Báo cáo khoa học: Peroxiredoxins as cellular guardians in Sulfolobus solfataricus – characterization of Bcp1, Bcp3 and Bcp4 pot

... analysis of bcp1, bcp3 and bcp4 under oxidative stress The involvement of bcp1, bcp3 and bcp4 in oxidative stress was investigated by assessing mRNA levels fol- lowing treatment of S. solfataricus ... thermophilicity of recombinant Bcp1, Bcp3 and Bcp4, we investigated the peroxidase activities of the enzymes by measuring H 2 O 2 removal at increasing tempe...

Ngày tải lên: 07/03/2014, 05:20

... [6]. A complex consisting of the interacting domains of yeast Hsp90 and human Cdc37 has been crystallised and its structure deter- mined [16]. Amino acids 16 4–1 70 and 20 4–2 08 of human Cdc37 were ... (p50); an N-terminal domain consisting of amino acids 1–1 26, a middle region com- posed of residues 12 8–2 82 and a C-terminal domain of amino acids 28 3–3 78 [8...

Ngày tải lên: 16/03/2014, 22:20

... sig- naling for hypoxia was of interest in this study because QDs, which are redox-active NPs, can release Cd 2+ and induce the intracellular formation of ROS, and, as such, make good candidates ... resulting in an increase in glucose uptake and an up-regulation of glycolysis [37,38]. In addition, PI3K signaling has been shown to be involved in the up-regulation of li...

Ngày tải lên: 07/03/2014, 00:20

... containing 0.2 M ethanolamine and 5 mM 2-mercaptoethanol, in a volume of 1.0 mL. Spectra were measured at 5 min of incubation (solid lines). Spectra of the denaturated and neutralized (dotted lines) ... cobalamin. The regions shown by oblique lines and shadows indicate the adenine-binding pocket and the cobalamin- binding site of enzyme, respectively. Coenzymic functions of...

Ngày tải lên: 07/03/2014, 21:20

... ligand-binding domain; a transmembrane domain; an intracellular tyrosine kinase domain; and a C-terminal regulatory domain [2]. The extracellular domain is subdivided further into four domains. ... ligand binding, as in the case of EGFR vI. EGFR vIII is reported to occur in 3 0–5 0% of glioblastomas [6]. In lung cancers, EGFR vIII is found in 5% of squamous cell carcinomas...

Ngày tải lên: 16/02/2014, 09:20

... our experimental setup. Instead of dividing datasets during 10-fold cross-validation into training and test subsets with the ratio of 9:1 we randomly split the data into training, validation and test sets ... increasing experience In our first experiment we applied 10-fold cross- validation on datasets ranging from 500 to 2500 words with the goal of measuring how the learning impro...

Ngày tải lên: 20/02/2014, 04:20

... effect of bending strain There are several indications of a redistribution of the strains exerted on the oligonucleotide by the bend: partial unstacking of some adenine, thymine and guanine bases and ... cm )1 (dT) in response to base destacking in the oligonucleotides [29,30,4 1–4 3,4 5–4 9]. Between 10 and 25 °C, within the premelting domain, spectral changes are reflec...

Ngày tải lên: 07/03/2014, 09:20

... higher than that of the unactivated 2C zymogen, and the difference is again the result of both a decrease in k cat and an increase in K m . The increase in k cat on activation of the 1C and 2C zymogens ... stability of the 1C zymogen increases on activation, perhaps as the result of the release of strain. Affinity for ribonuclease inhibitor and cytotoxicity RI recog...

Ngày tải lên: 07/03/2014, 11:20

... with the cinnamate. In conclusion, a combination of homology modelling and site-directed mutagenesis of CYP73A1 has identified N302 and I371 as key determinants of substrate binding and orientation ... mutagenesis of the involve- ment of some of these residues in the docking and catalysis of cinnamic acid. The impact of the active site mutations on the binding an...

Ngày tải lên: 17/03/2014, 10:20

... of the serine protease inhibitor family, and is involved in the in ammatory response. It is mainly synthesized in the liver [14] and is a posi- tive acute-phase reactant. During the acute phase response, ... is composed of three typical functional domains: an N-terminal region (transactivation function 1, AF-1), a DNA-binding domain, and a ligand-binding domain. Nur77 has no sp...

Ngày tải lên: 23/03/2014, 07:20