Báo cáo khoa học: Effect of annealing time of an ice crystal on the activity of type III antifreeze protein pdf

... Effect of annealing time of an ice crystal on the activity of type III antifreeze protein Manabu Takamichi1,2, Yoshiyuki Nishimiya1, Ai Miura1and Sakae Tsuda1,21 Functional Protein ... growthinhibition ability of an AFP [5]. Therefore, determina-tion of the Tiniand Tm of a seed ice crystal is an essen-tial procedure to evaluate the TH activity of AFP.However, the mechanism of ice ... we examined the dependence of TH on experimental parameters, including cooling rate, annealing time, annealing temperature and the size of the seed ice crystal for an isoform of type III AFP from...

Báo cáo khoa học: "Parsing with Treebank Grammars: Empirical Bounds, Theoretical Models, and the Structure of the Penn Treebank" ppt

... grammatical saturation, in-cluding analysis of the strongly connectedcomponents of the phrasal nonterminals in the Treebank, and model how, as sentencelength increases, the effective grammar ... NN:[2,3])3.1 Time The parser has an theoretical time bound,where is the number of words in the sentence to beparsed, is the number of nonterminal categories in the grammar and is the number of (active) ... span.Deﬁnition 1 The passive (or active) saturation of agiven span is the number of passive (or active) edgesover that span.In the total time and traversal bound, the effective value of...

Báo cáo khoa học: Human ATP-dependent RNA ⁄ DNA helicase hSuv3p interacts with the cofactor of survivin HBXIP ppt

... analyzed the subcellulardistribution of the truncated mutant protein usinganti-myc monoclonal antibodies visualized with anti-mouse secondary antibodies conjugated with FITC. In the case of ... 17%. Then, after transient expression,wildtype and truncated forms of hSuv3p, the proteinswere visualized by anti-myc monoclonal antibodies andanti-mouse Texas Red conjugated secondary antibod-ies. ... glutamine. The cells were then trans-fected using FuGene6 reagent (Roche, Indianapolis, IN,USA). At 24 h after the transfection staining of the mito-chondria and the immunodetection of the tagged proteinswas...

Báo cáo khoa học: Afﬁnity puriﬁcation-mass spectrometry Powerful tools for the characterization of protein complexes ppt

... severalsmaller spliceosomal complexes. The main activity of the spliceosome is the recognition of splice sites in nuclearpre-mRNA and to catalyze the accurate removal of intronsand ligation of exons to ... to the identiﬁcation of noveldrug targets, the reconstruction of pathways and help tounderstand the mechanism of action and side-effects of therapeutic compounds.Correspondence to B. Kuster, ... diversity on the protein level is much higher. In addition to the diversity on the level of primary protein sequence and the presence of modiﬁcations, complexity is further increasedwhen considering the...

Báo cáo khoa học: " Effect of the medical emergency team on long-term mortality following major surgery"

... someestimation about the force with which the victims were hit andswept away by the wave.Antiinfective therapyOur intial choice of anti-infective therapy was a combination of a potent quinolone combined ... and glycopeptides only, and Stenotropho-monas maltophilia, sensitive to ofloxacin only. Polymicrobialwound contamination also included contamination with fungi(for instance, Candida albicans ... cohort of patients.Methods This study is an observational report on complexwound management, infection and psychoemotional controlassociated with the 2004 Tsunami disaster. The setting was an adult...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... recommendation that further researchshould study the effects of sleep deprivation and workschedule interventions on the performance of upper-levelresidents and other medical staff across a variety of disciplines. ... [2].Letter Effect of intern’s consecutive work hours on safety, medicaleducation and professionalismChristopher P Landrigan, Steven W Lockley and Charles A CzeislerDivision of Sleep Medicine, Boston, ... in the description of the limitations that we would like to point out. Dr Sarani and DrAlarcon note:“There were more patients admitted to the ICU and more ICUpatient-days in the traditional...

Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efﬁcacy pdf

... withpuriﬁed Dicer protein [35]. As expected, the band wasshifted in the presence of the Dicer antibody but not the other antibodies.We also wished to conﬁrm the location of anyTRBP-containing bands, ... location (Fig. S3).Based on molecular weight, both the Dicer and TRBPcomplexes are assumed to contain only one moleculeeach of protein and siRNA. As further conﬁrmation of the identities of the ... the dose-responsive concentration range (Fig. S1). Effect of TRBP or Dicer knockdown on the silencing efﬁcacy of mismatched siRNAsWe hypothesized that the reduction in the function of the mismatched...

Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx

... mm enhanced the binding of InsP6to PhyAsr (Table 1). Given the absence of structural changes as a function of ionic strength andligand binding, the effect of ionic strength on catalytic activity ... H2O2for 45 min and solved the structure of the oxidized protein (PhyAsrox; Protein Data Bank:2PT0). The P-loop in PhyAsroxadopts the openconformation as a result of oxidation of the catalyticcysteine ... test whether the move-ment of the P-loop in PhyAsr is due to oxidation of the catalytic cysteine, we oxidized crystals of PhyAsrTable 1. Effect of ionic strength on the hydrolysis of InsP6byPhyAsr....

Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx

... expression, acetylation of H3 and H4histones, and the remaining DHSs in the deleted cellswere examined to determine the roles of these DHSs on the transcription of Ig-b gene and on the mainten-ance ... of the OCT family transcription factors appeared to beinvolved in the transactivation of the region which had the highest enhancing activity of the three DHSs. The state of acetylation in H3 and ... estab-lishment and maintenance of acetylation in the GHlocus. Because deletion of this region has no effect on the formation of cell type- speciﬁc DHSs, the regionrequired for H3 and H4 acetylation does...

Tài liệu Báo cáo khoa học: Effect of gadolinium on the ryanodine receptor/ sarcoplasmic reticulum calcium release channel of skeletal muscle docx

... gadolinium on the cytoplasmic andalso on the luminal side of the channel. Although insingle channel experiments the luminal Ca2+concen-tration has an effect on the activity of the RyR1[15,16], ... Gd3+.Gd3+inhibited the channel activity from the trans side in aconcentration-dependent manner. (Fig. 3A–C). Decrea-sing the Gd3+concentration on the trans side of the channel by addition of equimolar ... pore [10]. Results about the effect of the luminal (trans) calcium on the activity of the RyR1 arecontroversial depending on experimental conditions.Inhibition of the channel was described at...

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