... Phytoene synthase genes in tomato (Solanum lycopersicum L. ) – new data on the structures, the deduced amino acid sequences and the expression patterns Giovanni Giorio, Adriana Lucia Stigliani ... and with a pulpy interior rich in seeds [2]. Among the 12 wild relatives of tomato, there is only one (Solanum pimpinellifolium B. Jus...
Ngày tải lên: 07/03/2014, 05:20
... no-template control (negative control) was also included in each assay. Fig. 6. Plasma cortisol levels in the three Angora genotypes (n = 10 per group) following intravenous insulin injection. Plasma glucose ... three groups, and each group demonstrated a decrease in plasma glucose and an increase in plasma cortisol levels in response to insulin injection (Fig. 6). However,...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khóa học: Hydrolytic cleavage by a group I intron ribozyme is dependent on RNA structures not important for splicing pot
... hydrolysis, and full-length intron RNA circle formation. We recently reported that DiGIR2 splicing (intron excision and exon ligation) competes with hydrolysis and subsequent full-length intron ... 3¢-SS is initiated when the last intron nucleotide (TG) binds to the GBS prior to exoG. Splicing and hydrolysis are competing reactions leading to ligated exons and full-length intron...
Ngày tải lên: 23/03/2014, 12:20
Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf
... Switzerland). Two-dimensional crystallization of the c ring For crystallization in 2D according to the method described previously [22], a sample of c ring (2 mgÆmL )1 ) was mixed with 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (Avanti ... critical and long-standing question in (bacterial) bio- energetics is whether the ratio of translocated ions to ATP for a given ATP...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: A point mutation in the ATP synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction pptx
... suspension into the luminometer cuvette containing the b asic solution and the luciferine/luciferase detection kit (plus additional 80 · 10 3 light units ÆmL )1 of luciferase). This method has already ... acidified chromatophores suspension into a l uminometer cuvette containing a basic solution and monitoring the ATP concentration c ontinuously with luciferine/luciferase i n...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Large conformational changes in the Escherichia coli tryptophan synthase b2 subunit upon pyridoxal 5¢-phosphate binding pot
... model included residues 1 7–2 58, 30 9–3 97, three sulfate ions and 23 water molecules. The holo-Ecb model included residues 3–2 90, 29 3–3 97, one PLP molecule, two sulfate ions, one glycerol molecule ... from the peak 3 region. The C terminus of the long-loop and the N terminus of Helix 10 are pulled into the interior of the molecule by hydrogen bonds between the...
Ngày tải lên: 15/03/2014, 11:20
Báo cáo khoa học: Glycogen synthase kinase 3b and b-catenin pathway is involved in toll-like receptor 4-mediated NADPH oxidase 1 expression in macrophages ppt
... ++LiCl Nox1 -actin B Con MergeFITCDAPI LPS LiCl LPS LiCl A –+ –+ LPS – ++LiCl -catenin -actin D -catenin EGFP- GSK3 GSK3 –+ –+ LPS –+ –+ LPS Vec GSK3 S9A F -actin Nox-1 Vec GSK3 S9A DAPI TRITC Merge - LPS - LPS Vector GSK3 S9A E Fig. ... which was also translocated more into the nucleus, than cells stimulated with LPS alone (Fig. 4A,B). Also, Nox1 expression, using LPS p...
Ngày tải lên: 22/03/2014, 21:21
Báo cáo khoa học: Engineering triterpene production in Saccharomyces cerevisiae – b-amyrin synthase from Artemisia annua docx
... min at 17 900 g to pellet cells. The cells were resuspended in 0.6 mL of a fresh solution of 20% (w ⁄ v) KOH in 50% ethanol containing 30 lgÆmL )1 cholesterol as an internal standard. The cells ... perspective. The effect of plant saponins on low-density lipoprotrein cholesterol absorption and arterial atherosclerosis has received much attention, leading to the development...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: "Veterinary decision making in relation to metritis - a qualitative approach to understand the background for variation and bias in veterinary medical records"
... (Figure 2). Three levels of decision were revealed: cow level (individ- ual cows), farm level (multiple cows in a specific farm) and population level (multiple cows in multiple farms). None of the ... decisions and included population level considerations and general evidence into the criteria (e.g. using the same cut-off value on metritis scale in all herds), they were...
Ngày tải lên: 25/10/2012, 10:45
Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc
... Mu ¨ ller glia cells of the cone-dominant chicken retina to provide additional 11cRAL for cones [2 4–2 7]. The Mu ¨ ller cell is the prin- cipal glial cell type in the vertebrate retina, comprising a ... negative cells are indicated by yellow arrows. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer. ONL, outer nuclear layer; OPL, outer plexiform layer...
Ngày tải lên: 14/02/2014, 14:20