Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf

... 4865 Colocalization of annexins and dicalcin in frog olfactory and respiratory epithelium Dicalcin has been reported to localize in the cilia of frog olfactory and respiratory epithelium [7]. To understand the ... of dicalcin with annexin A1, annexin A2 or annexin A5 in frog olfactory epithelium. (A–I) Immunofluorescence double- staining of dica...

Ngày tải lên: 07/03/2014, 05:20

... family of around 20 distinct enzymes involved in maintaining the fidelity of protein synthesis through the specific esterification of an amino acid to the 2¢-or 3¢-hydroxyl group of the 3¢-terminal ... min 3.5 4.0 4.5 5.0 5.5 6.0 90 min 63 min 36 min 9 min t / min Fig. 1. (A) Ion exchange HPLC measurement of mononucleotide and dinucleotide levels observed in a LysU catalysis mixt...

Ngày tải lên: 30/03/2014, 10:20

... experimentally the determinant of V H ⁄ V L interaction strength or its relationship with antigen-binding affinity. Previously, the effect of mutations of the V H ⁄ V L interface residues on antigen binding has ... affinity and the V H ⁄ V L interaction strength determined, five clones in the H39Q group showed both high antigen- binding affinity and strong V H ⁄ V L interaction...

Ngày tải lên: 07/03/2014, 12:20

... compared with that of the scaffolding domain, in repeated experiments. Structure of ET B R recognized by caveolin Since caveolin interacts with ET B R via the scaffolding and C-terminal domains, ... suggest that caveolin-1 also utilizes the scaffolding and C-terminal domains to interact with ET B R, as with other signalling molecules. The binding of the C-terminal domain f...

Ngày tải lên: 17/03/2014, 03:20

... solution containing the protein. The binding constant (K b ) was determined using the simplest binding model, assuming that one molecule of ligand binds to one molecule of protein. The molar ... under- stand the molecular basis of the interaction between SlyD and HypB, and this could shed light on the role of the atypical nickel ion-binding PPIase domain of SlyD in the hydro...

Ngày tải lên: 30/03/2014, 01:20

... Nonspecific binding was measured as the binding of 125 I-labelled toxin in the presence of an excess of unlabe- led LT-B. To assay nonspecific binding of 125 I-labelled LT-I or competitive inhibition ... B subunits of toxin were incubated with cells for 30 min at 4 °C and then further incubated with 125 I-labelled LT-I for 60 min at 4 °C. The blocking effect of 125 I-labelle...

Ngày tải lên: 30/03/2014, 10:20

... hydrogen bond with FAD [16], the model structures indicated that it is also involved in interactions with the neigh- bouring monomer. As shown in Fig. 7B, Tyr377 and Asn379 in the loop region interact with ... consists of 10 amino acids, seven of which are conserved within the species (Fig. 2C) and two of which are conserved within all ACAD family of enzymes, forming a...

Ngày tải lên: 18/02/2014, 04:20

... was minimized in two stages. In the first stage, we kept the protein fixed and only minimized the position of the water molecules and ions. In A. Viegas et al. Determinants of ligand specificity in ... for screening and identifying ligand binding to protein receptors. Angewandte Chemie-International Edi- tion 42, 864–890. 16 Mayer M & Meyer B (1999) Characterization of li...

Ngày tải lên: 18/02/2014, 17:20

... in ammatory cytokines, including IL-1b, TNF-a and IFN-c. (B) Chemokines expressed in the joint recruit leukocytes into the joints. In addition to functioning in cell trafficking, several chemokines have ... associated mainly with in ltrating macrophage-like cells and fibroblast-like cells in the RA synovium, and the inter- action of activated leukocytes with FLS results i...

Ngày tải lên: 18/02/2014, 18:20

... that the N-terminal domain is involved in protein–protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspecies interactions are incompatible. From ... [15–18]. In the bovine complex a large subdomain is made up of  20 integral membrane proteins contributing > 60 transmem- brane segments. Some of these must be intimately invo...

Ngày tải lên: 19/02/2014, 16:20