Báo cáo khoa học: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily pot
Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... -ATC ATC TCC ATC GAC TAC TCC CTG-3¢, the antisense primer 5¢-AAG AAT TCT AGA TTA ATG GTG ATG ATG GTG ATG ATG GTG TGG GGT CAG CGG TGC AGC AGG GGG GGT-3¢ (XbaI sites underlined; His-tag in italic) ... activity by bis-ANS was decreased upon phosphorylation. A possible explana- tion for this apparent discrepancy could be that PKA phosphorylation induces a conformational change that inc...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt
... McIntosh EM & Haynes RH (1984) Isolation of a Saccharomyces cerevisiae mutant strain deficient in deoxycytidylate deaminase activity and partial charac- terization of the enzyme. J Bacteriol 158, ... Coordinated closure of the active site and rearrangement of the main chain and side chains in the active site appear as key players in a slow transformation from an ina...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
... 3¢-GG AACCACGAAAGTAG CAATC AGA ACACTA AAACC A GGTACAATGATGC-5 ¢ . All vector inserts were sequenced prior to use. CDK4 pY17 antibody generation Murex lop-eared rabbits were injected with a phosphopep- tide ... C-YES and C-SRC were assayed for Y17 kinase activity using the plate format assay at various concentrations of the Src family kinase inhibitor PP2. Values are the averag...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Reconstitution of coupled fumarate respiration in liposomes by incorporating the electron transport enzymes isolated from Wolinella succinogenes docx
... treated in the same way as described above. The reaction was started by the addition of fumarate instead of DMN, and the consumption of fumarate was recorded in a se parate experiment in the absenc ... technical reasons, the H + /e ratio of apparent proton translocation can only be measured at vanishing Dp.Itis generally thought that the same ratio is valid in the...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Complementation of coenzyme Q-deficient yeast by coenzyme Q analogues requires the isoprenoid side chain ppt
... this was a valid assumption as loss of area from the CoQ 2 H 2 and decylQH 2 peaks was compara- ble with the gain in size of the CoQ 2 and decylQ peaks. Determination of the fraction of CoQ 2 and ... library A genetically stable double-knockout Dcoq2DORF MATa library was created using the approach of Tong and coworkers [14]. We utilized the commercially available dip...
Ngày tải lên: 06/03/2014, 11:20
Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
... upregulation of both RGS mRNA was found only after activation of the TLR3 signalling pathway. This upregulation of RGS1 and RGS2 mRNA is due to the TRIF pathway. We veri- fied the data by stimulation ... stimu- lates the GTPase activity of several members of the Ga i subfamily but is ineffective against Ga s [21], whereas RGS2 does not interact with Ga i ,Ga o ,Ga s a...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf
... counteracting the glycosylation pattern associated to malignancy. We found in fact that NeuAca2-3Galb1-3[Fuca1-4]Glc- NAcb1-3Gal and NeuAca2-3Galb1-3GlcNAcb1-3Gal are the main oligosaccharides released by ... b1,4- and b1,3galactosidases, giving rise to a disaccharide and a monosaccharide, and was thus identified as a mixture of Galb1-4[Fuca1-3]Glc- NAcb1-3Gal and Galb1-3[Fuca1-4...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Inhibition of pea ferredoxin–NADP(H) reductase by Zn-ferrocyanide docx
... 5 ¢-GCAACACCATGGCTTCTTACAAAG TGAAA-3¢ and Fdlw 5¢-CCACAAGCTTGATATCATA TCATAGCATAGCAGT-3¢ and the full length p ea Fd precursor cDNA as template. To facilitate the cloning process, NcoIandHindIII ... Fd-NADP + reductase was purified according to published procedures [32]. Fld from Anabaena was k indly provided by M. Medina (University of Zaragoza, Zaragoza, Spain). ApoFld from Anabaena...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc
... [40]. Two major pathways for caspase activation have been identified: the receptor pathway and the mito- chondrial pathway; both pathways trigger a cascade of downstream caspases that induces DNA fragmentation [2]. ... mitochondrial pathway [47]. Caspase-9 is also cleaved by caspase-3 at another cleavage site. How- ever, this fragmentation does not have caspase activity. It enha...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx
... indicated strains were grown to stationary phase and then lipids were extracted and separated by TLC. The amount of SE of the wildtype strain was set at 100%. Data are mean values of three independent ... substratum [10]. Among the Cdc42p effectors that regulate cell polari- zation are Ste20p and Cla4p, both members of the p21- activated kinase (PAK) family [4,11]. Cla4p p...
Ngày tải lên: 18/02/2014, 13:20