Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx

... compilation ª 2007 FEBS Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization Amaury ... C-Terminal D481 C223 A B H464 1 208 560 1102 USP7- FL USP7 1-560 USP7 208-560 USP7 208-1102 EGFP EGFP EGFP EGFP USP7 1-205-EGFP USP7 20-205-EGFP USP7...

Ngày tải lên: 07/03/2014, 05:20

... crotonyl-CoA, 3-hydroxybutyryl-CoA and 3-methylcrotonyl-CoA were used for the kinetic characterization of AUH. The data are summarized in Table 2. Overexpression of a mutant form of AUH and its activity on (E)-3-MG-1-CoA Mutations ... shoulder and the tailing is due to acetyl-CoA and not due to any other compound. (B) The peaks 3, 4, and 5 were isolated by HPLC and used...

Ngày tải lên: 19/02/2014, 07:20

... large number of orthologs (Figs 2,9). This suggests an essential role for FMN despite the low affinity of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. ... homooctamer structure with a relative mass of 309 kDa [29]. In order to check for the potential influence of substrates and cofactors on the quaternary structur...

Ngày tải lên: 19/02/2014, 13:20

... enzyme forms [16,17]. Even though S. cerevisiae is a close rel- ative of Candida species, and is often used as a model pathogen, its DHODH of family 1 is unsuitable as a prototype for the search for ... calculated: pK a1 , 6.7 ± 0.05 for both enzymes; pK a2 , 9.5 ± 0.1 for CaDHODH and 9.9 ± 0.15 for DNCaDHODH. We compared the activity of CaDHODH and DNCaDHODH with a vari...

Ngày tải lên: 07/03/2014, 12:20

... Yusof 1 , Erning Gao 1 , Hong-Li Yan 2 and Andreas Hofmann 1 1 Institute of Structural & Molecular Biology, School of Biological Sciences, The University of Edinburgh, UK 2 Department of ... precipitation assay Calcium-induced precipitation of annexins B1 and A1 was performed as a control for the heparin binding and lipo- some copelleting assays. 10 lLof2mgÆmL )1 prote...

Ngày tải lên: 07/03/2014, 12:20

... domains that are responsible for binding substrate and NADP cofactor. The detailed structural information might expedite the discovery of novel SDH inhibitors and further of antimicrobial agents, though ... expressed, and purified in Escherichia coli system. The enzymatic characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm to...

Ngày tải lên: 19/02/2014, 05:20

... in the 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the function of a diverse number of RNA-binding proteins has been ... that of other well- characterized members of PUF family. These proteins must bind to transcripts in the plant and regulate their translation and ⁄ or turnover. A large number of...

Ngày tải lên: 18/02/2014, 06:20

... (1971) The kinetics and mechanisms of the reactions of iron(III) with b-dik- etones. The formation of monoacetylacetoatoiron(III) and the effect of copper (II) on the formation of monothenoyltrifluoroacetonatoiron ... measurements of con- sumption of O 2 and substrate with the formation of detectable products. Figure S4 shows that conversion of 2,4-pentanedione (5 mm)...

Ngày tải lên: 18/02/2014, 06:20

... at 60 °C for 5 min and then slow cooled to 44 °C before addition of the substrate, which was also at 44 °C. The chase phase was then initiated by removing 5 lL of the reaction mixture and diluting ... lLof8nm 5¢-end labeled sub- strate and allowed to react in a total volume of 6 lL. The ribozyme solution was preincubated at 60 °C for 5 min and then slow cooled to 44 °C befo...

Ngày tải lên: 18/02/2014, 18:20

... Soret band maxima of the azide-free form at 405 nm and of the azide-bound form at 421 nm, so the relative amounts of the nonbound and azide-bound forms were estimated directly from the values of ... with time, indicating the formation of biliverdin. In this case, neither bands of the oxy form (at 541 and 578 nm) nor bands of verdoheme and CO–verdoheme (at  690 and at...

Ngày tải lên: 19/02/2014, 05:20