Báo cáo khoa học: Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity docx

... in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity Gernot Rauch 1 , Heidemarie Ehammer 1 , Stephen Bornemann 2 and ... Neurospora crassa chorismate synthase with alanine, producing two single-mutant proteins (Ser16Ala and Ser127Ala) and a double-mutant protein (Ser...

Ngày tải lên: 07/03/2014, 05:20

... Several DSPs have been established as MAPK phosphatases that dephosphorylate the Tyr and Thr residues in the activation loop of MAPKs and thereby attenuate signaling [17,19]. In addition, there is a ... JSP1 was myristoylated at its N-terminus. (A) Schematic representation of JSP1. The potential myristoylation site (Gly2) is indicated by an asterisk. Amino acids critical...

Ngày tải lên: 16/02/2014, 14:20

... generation. The resulting serum was immunoaffinity purified and tested for reaction against HydA1 and HydA2 overexpressed in E. coli and against HydA2 in anaerobically induced algal extracts. Algal ... by PCR in 50 lL reactions that contained 1 lLofKOD HOTSTART polymerase (Novagen, Madison, WI, USA), 25 lL of BAC clone cell-free lysate as template, 125 ng each of forward (5¢-...

Ngày tải lên: 20/02/2014, 11:20

... strain FD6. This promoter was generated by hybridization of two complementary oli- gonucleotides, Part-1 (5¢-AGCTTGACAGGCCGGAATAT TTCCCTATAATGCGCTGCA-3¢) and Part-2 (5¢-GCG CATTATAGGGAAATATTCCGGCCTGTCA-3¢), ... production in the absence of CrtIb, and instead it leads to the accumulation of the intermediates phytofluene and f-carotene in decreasing amounts. On the other hand,...

Ngày tải lên: 16/03/2014, 05:20

... species and transgenic plant lines. Acyl composition in seeds from C. alpina, C. palaestina and wild type and fad2 mutants of nontransforme d A. thaliana and transformed with either the C. alpina D12 ... 12(E )and1 2(Z) isomers of 18:2. The catalytic activity of the C. alpina acetylen ase enzyme is compared with that of the evolutionarily closely related Crepis pala...

Ngày tải lên: 16/03/2014, 18:20

... Edmonton, Canada Human apolipoprotein E (apoE) is comprised of two structural domains, a 22 kDa N-terminal (NT) domain and a 10 kDa C-terminal (CT) domain [1,2]. Studies conducted with isolated domains ... increased lipid binding activity of HT apoE3-NT is maintained in the context of full-length apoE. It is conceivable that an NT domain with increased lipid binding...

Ngày tải lên: 30/03/2014, 11:20

... compared against database assemblies using BLASTN and BLASTX . Screening of a phage genomic library and isolation of full-length gene The spiny do gfish phage-DNA library made in kGEM-11 (Promega, ... in plasma membrane of Chinese hamster ovary and HEK293 cells but the S acMC5R was retained in intracellular compart- ments of these cells hindering pharmacological character...

Ngày tải lên: 30/03/2014, 15:20

... the casein kinase 2 pro- tein. Phosphorylation assay on the S13 3A ⁄ S13 4A mutant form of recombinant, His-tagged Grx4 (see Supporting information) was performed in the same way, but with a lower ... VII and XIV of Saccharomyces cerevisiae and basic phenotypic analysis of the mutant strains. Yeast 16, 255–265. 39 Marcand S, Gilson E & Shore D (1997) A protein- counting...

Ngày tải lên: 07/03/2014, 04:20

... PP2C enzymes may also have additional domains, for exam- ple, Arabidopsis thaliana ABI1 in which the catalytic domain is fused to an EF-hand motif, and human STP (HsSTP), which has an additional 8 kDa a- helical domain ... homolog- ous residues to Asp118 and Asp191 in HsSTP (Asp146 and Asp239) and, of course, in SaSTP (Asp118 and Asp192). Our SaSTP crystal structure...

Ngày tải lên: 07/03/2014, 09:20

... 2008) doi:10.1111/j.1742-4658.2008.06564.x Coronavirus nucleocapsid protein is abundant in infected cells and partici- pates in viral RNA replication and transcription. The central domain of the nucleocapsid protein contains several arginine ... reports have suggested that the SARS- CoV N protein can act as a substrate of various kinases, such as cyclin-dependent kinases, gl...

Ngày tải lên: 23/03/2014, 07:20