Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
... compilation ª 2007 FEBS Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization Amaury ... C-TerminalD481C223ABH46412085601102 USP7- FL USP7 1-560 USP7 208-560 USP7 208-1102EGFPEGFPEGFPEGFP USP7 1-205-EGFP USP7 20-205-EGFP USP7 50-205-EGFP USP7 70-205-EGFPFig. 1. Structural functional features and constructs of USP7 designed ... process-ing of ubiquitin and ubiquitin-like proteins. They areresponsible for the disassembly of ubiquitin chains, and for the cleavage of mono- and oligomers of this mole-cule, either in precursor form...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... crotonyl-CoA,3-hydroxybutyryl-CoA and 3-methylcrotonyl-CoAwere used for the kinetic characterization of AUH.The data are summarized in Table 2.Overexpression of a mutant form of AUH and its activity on (E)-3-MG-1-CoAMutations ... shoulder and the tailing is due to acetyl-CoA and not due toany other compound. (B) The peaks 3, 4, and 5 were isolated by HPLC and used as substrates for AUH. The AUH assay contained 2 mM of the ... proceduresProduction and purification of human AUHin E. coliThe production of AUHp40 (precursor form), AUHp32(mature form), AUHp40A240 V and AUHp32A240 V inE. coli was performed using the pMAL-c2...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... large number of orthologs(Figs 2,9). This suggests an essential role for FMN despitethe low affinity of the enzyme for that cofactor and theapparent absence of a redox process as part of the catalyticcycle. ... homooctamerstructure with a relative mass of 309 kDa [29]. In order tocheck for the potential influence of substrates and cofactorson the quaternary structure of the enzyme, we performedboundary sedimentation ... exception of Mycoplasma gallisepticum R, Myco-plasma penetrans and Spiroplasma kunkelii, the genomes of the members of the mollicutes group (total number, 7 out of 10) are devoid of genes for the...
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Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot
... enzymeforms [16,17]. Even though S. cerevisiae is a close rel-ative of Candida species, and is often used as a modelpathogen, its DHODH of family 1 is unsuitable as aprototype for the search for ... calculated:pKa1, 6.7 ± 0.05 for both enzymes; pKa2, 9.5 ± 0.1 for CaDHODH and 9.9 ± 0.15 for DNCaDHODH.We compared the activity of CaDHODH and DNCaDHODH with a variety of native and two artifi-cial ... CaKDHODH-mutrev1 for a firstfragment and with primers CaKDHODH-mutfor1 and CaDHODH-rev for a second fragment. The overlappingPCR fragments were then used as templates for PCR withprimers Ca-forlong2 and...
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Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf
... Yusof1, Erning Gao1, Hong-Li Yan2 and Andreas Hofmann11 Institute of Structural & Molecular Biology, School of Biological Sciences, The University of Edinburgh, UK2 Department of ... precipitationassayCalcium-induced precipitation of annexins B1 and A1 wasperformed as a control for the heparin binding and lipo-some copelleting assays. 10 lLof2mgÆmL)1protein instandard buffer (100 mm NaCl, ... Enzymol372, 186–216.44 Hu N-J, Currid M, Daley M & Hofmann A (2005) Twonew software applications for automated processing of circular dichroism and fluorescence data. Appl Spectrosc59, 68A.45...
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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... domains that are responsible for binding substrate and NADP cofactor. The detailed structural information might expedite the discovery of novelSDH inhibitors and further of antimicrobial agents,though ... expressed, and purified in Escherichia coli system. The enzymatic characterization of HpSDH demonstrates its activity with kcat of 7.7 s)1 and Km of 0.148 mmtoward shikimate, kcat of 7.1 s)1 and ... catalytic proper-ties of HpSDH and the effects of pH and temperatureon HpSDH. The results show that HpSDH has a kcat of 7.7 ± 0.9 s)1, Km of 0.148 ± 0.028 mm and kcat⁄ Km of 5.2 · 104m)1Æs)1toward...
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Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc
... in the 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the function of a diversenumber of RNA-binding proteins has been ... that of other well-characterized members of PUF family. These proteinsmust bind to transcripts in the plant and regulate theirtranslation and ⁄ or turnover.A large number of APUM target candidates: ... 6).Therefore, as has been suggested in yeast and Dro-sophila, APUM proteins may be involved in the regu-lation of many aspects of growth and development.Interaction between APUM proteins and transcripts...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... (1971) The kinetics and mechanisms of the reactions of iron(III) with b-dik-etones. The formation of monoacetylacetoatoiron(III) and the effect of copper (II) on the formation of monothenoyltrifluoroacetonatoiron ... measurements of con-sumption of O2 and substrate with the formation of detectable products. Figure S4 shows that conversion of 2,4-pentanedione (5 mm) proceeded with depletion of a molar equivalent of ... Wellenreuther and W. Meyer-Klaucke for data collection and assis-tance in data evaluation. The assistance of T. Pavkov(Institute of Chemistry, University of Graz) in theacquisition of CD and DLS...
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... at 60 °C for 5 min and then slow cooled to44 °C before addition of the substrate, which was also at44 °C. The chase phase was then initiated by removing5 lL of the reaction mixture and diluting ... lLof8nm 5¢-end labeled sub-strate and allowed to react in a total volume of 6 lL. Theribozyme solution was preincubated at 60 °C for 5 min and then slow cooled to 44 °C before the addition of ... constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic boundto the ribozyme, and [ribozyme]uis the concentration of unbound ribozyme in the reaction.Measurement of rate constant of substrate- cleavage...
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Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
... Soret band maxima of the azide-freeform at 405 nm and of the azide-bound form at421 nm, so the relative amounts of the nonbound and azide-bound forms were estimated directly from thevalues of ... with time, indicating the formation of biliverdin.In this case, neither bands of the oxy form (at 541 and 578 nm) nor bands of verdoheme and CO–verdoheme(at 690 and at 640 nm, respectively) ... for (A) and (B) and 25 K for (C)–(E).(A) The ferric heme complex of GmHO-1 at pH 7.0 (0.1M, KPB).a-1, Expansion of the gxyregion of (A) (solid line) and of the corres-ponding part of the...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ