Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx

... 5909 Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera 1 , Theo Wallimann 2 , Subhankar Ray 1 and Manju Ray 1 1 Department of Biological ... homo- cysteine to methionine. Tissue metabolism of arginine and methionine is of high importance for the effective regulation of cell death...

Ngày tải lên: 07/03/2014, 04:20

... observed by Hoechst 33342 staining in these cells (Fig. 3A), and the rate of apoptotic cells was approximately threefold higher in the Hsp105a over-expressing cells than in F9 or V1 cells (Fig. 3B). However, ... treatment, cells were incubated in medium containing 50 lgÆmL )1 neutral red at 37 °C for 3 h, then fixed with 1% formaldehyde containing 1% CaCl 2 for 1 min. The...

Ngày tải lên: 21/02/2014, 03:20

... in intracellular Ca 2+ levels are involved in the action of neokyotorphin in L929 fibroblasts. Protein kinases involved in the action of neokyotorphin To study the involvement of non-MAPK protein ... (MAPK ⁄ ERK) in the effect of neokyotorphin. In general, the action of neokyotorphin in L929 cells appears be similar to that of cAMP. Results Effect of neokyotorphin...

Ngày tải lên: 07/03/2014, 11:20

... randomness in the binding of substrate and coen- zyme in each direction of the reaction catalyzed by AfM2DH. By way of comparison, an earlier study on M1PDH from A. niger also reported random binding of ... adopts an expanded Rossmann fold, and provides the residues for binding the coenzyme. The C-terminal domain has a lar- gely a-helical structure, and serves mainly in su...

Ngày tải lên: 14/03/2014, 23:20

... in the absence o f inositol and repressed in t he presence of inositol [1]. UAS INO -containing genes are activated by two tran- scription factors – Ino2p and Ino4p [2] – and repressed by a negative ... able to restore wild-type regulation of t he INO1 gene, derepression of the gene in medium lacking inositol, and repression in medium containing inositol. N one of the...

Ngày tải lên: 30/03/2014, 15:20

... linear gradient of 50–100% sol- vent B in 2 min, maintenance at 100% solvent B for 6 min, and linear gradient of 100–5% solvent B in 2 min. The col- umn was maintained at 30 °C and the flow rate ... H 2 O containing 0.05% TFA). The peptides were separated using a solvent system of solvent A and solvent B (80% ACN ⁄ 20% H 2 O containing 0.04% TFA): linear gradient of 5–50% sol...

Ngày tải lên: 16/02/2014, 14:20

... detectable binding to CRP at the concentration and during the period of observation used in the experiment. Interaction analyses using increasing concentrations of the native and of the low-pH ... Increased binding of low-pH buffer-exposed IVIg to human factor H. In both panels, binding of the native IVIg is indicated by a solid line, and that of the low-pH buffer-expose...

Ngày tải lên: 16/02/2014, 15:20

... 3729–3734. Supporting information The following supplementary material is available: Fig. S1. Amino acid alignment of linker region and ligand-binding domain (LBD) of ecdysone receptors. Fig. S2. Amino acid ... nonsteroidal agonists bind exclusively to the EcR ligand-binding domain (LBD), although the presence of USP increases ligand-binding affinity [12–15]. The diversity of ligand-...

Ngày tải lên: 18/02/2014, 08:20

... to activation of a series of signalling proteins, and to the expression of pro- and in ammatory cytokines. There is evidence that the heteromeric guanine nucleotide- binding regulatory protein (G protein) ... [1,2]. TLR contain two major domains: the extracellular ligand-binding domain, characterized by leucine-rich repeats and the intracellular Toll ⁄ IL-1 receptor domain (TIR d...

Ngày tải lên: 18/02/2014, 13:20

... the GS1 ⁄ ferredoxin-GOGAT cycle is the main pathway of inorganic nitrogen assimilation and recycling into glutamine and glutamate, and preconditions amino acid inter- conversion and remobilization. Abbreviations AS, ... the main nitrogen compounds in the phloem exudates, amounting to 51% of the total amino acids in the light and 56% in the dark (Fig. 7A). The total amount...

Ngày tải lên: 18/02/2014, 18:20