Báo cáo khoa học: Purification and characterization of zebrafish hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... composed of two each of the a and b subunits. Analysis of the N-terminal transit peptide of CLGAT Analysis of the CLGAT cDNA predicted a chloroplast transit peptide upstream from the N-terminus of CLGAT. ... plants. Images 1 and 2 represent photographs of wild watermelon plants watered daily and subjec- ted to drought for 5 days, respectively, and images 3 and...

Ngày tải lên: 20/02/2014, 03:20

... – – 16 Dog – – 4 Donkey – – 4 Human A – 0 0 Human B – 0 2 Human O – 0 0 Cow – 0 0 Goat – 0 0 Sheep – 0 0 a Data from [30] and [63]. Table 3. The effect of enzyme treatment of rabbit and horse erythrocytes ... surface. They are found at the outermost ends of the sugar chain of animal glycoconjugate. They act as an important compone...

Ngày tải lên: 21/02/2014, 00:20

... (Hdr) is a key enzyme in the energy metabolism of methanogenic archaea. In the final step of methanogenesis, the mixed disulfide of the metha- nogenic thiol coenzymes coenzyme M and coenzyme B is generated ... of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences of the four polypeptides present in the purified...

Ngày tải lên: 21/02/2014, 03:20

... that of medaka hatching enzyme. Finally, the evolution of the mechanism of egg envelope diges- tion is discussed on the basis of the manner of the reciprocal or cross-species egg envelope digestion using ... swells the envelope. The cross-species digestion using enzymes and substrates of zebrafish and medaka revealed that both ZHE1 and MHCE c...

Ngày tải lên: 07/03/2014, 04:20

... cm). The column was run in 20 mm 1,3-diamino propane, pH 11. Ion-exchange chroma- tography of two pools (A and B) that contained protein bands of the expected mass range was then repeated on the Mono ... Biosystems). Acknowledgements Financial support for this research was provided by a grant to T. Kyndt from the ‘Institute for the Promo- tion of Innovation through Science...

Ngày tải lên: 07/03/2014, 11:20

... electrophoresis and N-terminal sequence of the purified enzyme. (A) Native PAGE of the purified enzyme (0.5 lg) in the absence of SDS. (B) SDS ⁄ PAGE of the purified enzyme (2 lg) and N-terminal sequence of bands ... (Cys14–Cys43, Cys2 6– Cys71, Cys62–Cys128, Cys63–Cys67, Cys100–Cys132 and Cys108–Cys119 in human mature cathepsin B). Residues in the S1, S2 and...

Ngày tải lên: 16/03/2014, 04:20

... Department of Microbiology and Immunology and the College of Medicine, by Public Health Service grant CA101931 (to D.J.M.) from the National Institutes of Health, and the Australian Research ... concentration of 0.2 0–0 .25 m M ), 5 0–1 00 pmol of each primer, and 2.5 U of thermostable DNA polymerase. E. coli was transformed by the calcium chloride method. Cons...

Ngày tải lên: 16/03/2014, 16:20

... Cu 2+ and Ba 2+ were strong inhibitors of the analysed enzymes, whereas the presence of monovalent ions in the incubation mixture did not affect the rate of ATP and ADP hydrolysis (Table 4). ATP and ... ATP and ADP and the product of ATP and ADP hydrolysis is AMP – the compound with a very low affinity for P2 receptors. The above results confirm the parti...

Ngày tải lên: 17/03/2014, 10:20

... 5–5 5 min 2 0–4 7% solvent B, and 5 5–8 0 min 4 7–1 00% solvent B. The effluents were separated into two directions, and the flow rate of one direction was adjusted to 0.6 mLÆmin )1 and that of the other ... hemolytic than melittin and more hemolytic than magainin (Fig. 10). Fig. 5. Cation-exchange HPLC of native P1, P2-2 and P3, and their synthetic peptides. Mix...

Ngày tải lên: 23/03/2014, 20:22

... heparan sulfate. The heparin lyases have become increasingly important in understanding the biological roles and structure of the glycoaminoglycans (and proteoglycan), which are involved in the ... involved in the well known anticoagulant activity [7] and the regulation of various cellular processes such as the potentiation of angiogenesis [8] and the modulation...

Ngày tải lên: 23/03/2014, 21:20