Báo cáo khoa học: Site specificity of yeast histone acetyltransferase B complex in vivo pot

... containing Hat1 as a catalytic subunit have been described in yeast < /b> [20,24,33], although the presence of < /b> histones in all of < /b> them has not been analyzed. In any case, the absence of < /b> his- tone H3 in ... domains of < /b> histone < /b> H3 and histone < /b> H4. In yeast,< /b> acetylation of < /b> histone < /b> H3 Lys56 and histone < /b> H4 Lys91 has bee...

Ngày tải lên: 07/03/2014, 05:20

... rRNA-mod- ifying enzymes, including RluD. RluD belongs to the RluA family [2]. Binding of < /b> RluA to one of < /b> its substrates, tRNA Phe anticodon stem-loop, induces reorganization of < /b> the RNA [21]. An ability of < /b> ... out of < /b> eight uri- dine residues in 16S rRNA to pseudouridine [9]. We have analyzed the substrate specificity < /b> of < /b> the pseudouridine syntha...

Ngày tải lên: 18/02/2014, 16:20

... of < /b> ADP favorably increased the affinity of < /b> dTDP and dTMP, by a factor of < /b> two as also observed in MABA-dTDP binding. The conformation of < /b> the complex kinase-ADP is then more favorable to dTDP binding. ... speci- fic probe for the NMP binding site < /b> of < /b> UMP-CMP kinase. Protein Sci 8, 2697–2704. 26 Bone R, Cheng YC & Wolfenden R (1986) Inhibition of...

Ngày tải lên: 16/03/2014, 14:20

... inhibi- tors of < /b> intestinal a-glucosidases. Results Acceptable substrate at the aglycone-binding site < /b> To determine the acceptor specificity < /b> for subsite +1 of < /b> YicI (for nomenclature of < /b> sugar-binding subsites, ... cleaves its linkage [4]; however, the aglycone specificity < /b> of < /b> this enzyme has remained obscure. In this study, we characterized the agl...

Ngày tải lên: 23/03/2014, 07:20

... 33 in the UGT 2B4 isoform was replaced by tyrosine in UGT 2B7 , the mutation of < /b> this residue into leucine in UGT 2B7 was also performed. We also mutated the phenylalanine 33 residue of < /b> UGT 2B4 into ... counterpart in UGT 2B1 3 did not change the specificity < /b> of < /b> this isoform. The aim of < /b> this study was to identify amino acid res- idues that are involved...

Ngày tải lên: 23/03/2014, 09:21

... CRALBP, cellular ret- inaldehyde binding protein; CRBP, cellular retinol binding protein; DAB, 3,3¢-diaminobenzidine tetrahydrochloride; RPE, retinal pig- ment epithelium; SDR, short-chain dehydrogenase/reductase. (Received ... technique. Protein concentration was determined by a dye binding assay (Bio-Rad) using bovine serum albumin as standard [30]. Enzyme kinetics Standard ADH activity was...

Ngày tải lên: 23/03/2014, 12:20

... against blackleg and black spot fungal infections. Abbreviations BGT, brassinin glucosyl transferase; BH, brassinin hydrolase; BHAb, brassinin hydrolase from A. brassicicola; BHLmL2, brassinin hydrolase from ... For N H S N SCH 3 Cyclobrassinin A B C Fig. 8. Lineweaver–Burk plots of < /b> BH activities for (A) BHLmL2 and (B) BHAb in the presence of < /b> the phytoalexin cyclobrassinin....

Ngày tải lên: 18/02/2014, 14:20

... different orientation of < /b> the substrate w ithin the substrate binding site < /b> of < /b> PAT2, and this may simultaneously avoid the interference of < /b> the polar side ch ain w ith c orresponding amino acid residues in the binding ... Eadie–Hofstee to a s ingle k inetic term excluding the possibility t hat k ine tic parameters are s ubstantially affected by unspecific proton bin...

Ngày tải lên: 07/03/2014, 16:20

... proteins on licensed chromatin in the G 1 phase was detached from chromatin. Phosphorylation of < /b> MCM4 and other fac- tors may be involved in stronger binding of < /b> MCM4 protein to chromatin in the ... presence of < /b> geminin, which inhibits the ability of < /b> CDT1 to load MCM proteins onto chromatin, may help prevent re-replication of < /b> DNA in the G 2 and M phase...

Ngày tải lên: 16/03/2014, 13:20

... expressed as nmol of < /b> a lesion per mg of < /b> DNA. The concentration of < /b> each lesion in the incubation buffer was calculated on the basis of < /b> the DNA concentration (50 lg per 50 lL of < /b> incubation buffer). The ... FEBS 5155 tural data [42], the DNA-binding interface of < /b> the protein could be affected. OGG1-A288V has been observed in patients with Alzheimer’s...

Ngày tải lên: 23/03/2014, 05:22