Báo cáo khoa học: Changes in specific lipids regulate BAX-induced mitochondrial permeability transition pptx

... effect on BAX-induced mPT E. Martı ´ nez-Abundis et al. 6510 FEBS Journal 274 (2007) 6500–6510 ª 2007 The Authors Journal compilation ª 2007 FEBS Changes in specific lipids regulate BAX-induced mitochondrial ... this study was to investigate the relevance of the main components of membrane microdomains (e.g. cholesterol and sphingolipids) in activation of the mitochondrial...

Ngày tải lên: 07/03/2014, 05:20

... Max-Planck-Institute of Biochemistry, Martinsried, Germany The family of activating enhancer-binding protein (AP)-2 transcription factors regulate their target genes through binding to the palindromic ... analyzed by PCR using specific primer pairs generating frag- ments spanning the first (Sox9_1) or the second (Sox9_2) Sox9-binding site of the AP-2e promoter. Sox9 binding to both binding...

Ngày tải lên: 18/02/2014, 08:20

... major interaction site for the adenine moiety. In b1, an arginine residue (Ile417 in rPDE2) binds to N1 and a threonine (Ser419 in rPDE2) inter- acts with N7 and the N 6 amino group. The inter- actions ... cGMP binding [13], and, in PDE6, both of the amino acids corresponding to Phe433 ⁄ Asp434 in rPDE2A are required for cGMP binding [14]. Our initial mutations in cyaB1 GAF B also...

Ngày tải lên: 19/02/2014, 02:20

... Protein alteration in mitochondrial matrix with aging (Eur. J. Biochem. 270) 2297 ADP/O ratios obtained with succinate or glutamate indicate no change in coupling efficiency. The yield of mitochondrial preparation ... been found in several mammals including human tissues and cell lines [24–26]. It is responsible for the degradation of abnormal proteins and certain short-lived specifi...

Ngày tải lên: 20/02/2014, 11:20

... 10–15 min, and then in ATP staining buffer (3.6 mm ATP, 20 mm barbital, 18 mm CaCl 2 , pH 9.4) for 2 h. Incubation in 1% CaCl 2 for 10 min was followed by incubation in 2% CoCl 2 for 10 min and in ... tropo- nin I slow . Troponin I fast , however, is not changed in a reciprocal manner, indicating that troponin I slow and troponin I fast respond independently to distinct fast and slo...

Ngày tải lên: 07/03/2014, 05:20

... mem- brane binding affinity enhancement by minor increases in the overall on-rate and ⁄ or minor decreases in the overall off-rate. The results obtained in the present study support that the binding of ... randomized. No binding was detected in buffer lacking Ca 2+ for any of the proteins tested. Protein binding equilibrium data (R eq ) were fitted to a one- site binding hyperbola accor...

Ngày tải lên: 18/02/2014, 06:20

... leading to in utero death at E15 [2,3]. The c-Myb protein consists of an N-terminal DNA-binding domain (DBD), a central trans- activation domain (TAD) and a C-terminal negative regulatory domain ... seems to increase the fraction of Myb species in the insoluble part after subnuclear fractionation, which indicates that sumoylation might be involved in modulating the protein–protein inter...

Ngày tải lên: 21/02/2014, 00:20

... tyrosinase 320 amino acids Core domain 36–357 Copper binding motif Arg40 Cys84 Tyr349 Tyr347 Copper binding motif Trypsinisedpro-tyrosinase 332 amino acids Core domain 36–370 Copper binding ... likely histidine 80 in the V. spinosum tyrosinase. Another motif, which is present not only in tyrosinases, but also in the oxygen transporting haemocyanin proteins, is the PYWDW (amino ac...

Ngày tải lên: 06/03/2014, 11:20

... residues of in its loop domain, including Ser70 and Ser87, is critically involved in the apoptotic process, and is induced by microtubule-damaging agents such as paclitaxel, docet- axel, vincristine ... Martinou JC et al. (1997) Bcl-2 undergoes phosphorylation by c-Jun N-terminal kinase ⁄ stress-acti- vated protein kinases in the presence of the constitu- tively active GTP-binding prote...

Ngày tải lên: 07/03/2014, 05:20

... virions from infected cells by binding to E2 in the ER and recruiting viral particles into exo- somes. When expressed in Chinese hamster ovary (CHO) cells, E1 and E2 were retained in the ER. Co- expression ... [56]. In contrast to promoting infectivity of HCVpp and HCVcc, the role of CD81 in binding and internalizat- ion of clinical HCV isolates is not as clear. Antibodies against C...

Ngày tải lên: 19/02/2014, 06:20