Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

... activity of v-KIND induces the phosphoryla- tion of MAP2 by JNK1 and ⁄ or ERK via the activation of the Ras–Raf–MAP kinase pathway [12]. These results appear to be in agreement with those of previous ... region. The C-lobe of protein kinases mediates the interaction with activators, substrates and regulatory subunits, implying that the KIND domain, an atypical nonc...

Ngày tải lên: 14/02/2014, 19:20

... subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis Satomi Ishii* , †, Akiyo Koshiyama*, Fumika N. Hamada, Takayuki Y. Nara, Kazuki Iwabata, Kengo Sakaguchi ... cloning of CcCac1L and its interaction with CcLim15. (A) Schematic diagram of the CAF-1 large subunits in human, C. cine- rea and S. cerevisiae....

Ngày tải lên: 07/03/2014, 05:20

... than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP. On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, ... molecules, and the mixture was used as an analyte in SPR analysis. ATP and 2-oxoglutarate are known to be the primary effec- tors involved in PII signaling, and they st...

Ngày tải lên: 06/03/2014, 00:21

... and BSA as standard. Rabbit poly- clonal antibodies for TIPRL (A3 0 0–6 6 3A) , PP2Ac (A3 0 0– 73 2A) and a4 (A3 0 0–4 7 1A) were used in immunoprecipita- tion assays, and antibody for murine MafB (A3 0 0–6 1 2A) , which ... independent of the canonical A and B regulatory subunits. Type 2A phosphatases are key players in the yeast target of rapamycin (TOR) signali...

Ngày tải lên: 07/03/2014, 05:20

... in the appearance of an additional peak at 7.65 mL on the elution profile. Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and ... were scanned and evaluated by the ONEDSCAN program. The intensity of the band of unhydrolyzed actin was plotted against the time of incubation. Characterization...

Ngày tải lên: 20/02/2014, 23:20

... [ibuprofen], respectively, and k þ off is the k off value obtained in the absence of ibuprofen. Data analysis Kinetic and thermodynamic data were analyzed with the matlab program (The Math Works, Natick, MA, USA). The ... FA1 and the ligand-binding pockets FA2 and FA6, all located in domains I and II of (t)HSA. Abbreviations FA, fatty acid; HSA, human serum albumin; H...

Ngày tải lên: 06/03/2014, 01:20

... this, the exposed thiol will preferentially sustain the oxidative damage, rather than another amino acid, as a result of its greater reactivity with most damaging species, thereby acting as a local ... on the surface of proteins are the dominant intracellular thiol and that they may play an important role in intracellular antioxidant defences. Results Quantification of expo...

Ngày tải lên: 06/03/2014, 09:22

... helices, a1 a4 (Fig. 2B). Strand b3 has a substantial twist at residues 16 8–1 69. The longest a- helix (a1 ) starts at the end of the GGQ loop and has a bend at residues 19 5–1 96. There are also several loops ... graphical comparison of the experimentally measured parameters against simulated datasets (Fig. 4). Data indicated by gray squares were simulated using the...

Ngày tải lên: 07/03/2014, 05:20

... hoang ba't ddng san d Hoa Ky. Bay gid ngUdi ta cho rang nguyen nhan cua ta't ca cac KH nay la sii dau cd cua cac ngan hang va cdng ty da qud'c gia de bu vao cai da ma't ... cac nfldc dang phat trien (A. Panagaraya, 2005): 1. Cac nUdc da phat trien bao ve bien gidi va trd cap rat cao. 2. Trd ca'p va bao ve ciia cac nUdc da phat trien ra't cd b...

Ngày tải lên: 17/02/2014, 05:20

... holoenzyme/apoenzyme (Table 1), the stability of the protein was affected by each of the mutations Table 4. Apparent a nity of DDT and testosterone for CYP 6A2 wt and CYP 6A2 vSVL. For each apparent a nity calculated, ... in all lanes loaded with bacterial protein. The CYP 6A2 variants have the same apparent molecularmassasCYP 6A2 fromD. melanogaster microsomes. The ap...

Ngày tải lên: 19/02/2014, 12:20