /b> each lesion in the incubation buffer wascalculated on the basis of < /b> the DNA concentration (50 lgper 50 lL of < /b> incubation buffer). The ... FEBS 5155tural data [42], the DNA-binding interface of < /b> theprotein could be affected.OGG1-A288V has been observed in patients withAlzheimer’s disease [29]. A very limited kinetic analysis of < /b> ... apurinic ⁄ apyrimidinic (AP) lyaseactivity that, after base excision, cleaves the DNAbackbone by elimination of < /b> the 3¢-phosphate of < /b> thedamaged deoxynucleotide (b- elimination) [11,16,17].Owing...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP