Báo cáo khoa học: Implication for buried polar contacts and ion pairs in hyperthermostable enzymes pot

... 2007 FEBS MINIREVIEW Implication for buried polar contacts and ion pairs in hyperthermostable enzymes Ikuo Matsui and Kazuaki Harata Biological Information Research Center, National Institute ... by distinguishing between the inside polar contacts and the total intramolecular polar contacts, and by evaluating their contribution as molecular determinants for...

Ngày tải lên: 07/03/2014, 05:20

... ferredoxin-GOGAT cycle is the main pathway of inorganic nitrogen assimilation and recycling into glutamine and glutamate, and preconditions amino acid inter- conversion and remobilization. Abbreviations AS, ... 1. Amino acid composition in leaves, and amino acid percentage ratio in the phloem exudates and leaves and xylem bleeding sap and leaves in the light and dark....

Ngày tải lên: 18/02/2014, 18:20

... Myc transformation activity in the rat embryo fibroblast (REF) assay, a surrogate assay for neoplastic transfor- mation [10]. Interestingly, a naturally occurring mouse Mxi1 protein isoform lacking ... observed, includ- ing that Mxi1-SRa appears to be the predominant transcript in the adult intestine and in the developing embryo, whereas Mxi1-SRb transcripts predominate in the adul...

Ngày tải lên: 18/02/2014, 16:20

... including two dominating peaks at approximately m ⁄ z 7000. As mycelia and spores largely remained intact, and the extraction solution contained acetonitrile and methanol, the well-known hydrophobins ... mass peak was missing (Fig. 3B). Deviating post-translational processing of hydrophobins in H. jecorina strains To investigate strain diversity with respect to meta- bolite production...

Ngày tải lên: 19/02/2014, 02:20

... isotonic solution of 4% (v ⁄ v) formaldehyde for 5 min, and washed in running tap water for 5 min. Then, sections were incuba- ted in 85% (v ⁄ v) propyleneglycol for 2 min (the solution was changed ... in iron-rich media showed a further increase in ROS production as deter- mined by an increase in DCFH mean fluorescence intensity (Fig. 2B, thick line). In addition, determin...

Ngày tải lên: 19/02/2014, 16:20

... domain-swapping in the cystatin family [20]. Although both additional cysteines in cystatin F at positions 1 and 37 (cystatin C numbering) can form a disulfide bond [13], the cysteine at position ... regulating papain-like cathepsins involved in antigen presentation. Therefore, interactions of cystatin F with several of its potential targets, cathepsins F, K, V, S, H, X and C, were s...

Ngày tải lên: 19/02/2014, 17:20

... deficient in desmin, again demonstrating inclusion body construc- tion independent of intermediate fialments [62]. Inter- estingly, inclusion body formation is slowed by aB-crystallin, Hsp27 and HspB8, ... opaque with age and contain many inclusion bodies reactive with antibody to aB-crystallin, but not to b- and c-crystallin, suggest- ing an important role for aA-crystallin in main...

Ngày tải lên: 19/02/2014, 18:20

... K & Gearing A (2001) Immunohistochemistry of matrix metalloproteinases in reperfusion injury in rat brain: activation of MMP-9 linked to stromelysin-1 and microglia in cell cultures. Brain Res ... only in microglia ⁄ macrophages, suggesting that fractalkine is involved in neuron– microglia signalling [178]. In fact, this chemokine participates in leukocyte migration and...

Ngày tải lên: 07/03/2014, 03:20

... of tumor induction, administering carcinogen, 3MC, and inoculating with tumor cells. The 3MC compound might produce some secondary effects in the animal in addition to tumor induction. During multiplication inoculated ... concerning the levels of creatine and CK in malignant cells and in tumor-bearing animals. In some recent publications, the ratio of choline to crea- tine has...

Ngày tải lên: 07/03/2014, 06:20

... approach for developing a screening system to identify and charac- terize candidate insecticidal compounds showing both inductive and potentiative activity. Results The DNA-binding domains (DBDs) ... results [25–27], suggest a basis for screening plant extracts and candidate insecticides affecting EcR–USP-mediated induction or potentiation in either or both species. The fold induc...

Ngày tải lên: 18/02/2014, 08:20